FastQCFastQC Report
Sat 13 May 2023
EGAF00001721735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83411415
Sequences flagged as poor quality0
Sequence length30-51
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCA2851070.34180813261590154No Hit
ACCTATTTATCTATTTATTAACTTTAAATCCAAATTATAAAACCAATTA2181500.26153494698537366No Hit
AAATAATCCAAATCAATTTCTATCTACTTCAAATTCCTCCCTATACAAA1779340.2133209225619779No Hit
AACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT1685290.20204548741919798No Hit
AATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTA1546150.18536431734193695No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACCA1534550.18397362039716025No Hit
AACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG1137390.1363590343120303No Hit
AACTCTACCATCTTAACAAACCCTATTCTTAAATAAATATAAATATAAT1123410.1346830047182391No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCTTATTAACCA977600.1172021838977315No Hit
ACTAATTTTTTATATTTTTAATAAAAACAAAATTTCACCATATTAACCA945730.11338136392962522No Hit
AATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCG918390.11010363509598776No Hit
ACTACATTTATTTATTTATTTTTTTAATTATTTTACTTTTTTTTAATTT858210.10288879525662045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)