FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001721751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82989998
Sequences flagged as poor quality0
Sequence length30-51
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACCA3555260.4283962026363707No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCTTATTAACCA2381630.28697795606646476No Hit
AATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCG1501190.18088806316153905No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCATATTAACCA1487640.1792553362876331No Hit
AACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG1463030.1762899186959855No Hit
ACTAATTTTTTATATTTTTAATAAAAACAAAATTTCACCATATTAACCA1172130.14123750189751783No Hit
AAATAATCCAAATCAATTTCTATCTACTTCAAATTCCTCCCTATACAAA1099820.1325244037239283No Hit
AACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT1053520.12694541816954857No Hit
ACTAATTTTTTATATTTTTAATAAAAACAAAATTTCACCGTATTAACCA1027360.12379323108309992No Hit
ACTAATTTTTATATTTTTAATAAAAACAAAATTTCACCATATTAACCAA1001320.12065550357044232No Hit
AATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCA939220.1131726741335745No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACTATATTAACCA900100.10845885307769257No Hit
ACTAATTTTTATATTTTTAATAAAAATAAAATTTCACCATATTAACCAA891940.10747560205990124No Hit
AATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA873970.10531028088469167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)