FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001721812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22520325
Sequences flagged as poor quality0
Sequence length30-51
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG865850.3844749132172826No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGTG501990.22290530887098653No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGTG486170.21588054346462585No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAAT436190.19368725806576947No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTTA417550.18541029048204233No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAAT383670.1703661026206327No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAAT357750.15885649962866877No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAAT304580.13524671602208227No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAAT296670.13173433331890191No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGG282780.12556657153038422No Hit
TGGAATTGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAAT263910.11718747398183642No Hit
TGGTTAATTTTTTGTATTTTTAGTAGAGATGGGGTTTTATTGTGTTAGTTA256500.11389711294130969No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTTA246040.10925241975859586No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATT242010.10746292515760762No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAAT237040.10525602983083059No Hit
TGGGAGGTGGAGTTTGTAGTGAGTTGAGATTATGTTGTTGTATTTTAGTTT228820.101605993696805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)