FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001721845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83828163
Sequences flagged as poor quality0
Sequence length30-51
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACCA4469140.5331310910391773No Hit
AACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG3887840.4637868540671707No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCTTATTAACCA2889770.34472543553173174No Hit
AATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCG1818500.21693186811215223No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCATATTAACCA1725510.20583893744635676No Hit
ACTAATTTTTATATTTTTAATAAAAACGAAATTTCACCATATTAACCAA1204080.14363669164502627No Hit
ACTAATTTTTATATTTTTAATAAAAACAAAATTTCACCATATTAACCAA1202930.1434995062458902No Hit
ACTAATTTTTATATTTTTAATAAAAATAAAATTTCACCATATTAACCAA1159580.13832821315671678No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACTATATTAACCA1159460.13831389815854608No Hit
ACTAATTTTTTATATTTTTAATAAAAACAAAATTTCACCGTATTAACCA1063240.12683565545865536No Hit
ACTAATTTTTTATATTTTTAATAAAAACAAAATTTCACCATATTAACCA958770.11437325663452746No Hit
ACTAATTTTTTATATTTTTAATAAAAATAAAATTTCACCGTATTAACCA893880.10663242137370944No Hit
ACTAATTTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACC890740.10625784558824224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)