FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001721851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82431149
Sequences flagged as poor quality0
Sequence length30-51
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAATCCAAATCAATTTCTATCTACTTCAAATTCCTCCCTATACAAA2813960.3413709543221337No Hit
AACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT2680540.32518532527066923No Hit
AACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG2603480.31583691742547465No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACCA2423700.2940272008097327No Hit
AACTCTACCATCTTAACAAACCCTATTCTTAAATAAATATAAATATAAT2037310.24715293001678287No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCTTATTAACCA1614680.19588226290525199No Hit
ATAAAAATAACAATAATTATAATAACAAAAATAAAATATACTCATATAT1484010.18003024560533543No Hit
AATATCAAAAAAATACCTTAAATAACCTCTAAAACTCAAAAATAAAAAA1454630.17646605896516132No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCATATTAACCA1113880.13512853011426543No Hit
ACTAATTTTTATATTTTTAATAAAAATAAAATTTCACCATATTAACCAA933160.11320477893617617No Hit
ACTAATTTTTATATTTTTAATAAAAACAAAATTTCACCATATTAACCAA907740.11012099321823113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)