FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28071829
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTA7481172.665009821768293No Hit
CGTACT4572841.6289782899432736No Hit
ATCCAC4481121.5963049646676033No Hit
TATTCT4355811.5516659067708056No Hit
GAGATA4143021.4758639346228561No Hit
GTAAGA3858381.374466907731591No Hit
AGTGCA3526241.2561490026175353No Hit
ATTGCT3479811.2396092894410264No Hit
TTCACA3471771.2367452081586845No Hit
TCATGT3441671.2260227148006637No Hit
ACCGCT3368011.1997828855398058No Hit
AGAACG3361131.1973320299151153No Hit
CGAATT3336211.1884548028559165No Hit
CTGGAG3169431.1290429276980847No Hit
CTACCG3144931.120315316825277No Hit
GGATTC3144451.1201443268979732No Hit
AATTGC3042381.0837840313148102No Hit
TTCTAC2997591.067828533723257No Hit
TCTTCA2793570.9951506900387574No Hit
CTTGTA2679460.9545013971123861No Hit
CCAGCG2673430.95235333615063No Hit
GATTCA2648520.9434796713815833No Hit
ATGTGA2589420.9224265365822796No Hit
GCGCAG2482010.8841639780578601No Hit
TGTGAT2459800.8762521316298985No Hit
CTGAGG2317430.825535806733505No Hit
CACATT2316330.8251439548167667No Hit
GTGGTG2303590.8206055971629066No Hit
TAGAGC2300770.8196010313399957No Hit
TAGCTT2275900.8107416157315578No Hit
TTCCAT2263490.8063208136527192No Hit
GAACCG2156580.7682363696359079No Hit
CCTGAC2027580.7222828266729611No Hit
AATCGG2020150.7196360450899013No Hit
CAAGCT2008470.7154752901921709No Hit
AGGACT1981310.7058001101388869No Hit
CGCTTG1969640.7016429175313087No Hit
ATTCGA1950630.6948710039520403No Hit
GTACTT1891600.6738428051838019No Hit
AGCCTA1830990.6522517645715211No Hit
GGCTAC1826070.6504991178166553No Hit
AATGTA1765310.628854642852092No Hit
AACATA1750370.6235325813647554No Hit
AGTTAG1735970.6184028835456357No Hit
CGAAGC1719330.6124752327324308No Hit
GCTCAA1716060.6113103638526723No Hit
CCGGAA1708490.6086137102074823No Hit
AACAAT1687600.6011720860796067No Hit
CAGTAG1687200.60102959447352No Hit
CAAGGA1678310.597862718528244No Hit
TGCGAC1647930.5870404810459625No Hit
ACCAGG1644870.5859504202593995No Hit
GCGTAT1642770.5852023393274447No Hit
AGTCAA1637820.5834390057021221No Hit
TACGAA1600080.5699949226678461No Hit
CCACTT1593510.5676544980378727No Hit
CATTAA1547380.5512216535659291No Hit
CCGTCG1545480.5505448184370174No Hit
GCGGCT1535630.5470359626371335No Hit
CCATGA1529000.5446741642662472No Hit
ACTAGC1517820.5406915238761251No Hit
GCCTGG1512780.5388961296394332No Hit
TACAGT1489760.530695737709146No Hit
TAAGTC1478200.5265777302932416No Hit
CCTTAT1467060.5226093390637282No Hit
GGCAGG1464810.5218078237794908No Hit
CAGTCC1457200.5190969209736922No Hit
CTATGC1452960.5175865099491737No Hit
TCGGAC1442930.5140135329265507No Hit
ATCCGG1429050.5090690741953436No Hit
AAGTTG1414330.5038253830913547No Hit
CCGACT1390070.4951832671821989No Hit
GAATAC1386130.4937797248622453No Hit
GGCGTT1380180.49166016222170633No Hit
GACCGA1376280.49027086906236145No Hit
GGTGGT1351570.4814684500963582No Hit
TCCTCC1332540.4746894119367855No Hit
GACTAA1297550.4622249586943551No Hit
TTAGTA1293760.46087485072668405No Hit
GACTTG1288500.4590010861066445No Hit
GCCATA1287520.4586519816717322No Hit
TGACCA1287340.4585878604489932No Hit
AGTTCC1282700.45693495781838794No Hit
AAGCAT1279810.45590545596441184No Hit
CTGTTC1276170.45460878234902324No Hit
TAATGA1248930.44490510397452193No Hit
AACTCT1206180.4296763135740104No Hit
TTAGCG1204220.42897810470418585No Hit
GAGCAC1190680.4241547638381525No Hit
ATGGCC1182780.42134055461794095No Hit
ACCTGC1182380.4211980630118543No Hit
AGATGC1173530.41804543622718704No Hit
GCCAAT1168170.4161360487056258No Hit
TGATAA1166720.41561951663356167No Hit
TCAATG1143820.40746187218510055No Hit
TGCGGA1123600.4002589214974201No Hit
TCAAGC1115390.39733428128249143No Hit
CGACAA1111150.3958238702579729No Hit
GATCAG1094440.38987128341370275No Hit
GGCCAG1092430.3891552630931173No Hit
GAGCCA1068600.38066632566050473No Hit
AACCTT1067770.3803706555778749No Hit
GCTTCT1066080.37976862854215876No Hit
TTCGTT1043170.37160742180354545No Hit
ATGCCG1035020.3687041553295298No Hit
ACGTAA1031960.3676140945429669No Hit
TGACAC1021370.3638416292718227No Hit
GCACAT1018860.3629474944436289No Hit
CGTTGC1015650.36180399930478346No Hit
CTTCAC1015000.36157245044489267No Hit
CGAGCC1008260.3591714668823325No Hit
CCTGCT1005640.35823814686246486No Hit
ACTAAT1000050.3562468266674038No Hit
GTCTCA984770.35080364731489355No Hit
ATACCT983700.35042248226861167No Hit
GGTACC955240.340284204495546No Hit
GTTGGC954060.3398638542575904No Hit
CAGATC951390.338912722786962No Hit
TGTGTG947530.33753767878822577No Hit
CATAAC945780.3369142780115966No Hit
AGACGG935520.33325936831547387No Hit
CATACG933890.3326787150206707No Hit
CTGTAT914470.3257607475451635No Hit
TGCATA909300.3239190435364935No Hit
AGGTCA906730.3230035349673867No Hit
TGGCAT904800.3223160129680186No Hit
AAGAGG896560.3193806858826334No Hit
TCCACG883300.31465708914086077No Hit
CACCTA869360.30969125666874076No Hit
TCGTGG853130.30390965975177464No Hit
AGGCTT848160.30213920154614793No Hit
ACTTGA835310.2975616587006141No Hit
ACGAAC824120.2935754560203398No Hit
CTATTA816000.2906828764167807No Hit
GAGTCG790780.28169878065301696No Hit
ATTCAT785900.27996038305875975No Hit
AACTAG779540.2776947665219819No Hit
CTTAGC777520.27697518391124426No Hit
CATGAG772280.27510854387150907No Hit
ACAGTG756130.2693554452757603No Hit
ATTGAA750110.26721094660415606No Hit
TACGGC734470.26163952480616776No Hit
TATTGG732070.2607845751696478No Hit
ACACGA731230.26048534279686586No Hit
GATCCT728260.2594273426216724No Hit
GAAGAT705110.25118064091940717No Hit
TACTCG689130.24548810125624518No Hit
GACACG677090.24119910391303678No Hit
GCTGTC655940.2336648602412048No Hit
GACCAT654880.23328725748507517No Hit
GTCTGC645730.23002776199584288No Hit
CTCCGC645220.2298460851980824No Hit
CAGCCT642620.22891988975851912No Hit
TTGAAT635390.22634435397850278No Hit
ACAGCC630690.2246700776069846No Hit
TATGGT626240.2230848584892705No Hit
GTTACA605680.21576078993641631No Hit
GAGAAG592570.21109062754692612No Hit
CCTCGG590720.21043160386877532No Hit
TTCTGT587970.20945197407692956No Hit
GAGTGA582720.20758177174704223No Hit
CTAGTT573410.20426527961537524No Hit
TCCAGA568010.2023416429332054No Hit
GGATCG525080.1870487313099549No Hit
CGAGAG517520.18435563995491708No Hit
GGCCGT500390.178253436924256No Hit
GTACGC497570.17724887110134507No Hit
AGTAGG493070.17564584053287016No Hit
AGGTGT490810.17484076295848056No Hit
TCTCGC490680.17479445318650239No Hit
CCGCTC487040.17349777957111379No Hit
AAGGCG479170.17069425722135884No Hit
ATCAGC466130.1660490308629338No Hit
AAGCGC448940.1599254540913597No Hit
TATAAG444000.1581656827561895No Hit
TTAGGC422000.15032864442142335No Hit
CAGGAC418970.14924927050531692No Hit
GGAGGC392950.13998019152937988No Hit
GCATAA390220.13900768631783844No Hit
CGTCAG389140.13862295898140445No Hit
GGTAAT355660.12669641155195124No Hit
ACGCTA334200.11905173688540208No Hit
AGAGGT317410.11307065171991465No Hit
CAACGG317250.11301365507747999No Hit
CTCGTC310490.1106055469346155No Hit
CGTGAA299020.10651960013008058No Hit
GTCGAG281930.10043164626002815No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)