FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26252651
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC3913721.4907903967488845No Hit
TACGAA3601681.3719300195625959No Hit
CAGTCC3439841.310282912000011No Hit
AGCCTA3253341.239242467360725No Hit
CCTTAT3100361.1809702570608964No Hit
ACTAGC3052411.1627054349673105No Hit
GAATAC2839401.0815669625136144No Hit
ATGCCG2730631.0401349562754634No Hit
CCGTCG2618940.9975906814134695No Hit
CAGTAG2608160.9934844294391451No Hit
CAAGGA2596700.9891191560044736No Hit
GCTTCT2532990.9648511306534338No Hit
GGCGTT2420770.9221049714179341No Hit
GATCCT2400610.9144257469464703No Hit
CGTTGC2348260.8944849036388745No Hit
GCCAAT2290840.8726128267960443No Hit
GAGCAC2282410.8694017225155661No Hit
TAGCTT2263810.8623167237472513No Hit
ACTTGA2199350.8377630129620053No Hit
TGACCA2179100.8300495062384367No Hit
CATTAA2178850.8299542777603679No Hit
CTTCAC2165490.824865267892374No Hit
AGTGCA2147730.8181002368103701No Hit
GATCAG2052880.7819705522310871No Hit
GGCCAG2024210.771049750366163No Hit
GAACCG2014740.7674424956169188No Hit
CGACAA1990940.758376744504774No Hit
GACCGA1966460.7490519719322822No Hit
GGTGGT1945140.7409308873225794No Hit
GTACTT1929020.7347905550567064No Hit
GAAGAT1924150.7329355043039273No Hit
CGAAGC1911850.7282502631829448No Hit
TCTCGC1902310.7246163444598415No Hit
TACTCG1876630.7148344751926196No Hit
AGGACT1849770.7046031275089133No Hit
CGAGAG1844570.7026223751650833No Hit
TTCCAT1844420.7025652380782421No Hit
ACACGA1844310.7025233375478919No Hit
AATTGC1827220.6960135187871123No Hit
AAGTTG1804280.6872753536395239No Hit
AACAAT1803100.6868258752230394No Hit
TCAATG1801560.6862392677981358No Hit
TACAGT1778900.6776077585459845No Hit
TCGGAC1775340.6762517050182856No Hit
CGAGCC1760700.6706751253425797No Hit
AGTTAG1728330.6583449420022381No Hit
CTGAGG1716800.6539530045937075No Hit
AATGTA1702070.648342142665897No Hit
AGTTCC1690570.6439616326747345No Hit
TTAGCG1679590.6397791979179551No Hit
TGCGGA1660670.6325723066977121No Hit
CTATTA1658380.6317000138386024No Hit
CCTGCT1656260.6308924763445795No Hit
TACGGC1632390.6218000612585753No Hit
GACTTG1612670.6142884389085125No Hit
GGACTA1594850.6075005529917721No Hit
TGTGAT1582980.602979104853068No Hit
GGTAAT1576880.6006555299881905No Hit
CTCCGC1555540.5925268271002422No Hit
GGCTAC1546830.5892090669243271No Hit
AGACGG1545730.5887900616208245No Hit
GACCAT1531380.5833239469796784No Hit
GCGCAG1526880.5816098343744409No Hit
GAGCCA1523760.5804213829681429No Hit
GGCCGT1522990.5801280792556911No Hit
GCCATA1491950.5683045114186754No Hit
ATTGAA1461500.5567056827899018No Hit
GTGGTG1454530.5540507128213452No Hit
CCGACT1427380.543708900103079No Hit
AAGCGC1419130.5405663603268105No Hit
GTCTGC1388080.5287389833506719No Hit
GGATCG1381410.5261982875557977No Hit
TCCACG1379650.5255278790701937No Hit
TCCTCC1368640.5213340168960461No Hit
GATTCA1357280.5170068348526021No Hit
CGTCTC1330600.5068440516731053No Hit
TTCGTT1325100.5047490251555928No Hit
TGTGTG1318300.502158810552123No Hit
ACAGTG1317480.5018464611440574No Hit
TCATGT1315910.5012484263017857No Hit
CCAGCG1315160.5009627408675794No Hit
ATGGCC1314840.5008408484156515No Hit
ATTCGA1314640.5007646656331964No Hit
TGATAA1314240.5006123000682865No Hit
AACATA1309980.49898960680199494No Hit
CAGGAC1308370.49837633540323223No Hit
TAGAGC1308120.4982811069251635No Hit
CTAGTT1285160.48953532349932966No Hit
GTAAGA1263360.48123140021173477No Hit
TCCAGA1251610.47675566174250367No Hit
CCATGA1250230.4762300005435641No Hit
CACATT1245780.47453493363394045No Hit
ATTGCT1241670.47296937745449025No Hit
TATTGG1233740.46994873013014954No Hit
ACAGCC1232310.46940402323559627No Hit
TTCTAC1232230.46937355012261434No Hit
CAGATC1213920.46239901638885916No Hit
TCGTGG1202920.45820896335383426No Hit
CCACTT1200420.4572566785731468No Hit
ATCAGC1197320.4560758454450943No Hit
TATAAG1185320.45150487849779436No Hit
CGTACT1179560.4493108143630904No Hit
CTTGTA1167200.44460271840737153No Hit
CGAATT1138490.4336666799859564No Hit
CGCTTG1133190.43164783625089903No Hit
GTCTCA1128700.4299375327847843No Hit
AGAGGT1126710.429179514099357No Hit
ACTAAT1118930.42621600386185765No Hit
CCTGAC1110200.4228906254076969No Hit
ACCGCT1106820.4216031363842075No Hit
ATTCAT1089940.41517330954500553No Hit
CTTAGC1083130.4125792858024129No Hit
AACTCT1024340.390185356899766No Hit
ACGTAA1017610.38762180627015536No Hit
TCTTCA1016810.38731707514033537No Hit
GAGATA1014010.3862505161859654No Hit
TGCGAC991520.37768376229890077No Hit
TTAGTA985050.3752192492864816No Hit
GCCTGG980080.3733261071424749No Hit
GTTGGC979580.3731356501863374No Hit
TTAGGC968670.3689798794034172No Hit
CTGTTC963950.36718196573747924No Hit
TTCACA955670.36402799854384227No Hit
AGAACG955030.3637842136399863No Hit
CAACGG935860.3564820939416747No Hit
CTACCG935490.35634115579413295No Hit
CCTCGG931080.3546613254410002No Hit
GCACAT930280.3543565943111803No Hit
AATCGG917680.34955707901651534No Hit
AGGTGT915100.3485743211228458No Hit
GCGGCT913930.3481286518454841No Hit
AGGTCA896670.3415540777196177No Hit
AGTCAA888910.3385981857603638No Hit
ACCTGC884540.3369335919637221No Hit
ATGTGA877300.3341757752388511No Hit
ATCCAC869350.33114750963626494No Hit
CATGAG853380.32506431445723327No Hit
AAGCAT852450.32471006451881756No Hit
CACCTA849250.3234911399995376No Hit
AGTAGG847890.3229730970788436No Hit
GCATAA844970.3218608284550006No Hit
ATACCT834050.31770124853295767No Hit
AACCTT831590.3167642003087612No Hit
TGCATA821610.31296267946425677No Hit
GCTCAA821060.31275317681250553No Hit
TTACGT820230.3124370182653173No Hit
CTGGAG805720.30690995739820714No Hit
GTCGAG800340.30486064055016765No Hit
GACTAA790280.30102864659268125No Hit
ACGAAC784370.29877744537113604No Hit
ACCAGG783930.29860984324973505No Hit
CGTGAA782990.29825178417219655No Hit
TAAGTC781080.29752423859975136No Hit
CCGGAA780710.29738330045220956No Hit
GACACG765620.29163530951597993No Hit
AGATGC762850.2905801779789782No Hit
TATGGT762520.2904544763879275No Hit
CTGTAT756390.2881194741056818No Hit
CATAAC755020.28759762204586503No Hit
TTCTGT732600.27905753213265966No Hit
CAGCCT725520.2763606616337527No Hit
GGCAGG694760.26464374969217397No Hit
AAGAGG690770.26312390318219675No Hit
CATACG689000.26244968555746995No Hit
TTGAAT672800.2562788801786151No Hit
AAGGCG653720.24901104273240826No Hit
TCTCTG633810.24142704673901313No Hit
CTATGC629170.23965960618605717No Hit
TATTCT613910.23384685988474077No Hit
GAGAAG612490.23330596212931032No Hit
AACTAG589470.22453732386873995No Hit
TGGCAT589350.22449161419926697No Hit
ACGCTA569440.21690761820587187No Hit
GCGTAT568150.2164162392590371No Hit
AGGCTT562390.21422217512433317No Hit
GAGTCG542420.20661532429620152No Hit
GCTGTC530170.20194912887083288No Hit
TAATGA529420.20166344343662662No Hit
ATCCGG518730.19759147371440697No Hit
TGACAC503810.19190823814326408No Hit
CGTCAG501670.1910930823709956No Hit
GGAGGC484910.18470896520126673No Hit
TCAAGC429410.16356824307000462No Hit
CAAGCT409390.15594234654625927No Hit
GAGTGA393800.15000389865389213No Hit
CTCGTC387340.1475431947805957No Hit
GTACGC379480.14454921143011423No Hit
CCGCTC362040.13790607280003836No Hit
GGATTC337010.12837179757579528No Hit
GTTACA320750.12217813736220393No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)