FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25318268
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGCA5637062.2264793152517384No Hit
GGTAAT3837391.5156605499238731No Hit
TACAGT3439781.3586158421263257No Hit
GGTGGT3339021.318818491059499No Hit
CTTCAC3309901.307316914411365No Hit
CAGTCC3308361.3067086579540117No Hit
GATTCA3138741.239713553865533No Hit
CCGTCG3061481.2091980383492267No Hit
CAGTAG2971331.1735913372905287No Hit
AGTTAG2924251.1549960684514438No Hit
ACACGA2867451.1325616744399736No Hit
TCGGAC2856831.1283670747145895No Hit
CTCCGC2842061.122533342328156No Hit
ATTCGA2789091.1016116900255577No Hit
TTCCAT2746661.0848530397102993No Hit
GAATAC2710451.070551113527987No Hit
CCGACT2696641.0650965539980854No Hit
TGATAA2635571.0409756307184994No Hit
TAGCTT2633341.0400948437705138No Hit
AATTGC2561051.0115423377302113No Hit
GTACTT2480130.9795812256983772No Hit
TTCTAC2428020.9589992490797554No Hit
GTCGAG2334130.9219153537674851No Hit
CGAGCC2317280.9152600801918993No Hit
ATGCCG2286390.9030594035895347No Hit
GGCGTT2242730.8858149380518445No Hit
TCATGT2237290.8836662918648306No Hit
ACTTGA2227960.8799812056654113No Hit
AGGTGT2219330.8765725996738798No Hit
AGTTCC2193620.8664178766098851No Hit
CAGATC2162020.853936770082377No Hit
CTGAGG2133830.8428025171390081No Hit
GCGTAT2121360.837877219721349No Hit
GGATCG2106680.8320790347902155No Hit
GATCAG2046640.8083649323879502No Hit
CCTGAC2031980.8025746468913276No Hit
ATTCAT2010200.7939721627087603No Hit
AACATA1999910.7899079036528092No Hit
GGCAGG1943990.7678210847598264No Hit
CGACAA1899690.7503238373177817No Hit
GACTTG1895170.7485385651182774No Hit
CTTAGC1883300.7438502507359508No Hit
CGTACT1877660.7416226102038259No Hit
GCCATA1857000.7334624943538792No Hit
GGCTAC1843550.7281501246451771No Hit
TACTCG1825580.7210524827369708No Hit
GGCCGT1819670.7187181998389464No Hit
TCAATG1788040.7062252441596716No Hit
GAAGAT1783930.7046019103676444No Hit
ATGGCC1780200.7031286658313278No Hit
AAGCGC1752140.6920457592122811No Hit
GGCCAG1711920.676159996410497No Hit
TCTCGC1693760.6689873098744353No Hit
TGCGAC1613710.6373698232438333No Hit
TTCACA1581570.624675431984526No Hit
CGTCTC1550620.612451057078628No Hit
TGCGGA1506520.5950328039816942No Hit
TTAGGC1503490.5938360396532654No Hit
ATTGCT1500490.5926511244766032No Hit
ACAGTG1499520.5922680019028158No Hit
AGACGG1494570.5903128918613232No Hit
AGAACG1477730.583661568002993No Hit
TGACAC1459620.5765086300532091No Hit
TTCGTT1457630.5757226363193565No Hit
GACCAT1434380.5665395437002247No Hit
CAAGGA1410190.5569851776590721No Hit
CGTTGC1394840.5509223616718174No Hit
GTGGTG1336100.5277217225127722No Hit
AGGACT1301330.5139885556152577No Hit
ATGTGA1291320.5100348886424616No Hit
TCCAGA1283930.5071160475906172No Hit
CTAGTT1262210.4985372617115831No Hit
ATACCT1261250.4981580888550512No Hit
CGAAGC1242800.4908708605185789No Hit
CCAGCG1237010.48858397422762095No Hit
AGGTCA1217030.480692439151051No Hit
GTTGGC1208230.4772166879661753No Hit
GCTTCT1201920.47472441637792917No Hit
AACTAG1196400.47254417245287084No Hit
TAGAGC1180850.46640236212050523No Hit
TGACCA1164970.4601302111187069No Hit
ACCAGG1148400.4535855296262762No Hit
AAGCAT1144590.45208068735191526No Hit
ACCGCT1139750.4501690242002336No Hit
GACCGA1137580.44931193555578125No Hit
AACTCT1117630.4414322496309779No Hit
AGCCTA1115810.4407134010904696No Hit
GAACCG1105420.4366096448619629No Hit
AATCGG1102130.43531018788489007No Hit
GACACG1098210.4337618987207182No Hit
TGTGTG1078900.42613499470026933No Hit
CCTGCT1070850.42295547230955927No Hit
GTCTGC1061160.41912819628894044No Hit
CCACTT1037180.4096567743101543No Hit
GAGCAC1024230.40454189046422917No Hit
AGTCAA1010460.3991031298033499No Hit
CCTTAT1005600.3971835672171572No Hit
TCTTCA1002540.3959749537369618No Hit
AATGTA1002060.39578536730869585No Hit
GAGATA988040.390247863716428No Hit
GCTCAA985320.389173540622921No Hit
GATCCT980350.38721053114691734No Hit
AGAGGT964700.3810292236419964No Hit
CCGGAA964460.3809344304278634No Hit
GAGCCA962480.38015238641126636No Hit
TATTGG943770.37276246542615No Hit
CAGGAC921440.3639427467945279No Hit
CGAATT906400.358002372042195No Hit
TCCACG905320.3575758025785966No Hit
CTTGTA904580.35728352350168663No Hit
CTATTA855490.33789436149423807No Hit
GCCAAT847760.3348412300557052No Hit
GGTACC839060.3314049760433849No Hit
GCCTGG838020.33099420544880875No Hit
CTGTAT837360.33073352410994306No Hit
ACGTAA832140.3286717717025509No Hit
GTAAGA815560.32212314049286467No Hit
GCGGCT804580.31778635094628116No Hit
CATTAA798300.3153059285098017No Hit
TCCTCC781820.3087967944726709No Hit
AAGTTG780890.3084294707679056No Hit
AACAAT762820.30129233168714387No Hit
CATAAC761480.3007630695749014No Hit
ATTGAA715270.28251142613704855No Hit
ACAGCC714340.2821441024322833No Hit
TATAAG712300.281338360112153No Hit
CATGAG702700.2775466315468341No Hit
GGATTC701170.2769423248067364No Hit
GTCTCA699320.2762116271144614No Hit
CGTGAA689590.2723685522248204No Hit
TTACGT684560.2703818444452835No Hit
ACTAAT681290.2690902869027218No Hit
ACTAGC678490.26798436607117043No Hit
GCGCAG669820.26455996121061676No Hit
TACGAA667860.26378581662853084No Hit
ACGAAC651920.25748996732319923No Hit
CGAGAG645110.2548002098721761No Hit
CTGGAG643790.2542788471944447No Hit
CAACGG628960.2484214165044781No Hit
ATCCAC621430.24544727941105607No Hit
TGGCAT615530.24311694623028718No Hit
ATCAGC573030.22633064789423984No Hit
TATGGT570200.22521287791092184No Hit
GCACAT554940.219185609378967No Hit
TTCTGT532910.21048438226501115No Hit
TACGGC528090.20858061854784066No Hit
CTACCG523060.20659391076830375No Hit
TTAGCG520000.20538529728810836No Hit
AGATGC519330.2051206662319871No Hit
ACCTGC513890.20297202004497306No Hit
CACCTA506230.199946536627229No Hit
GAGTCG505860.199800397088774No Hit
GACTAA495510.19571243972928953No Hit
AGTAGG489070.19316882181672143No Hit
CGCTTG487960.19273040320135643No Hit
TATTCT481310.19010384122642196No Hit
GGACTA469180.18531283419545128No Hit
GCTGTC468650.18510349918090763No Hit
CCATGA464240.18336167387121427No Hit
CGTCAG450400.17789526518954613No Hit
CCTCGG432300.17074627695701777No Hit
TAAGTC427080.1686845245496256No Hit
ATCCGG420360.16603031455390235No Hit
TTAGTA415680.16418184687830936No Hit
TGCATA415380.16406335536064315No Hit
AACCTT405130.1600148951737141No Hit
CACATT395240.15610862480798451No Hit
CATACG386430.15262892390585328No Hit
TCTCTG378400.1494573009496542No Hit
GAGAAG352750.13932627618919274No Hit
AAGAGG345340.13639953570283717No Hit
TAATGA332690.1314031433745784No Hit
AGGCTT323590.12780890067203649No Hit
CTCGTC323480.12776545378222556No Hit
GCATAA317140.12526133304221282No Hit
TCAAGC316040.12482686414410338No Hit
CAGCCT306910.12122077228979486No Hit
CCGCTC299260.11819923858930634No Hit
TCGTGG293730.11601504494699241No Hit
GTACGC289190.11422187331297702No Hit
GTTACA288020.11375975639407877No Hit
TTGAAT276490.10920573239844053No Hit
CTGTTC276330.10914253692235187No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)