FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25485032
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATAA4701661.8448711384784606No Hit
ATCCGG4431961.7390443143253658No Hit
TTCTGT3858141.5138846990657104No Hit
AACCTT3647371.4311812518030191No Hit
ACAGTG3647211.4311184698532062No Hit
TTACGT3417521.3409910570251589No Hit
ATCAGC3383241.32754002427778No Hit
CTTCAC3346591.3131590338988No Hit
GTACTT3011561.1816975548627917No Hit
CTGTAT2999051.1767887911618082No Hit
CGTACT2995501.175395816650338No Hit
TAGCTT2947651.1566200897844663No Hit
TTCACA2891541.1346032447595122No Hit
CCGGAA2850491.1184957507606819No Hit
ATTCAT2826351.1090235240826851No Hit
CTATGC2744581.0769380238565132No Hit
TAATGA2719891.0672499842260352No Hit
CAAGGA2701891.0601870148721022No Hit
GGCTAC2673601.0490863813708375No Hit
TCTTCA2638621.035360677593028No Hit
GAGTCG2636831.034658304529498No Hit
GACCAT2623381.0293806968733648No Hit
TAGAGC2600681.0204735077436826No Hit
TCAAGC2553121.0018115731618464No Hit
ATGTGA2544620.9984762820780448No Hit
CTAGTT2472790.9702911104839892No Hit
ATTGAA2425930.9519038469325838No Hit
GGCAGG2395300.9398850274153079No Hit
GTCTGC2395220.9398536364404017No Hit
ACTTGA2389020.937420835885158No Hit
GCTCAA2366850.9287216119642305No Hit
TGACAC2316770.9090708616728439No Hit
CTTGTA2303740.9039580566349691No Hit
AGTTCC2203750.8647232618738718No Hit
TGGCAT2202250.8641346810943773No Hit
ATACCT2193820.8608268571136187No Hit
AACTCT2163810.8490513176518671No Hit
CAGATC2120630.8321080389461548No Hit
ACTAAT2042600.8014900667968555No Hit
TCAATG2010660.788957220065488No Hit
CCTCGG2005190.786810862156265No Hit
ACGAAC1935150.7593280636257392No Hit
TATAAG1920830.7537090791174993No Hit
CTACCG1885190.7397243997967121No Hit
AACTAG1878950.7372759037540153No Hit
CTCGTC1841470.7225692320103816No Hit
CCAGCG1839620.7218433157156718No Hit
AGAACG1827630.7171385933515798No Hit
CACCTA1826160.7165617841876754No Hit
CTGTTC1790250.7024711603265792No Hit
TTAGTA1786410.7009643935310734No Hit
AGGACT1786390.7009565457873469No Hit
TGCATA1786180.7008741444782176No Hit
CATTAA1783730.6999127958717102No Hit
TATGGT1770790.694835305680605No Hit
TAAGTC1760300.6907191640960074No Hit
GCATAA1739550.6825771299796681No Hit
AGGTCA1725910.6772249687581322No Hit
GAGAAG1725750.6771621868083195No Hit
AATGTA1725650.6771229480896865No Hit
CAAGCT1573490.6174173138177734No Hit
GTTACA1551080.608623916972127No Hit
ACGTAA1532980.6015217088995611No Hit
ACCAGG1457530.5719160956909923No Hit
CCGACT1455240.5710175290342975No Hit
ACGCTA1435050.5630952317423027No Hit
GAATAC1433860.5626282909905704No Hit
CCATGA1417770.5563147811625271No Hit
GCGCAG1410360.5534071921118247No Hit
GTACGC1389400.5451827566863562No Hit
TACAGT1376010.5399286922614026No Hit
CGAATT1331600.5225027773165049No Hit
GATCAG1304750.5119671813635549No Hit
ATTGCT1298600.5095540001676278No Hit
TGACCA1279510.5020633287805956No Hit
TACGGC1269530.4981473046610261No Hit
GGTGGT1199240.470566409333918No Hit
AAGCAT1195500.4690988812570453No Hit
TGCGAC1186690.46564195014548143No Hit
GGAGGC1175110.46109810652778466No Hit
CAGTAG1172440.4600504327402845No Hit
TGTGTG1148990.4508489532208553No Hit
CCTGAC1128450.4427893204136451No Hit
CGTTGC1111090.43597747885896315No Hit
CCTGCT1064730.4177864089007226No Hit
GGCGTT1064620.4177432463102263No Hit
GGTAAT1052550.413007132971228No Hit
GCGTAT1044430.4098209490182316No Hit
ATGCCG1041670.4087379603839618No Hit
CATAAC1033480.4055243093279224No Hit
TCTCTG1027890.4033308649563399No Hit
ATTCGA1024720.40208699757567506No Hit
CAGCCT1022840.40134930966537535No Hit
CCACTT984910.3864660636878933No Hit
AGTAGG977430.38353100753414787No Hit
CAACGG971140.3810628921321346No Hit
TTGAAT970810.3809334043606459No Hit
CATACG970370.38076075399866083No Hit
CGTCAG960250.37678979567300525No Hit
TCATGT949120.37242252628915673No Hit
ATCCAC928730.36442175155989603No Hit
TTAGGC926810.36366836816214315No Hit
GACCGA925120.36300523381724614No Hit
AGGCTT921680.3616554218962723No Hit
CTCCGC903520.35452967059252666No Hit
GACACG888220.34852614664168363No Hit
GGCCGT874670.3432093002669174No Hit
GTCTCA872330.3422911142509062No Hit
ACAGCC860370.33759816350240407No Hit
GCACAT852570.33453754344903314No Hit
AGTCAA844840.3315043904987053No Hit
GACTAA831960.32645044353877994No Hit
CGTGAA814020.3194110174160268No Hit
GAGTGA779260.30577163881920966No Hit
CGAGAG774110.3037508448096122No Hit
AGCCTA771610.30276987684378814No Hit
TTAGCG764790.30009379623302024No Hit
AATCGG754880.29620523921649383No Hit
TGTGAT753440.29564020166817917No Hit
TTCGTT747860.29345068116845996No Hit
TATTCT743900.29189682791059474No Hit
GTTGGC731310.286956673234705No Hit
GGATTC724230.2841785719554914No Hit
TCGTGG721080.2829425523185531No Hit
AGGTGT717670.28160451201316916No Hit
AACAAT699790.27458862912159576No Hit
GTAAGA698630.2741334599854534No Hit
AATTGC691890.27148877034959185No Hit
GCTTCT689370.2704999546400413No Hit
AGAGGT670000.26289941484083673No Hit
GGCCAG669820.26282878514729746No Hit
ACCGCT667680.2619890765685521No Hit
CGCTTG664680.26081191500956324No Hit
GCGGCT661430.25953665665399206No Hit
CGAGCC652570.2560601061831117No Hit
CTATTA652350.2559737810021192No Hit
TTCTAC647970.2542551251259955No Hit
TCCTCC639850.2510689411729991No Hit
TGCGGA636040.24957394599308333No Hit
GAGATA630780.2475099893929896No Hit
CAGGAC630570.24742758808386037No Hit
ACACGA615510.24151823705773648No Hit
AAGGCG615260.24142014026115408No Hit
AAGAGG605820.2377160052222026No Hit
GGTACC603890.23695869795258645No Hit
GATTCA598910.235004609764665No Hit
AGATGC595380.2336194829969215No Hit
AACATA591180.23197145681433715No Hit
ACCTGC583130.2288127399643838No Hit
GCCTGG577420.22657220913044177No Hit
GCTGTC576170.22608172514752972No Hit
GAGCCA569650.2235233606926607No Hit
TACTCG554470.21756692320417723No Hit
CCTTAT546800.21455731348502916No Hit
AAGCGC508710.1996112855577344No Hit
CACATT502080.19700975851236913No Hit
ACTAGC493370.19359206611943827No Hit
GATCCT488670.1917478463436891No Hit
CTGGAG451290.1770804133186884No Hit
CGTCTC441510.17324286663638486No Hit
GTCGAG433620.17014693173624423No Hit
AAGTTG432540.16972315357500825No Hit
GGACTA430790.16903647599893146No Hit
GAAGAT417810.16394329032037316No Hit
TCCAGA410210.16096114770426814No Hit
CCGCTC409840.1608159644453262No Hit
TCCACG404420.15868922589541973No Hit
CTGAGG402330.15786913667599084No Hit
AGACGG398300.1562878163150825No Hit
GAGCAC394730.15488699405988582No Hit
TCTCGC369010.14479479562748832No Hit
TATTGG357960.14045891721854614No Hit
CTTAGC322470.1265330959757084No Hit
GGATCG314450.12338615074134496No Hit
CATGAG302990.11888939358600766No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)