FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23263878
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGG4813262.068984371393282No Hit
AACTAG4146331.782303879000741No Hit
CAAGCT3875121.665724003538877No Hit
ACAGCC3711681.5954691646852686No Hit
CATTAA3649381.5686894506582265No Hit
GACCAT3354971.4421370332151846No Hit
CTTCAC3278801.4093952865468087No Hit
ATCCAC3256931.3999944463257588No Hit
TGATAA2991331.2858260346791708No Hit
ACGTAA2858681.2288063064979966No Hit
AATGTA2788261.1985362027775421No Hit
AAGTTG2673001.1489915825727766No Hit
CCATGA2488021.0694777543107816No Hit
ACCTGC2475751.0642034831853915No Hit
TACGGC2454741.0551723147791612No Hit
GCTTCT2425421.0425690849995002No Hit
CTAGTT2382641.024180061466966No Hit
GAGTGA2193580.9429124413393158No Hit
AAGAGG2158010.9276226431380014No Hit
GCACAT2152700.9253401346069645No Hit
TCAATG2145970.922447237730528No Hit
GTTACA2073380.8912443574540754No Hit
CACCTA2030930.8729971847342046No Hit
TATTCT1977290.8499399799122055No Hit
TATGGT1938960.8334637930958889No Hit
GAGAAG1934490.8315423593607223No Hit
AGGCTT1928650.8290320298275292No Hit
CGAGCC1879940.8080939901765304No Hit
TTAGTA1864920.8016376289456126No Hit
TTCTGT1852960.7964966116139364No Hit
AGTAGG1831160.7871258609592089No Hit
CCTGCT1827620.7856041886051844No Hit
AGATGC1818690.7817656196443259No Hit
TTCGTT1816780.7809446043346685No Hit
GATCCT1766400.7592887136014039No Hit
CAACGG1731860.7444416618759778No Hit
CATAAC1695890.7289799232956775No Hit
CAGATC1694780.7285027887439919No Hit
GTAAGA1687400.7253304887517034No Hit
AGAACG1678340.7214360391676744No Hit
GCATAA1673260.7192523963545545No Hit
TTGAAT1628960.7002099993818743No Hit
GGCCGT1618670.6957868331324639No Hit
GATCAG1548170.6654823413362123No Hit
AGGACT1524560.655333560466574No Hit
GAGTCG1512800.6502785133243907No Hit
ATGTGA1500050.6447979137442175No Hit
ACTTGA1499590.6446001823083839No Hit
AACTCT1497210.6435771370534182No Hit
GCGTAT1496560.6432977339375663No Hit
GAGATA1488530.6398460308294258No Hit
TCGTGG1462760.628768771913264No Hit
CCAGCG1461090.6280509208309981No Hit
CTGTAT1423460.6118756296779067No Hit
GTACTT1423050.6116993907894462No Hit
AGTGCA1388600.596891025649292No Hit
GACCGA1361360.5851818858403574No Hit
TGACAC1354470.5822202128123265No Hit
CGTACT1332010.5725657605322724No Hit
GAGCCA1323960.5691054604051827No Hit
GGTAAT1321400.5680050419796734No Hit
TGACCA1297070.5575467684278606No Hit
CGAATT1295870.5570309472909031No Hit
CAGTCC1259410.5413585817463451No Hit
GATTCA1259100.5412253279526311No Hit
AACCTT1235870.5312398904430293No Hit
CTGGAG1234400.5306080095502564No Hit
ATTCAT1230420.5288972027793475No Hit
ATACCT1227680.5277194111832946No Hit
CTGAGG1212930.5213791097081922No Hit
CGTTGC1207280.5189504518550174No Hit
TGCGAC1201810.5165991671723863No Hit
CTCGTC1180770.5075551032377319No Hit
TTCACA1166010.501210503253155No Hit
AATTGC1152260.4953000527255172No Hit
CAGCCT1146500.4928241112681213No Hit
GAGCAC1131590.48641503364142474No Hit
TAGCTT1117490.48035413528217435No Hit
TTACGT1116640.4799887619768295No Hit
TCTTCA1087780.46758326363300223No Hit
ATTGCT1083780.46586385984314393No Hit
CAGGAC1080160.46430779941332223No Hit
ACCAGG1076770.46285060470141737No Hit
ACAGTG1065980.45821251297827476No Hit
ATTGAA1057530.4545802724721991No Hit
TCAAGC1046790.4499636732964297No Hit
TAAGTC1030580.4429957894380292No Hit
ACCGCT1029510.44253584892424214No Hit
CAAGGA1016710.43703375679669576No Hit
AGTCAA1004830.4319271275408167No Hit
CTTGTA998580.4292405591191632No Hit
TCGGAC998330.4291330963822971No Hit
TATTGG998020.4289998425885831No Hit
TACAGT965560.41504688083388336No Hit
GTCTCA965450.4149995972296623No Hit
ACTAGC960300.41278586485021973No Hit
ATCAGC957880.4117456255573555No Hit
CGTCAG950090.4083970866766065No Hit
TTCCAT941720.40479923424632813No Hit
GGATTC931760.4005179188095811No Hit
TACGAA921410.39606896150332294No Hit
GGCTAC909200.39082048143478054No Hit
ACACGA908880.3906829291315919No Hit
TCCTCC900360.38702059905919384No Hit
CCGGAA900100.386908837812853No Hit
CACATT889810.3824856715634427No Hit
CCGCTC889150.3822019699381161No Hit
CCTTAT884920.380383700430341No Hit
AATCGG884390.3801558794281848No Hit
TTCTAC876200.37663540016845No Hit
ATTCGA875290.3762442358062572No Hit
GCCAAT857970.368799217396171No Hit
TGTGAT832420.35781652568845146No Hit
TGCGGA812470.3492409992865334No Hit
AAGCAT802740.3450585495677032No Hit
ACTAAT798300.3431500113609605No Hit
TCATGT790810.3399304277644509No Hit
TAGAGC789570.33939741258959494No Hit
ATCCGG778010.3344283356369046No Hit
GGCAGG775740.33345257398616No Hit
GACACG760920.3270821829447352No Hit
CCTGAC759900.32664373497832133No Hit
GTCGAG758600.3260849287466174No Hit
CCGTCG757700.3256980628938993No Hit
CGCTTG739470.3178618801216203No Hit
GGTACC737660.31708384990670946No Hit
GCCTGG736980.3167915512624336No Hit
TCCACG733850.3154461177968695No Hit
AGTTCC724520.3114356084570251No Hit
TCTCGC721250.31002999585881597No Hit
GTACGC717680.3084954279763675No Hit
TACTCG714050.30693506903707113No Hit
AGGTCA703650.30246461918343964No Hit
GGATCG702870.30212933544441734No Hit
AGTTAG699750.3007882004883279No Hit
GACTTG696890.2995588267785792No Hit
TAATGA686280.2949981082259802No Hit
TGTGTG652150.2803272953890147No Hit
TGCATA641240.2756376215521763No Hit
AACATA629360.2705309922962973No Hit
CGAGAG623500.2680120657441549No Hit
GGTGGT619820.26643021425748536No Hit
CTCCGC616960.2652008405477367No Hit
CTATGC608420.2615299134563894No Hit
TATAAG585250.25157026700363544No Hit
CGTGAA583770.25093408760138786No Hit
CCACTT575370.24732333964268555No Hit
AAGCGC575270.2472803545479391No Hit
ACGAAC565940.2432698452080947No Hit
TCCAGA540210.2322097803298315No Hit
CTATTA527130.226587329936995No Hit
GAAGAT526290.2262262551411248No Hit
CTTAGC526230.2262004640842769No Hit
GGAGGC522000.22438219457650183No Hit
GACTAA511970.22007078957343226No Hit
CCGACT509030.21880702778788644No Hit
GTCTGC508510.21858350529520487No Hit
GCTGTC507680.21822672900880927No Hit
CGTCTC503750.21653741478527355No Hit
AGGTGT498130.2141216524605227No Hit
AGCCTA495650.21305562211081058No Hit
CATACG491410.21123305409356083No Hit
GGACTA486180.20898493363832116No Hit
GGCGTT475360.2043339463867546No Hit
GCCATA466100.20035352661323275No Hit
ACGCTA465350.2000311384026343No Hit
GAATAC442710.19029931295203664No Hit
CATGAG442610.19025632785729016No Hit
GTGGTG442020.1900027157982861No Hit
ATGGCC428390.18414384738434408No Hit
GTTGGC428080.18401059359063007No Hit
GGCCAG420650.18081680105096837No Hit
AGAGGT415880.1787664120315624No Hit
GCGCAG415200.1784741133872865No Hit
GCTCAA408470.17558121651085No Hit
AAGGCG402030.17281297640917823No Hit
TCTCTG400100.1719833640805716No Hit
TTAGCG387930.16675207804992787No Hit
CAGTAG379340.1630596584112073No Hit
GAACCG364610.15672795395505426No Hit
TTAGGC363400.15620783430862215No Hit
ATGCCG352120.1513591156212219No Hit
GCGGCT341620.14684568067284395No Hit
CGACAA334650.14384961956901596No Hit
AACAAT315380.13556639181137384No Hit
CGAAGC299110.1285727168961254No Hit
CTACCG275190.11829068223277305No Hit
AGACGG269730.11594369605961653No Hit
TGGCAT249590.10728649797768024No Hit
CTGTTC238600.1025624360650447No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)