FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22684597
Sequences flagged as poor quality0
Sequence length6
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAC19278018.49828189586088No Hit
ATACCT6280202.7684864756468897No Hit
TGATAA6070582.6760801613535388No Hit
ATCAGC5678782.5033638464020322No Hit
CTAGTT5413932.386610615123557No Hit
GACCAT4896962.1587158899053835No Hit
ATTCAT4716262.079058314326677No Hit
TTCTGT4533981.998704230892883No Hit
CTGTAT4383211.932240630062769No Hit
TCATGT3739411.6484357205023301No Hit
ATTGAA3696241.629405186259205No Hit
TATAAG3452861.5221165269103083No Hit
ATTCGA3439921.516412215742691No Hit
GATTCA3375301.4879259261251148No Hit
CTGAGG3337481.4712538203786474No Hit
AATTGC3086321.360535521085078No Hit
ATTGCT2985141.315932568694079No Hit
TGTGAT2823691.2447609274257772No Hit
GTCTGC2744891.2100237002226666No Hit
GTGGTG2740121.2079209518247118No Hit
ACTAAT2668291.1762562940836023No Hit
GGTAAT2402351.059022560550668No Hit
CGTACT2360471.0405606941132788No Hit
TGCGAC2326891.025757698053882No Hit
CTTCAC2230550.9832883520037848No Hit
GGCCGT2125340.9369088637545555No Hit
GTCGAG2119210.9342065896079176No Hit
TCTCGC2018000.8895904123842271No Hit
ACTTGA1999740.8815408975526432No Hit
CTCCGC1959370.8637446810273949No Hit
AGAACG1884120.8305723923594499No Hit
AGGTGT1861660.8206714009510505No Hit
AAGCGC1858060.8190844210280659No Hit
GCCTGG1831580.8074113020390004No Hit
TGACCA1809420.7976425589575163No Hit
ACAGTG1778480.7840033481749753No Hit
CCAGCG1760980.7762888624382439No Hit
TACTCG1747920.7705316519398603No Hit
TGACAC1708620.7532071211139435No Hit
AGTGCA1701520.7500772440436125No Hit
AGTTAG1673790.7378530903590661No Hit
TCCAGA1663050.7331186002554949No Hit
TTCACA1647450.7262416872558944No Hit
CAGATC1641310.7235350048316926No Hit
CAGTCC1626240.716891730542976No Hit
TCTTCA1609560.70953872356648No Hit
GATCCT1600520.7055536406487627No Hit
TATTGG1586310.6992894782305368No Hit
GAGCCA1578960.6960493942211097No Hit
CGAATT1569160.69172928220854No Hit
GCCAAT1544800.6809907180630099No Hit
GATCAG1544320.6807791207399452No Hit
AATCGG1507100.6643715116473086No Hit
CGCTTG1486150.6551361701510501No Hit
CTTGTA1441150.6352989211137408No Hit
CGAGCC1388650.6121554639035466No Hit
CTTAGC1314660.5795386182086462No Hit
AGAGGT1306980.5761530610396121No Hit
TTCCAT1305670.5755755766787481No Hit
ATGTGA1298010.5721988360648417No Hit
GCTCAA1267360.5586874653316521No Hit
CTATTA1241750.5473978664906411No Hit
GACTTG1209950.5333795438376093No Hit
CCACTT1186530.5230553577830808No Hit
CCGTCG1164010.5131279167092984No Hit
GGTACC1146750.5055192296340992No Hit
AGTTCC1140510.502768464434259No Hit
TACAGT1131930.4989861622844788No Hit
GACACG1092750.48171453078932813No Hit
GGCTAC1090390.4806741772842603No Hit
TACGAA1079160.4757236815800607No Hit
CCGCTC1064580.4692964128919725No Hit
TCGGAC1056420.4656992583998737No Hit
GTACTT1037050.457160424758703No Hit
TTAGCG1026570.4525405498717918No Hit
AACTCT972240.4285903778674137No Hit
GCGGCT962220.4241732837484395No Hit
CGTCTC959850.42312852196580786No Hit
AGTCAA954310.42068633619543694No Hit
TTAGGC948900.4183014580333959No Hit
CCGGAA910670.4014486129068107No Hit
GTCTCA893270.39377820994571777No Hit
TCCTCC882750.38914070194855127No Hit
CTACCG882330.3889555542908697No Hit
TAAGTC812450.35815051067471027No Hit
CCTGAC807000.35574799940241386No Hit
ATCCAC806780.35565101729600923No Hit
GAACCG766250.33778426832973935No Hit
AGGTCA759290.33471610714530214No Hit
TATTCT754210.3324767021428682No Hit
GCTGTC752650.33178901084290807No Hit
GGCAGG740240.3263183383861746No Hit
GAAGAT736970.32487683162279674No Hit
GCGTAT717750.31640412214508373No Hit
GCGCAG708740.31243226406005803No Hit
CGAGAG703500.31012232661660244No Hit
TAGAGC697630.3075346676866245No Hit
CATGAG687600.3031131652900865No Hit
CGACAA673740.2970032925865952No Hit
TGCGGA666570.2938425575733173No Hit
GACTAA638990.28168452805222854No Hit
TCAAGC633390.2792158926164745No Hit
ACACGA609980.2688961148395098No Hit
CAAGGA608730.268345080144029No Hit
CGAAGC602610.26564721427495497No Hit
CAGGAC592660.2612609780989276No Hit
AACATA563250.24829623378365503No Hit
ACCAGG551630.2431738152544654No Hit
GGATTC549360.24217313624747225No Hit
GCCATA547960.2415559773885337No Hit
GTAAGA505850.22299272056717606No Hit
GGATCG490660.21629654694769318No Hit
GTTGGC489000.21556477287209466No Hit
CACATT470950.20760783186935172No Hit
TCCACG448770.19783027223274013No Hit
ACCGCT406790.17932432301971246No Hit
AAGCAT376030.16576446123332056No Hit
CATACG231840.10220150704021766No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)