FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22578875
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTC16694257.393747474132348No Hit
GCGTAT11404315.050876095465341No Hit
AGTTCC10780334.774520431155229No Hit
GACTAA10405114.60833854653963No Hit
TCCTCC7455523.3019891380770745No Hit
GCGGCT7055793.124951973913669No Hit
TTAGCG5343492.366588237899364No Hit
TGCGGA5152402.2819560319103585No Hit
CTTCAC5013092.2202567665572355No Hit
GGCAGG4672512.069416656055716No Hit
AGGTGT4519812.001787068664847No Hit
TACAGT4336461.920582845690939No Hit
AACTCT4153001.839329904612165No Hit
GACACG3671531.6260907596149055No Hit
GTTGGC3287401.4559627085051847No Hit
ATTGCT2985781.3223776649633785No Hit
AATTGC2884661.2775924398359086No Hit
GATTCA2710861.20061783414807No Hit
ATTCGA2693361.192867226555796No Hit
GTACTT2675821.1850989032890258No Hit
TCGGAC2655801.1762322082034644No Hit
TGACAC2536921.1235812235994929No Hit
AACATA2398091.062094546340329No Hit
GGTAAT2395921.061133470998887No Hit
CCTGAC2387471.057391034761475No Hit
CAAGGA2360061.0452513688126623No Hit
TACTCG2321251.0280627356323113No Hit
ACCAGG2258471.000257984509857No Hit
CGACAA2102750.9312908636944932No Hit
AGTGCA2028850.8985611550619771No Hit
TAGAGC1940000.8592102130863474No Hit
CTCCGC1875140.8304842468900687No Hit
CCGTCG1832080.8114133232944511No Hit
GTCTCA1827830.8095310328791846No Hit
TGACCA1813890.8033571203171106No Hit
TTCCAT1807330.8004517496996639No Hit
AGGTCA1782560.7894813182676285No Hit
CGTACT1756640.778001561193815No Hit
ATACCT1744450.7726027093909683No Hit
TCTCGC1700590.7531774723054182No Hit
TTCTAC1694740.7505865549102867No Hit
ACAGTG1694470.7504669741074345No Hit
TTAGGC1690840.7488592766468657No Hit
GCCTGG1683150.7454534382248894No Hit
TACGAA1644080.7281496531603103No Hit
GTCGAG1580830.7001367428625208No Hit
CTGAGG1535910.6802420404028102No Hit
GCCAAT1506560.667243164240911No Hit
CATGAG1464880.648783431415427No Hit
CAGTCC1446890.6408158068105696No Hit
GCTGTC1439640.6376048408080562No Hit
TGCGAC1425430.6313113474431299No Hit
TGATAA1421050.6293714810857494No Hit
ACTTGA1404710.6221346280538778No Hit
GGCCGT1372040.6076653509087587No Hit
TGTGAT1285870.5695013591244028No Hit
TTCTGT1284740.5690008913198731No Hit
GAACCG1200760.5318068327142075No Hit
CTACCG1195180.5293354961219281No Hit
ATCAGC1152120.5102645725263105No Hit
ATTCAT1128110.499630738909711No Hit
GATCAG1124760.4981470511706185No Hit
TCATGT1120300.49617175346424475No Hit
CGCTTG1110860.4919908542830411No Hit
CCAGCG1082740.4795367351119132No Hit
TTCACA1073610.4754931324080584No Hit
AAGCGC1060280.46958938388205784No Hit
GACTTG1009030.4468911759332562No Hit
TATAAG982810.4352785513007181No Hit
AGAACG975190.43190371530911087No Hit
GGCTAC920310.4075978098997403No Hit
AGTTAG889430.3939213091883453No Hit
CGAAGC860790.3812368862487613No Hit
TAGCTT817200.3619312299660634No Hit
CTTAGC776270.34380366603739115No Hit
CTAGTT769560.34083186164058216No Hit
ATGTGA768100.34018523952145535No Hit
CTTGTA757770.33561016658270176No Hit
GACCAT750900.33256749948790626No Hit
CTGTAT743370.3292325237639165No Hit
TCCAGA709390.3141830582790329No Hit
TCTTCA707690.31343014211292636No Hit
GTGGTG686210.30391682490823835No Hit
GCCATA639070.28303890251396496No Hit
TATTGG602160.26669176387220356No Hit
ATTGAA578750.25632366537305334No Hit
CGAGCC567620.2513942789443673No Hit
GAAGAT523340.23178302727660258No Hit
CCGGAA515480.22830189723801564No Hit
GGTACC406950.1802348434100459No Hit
CCACTT398750.1766031301382376No Hit
GATCCT386670.17125299644025666No Hit
CTATTA361930.16029585176409364No Hit
CAGATC330730.1464776256567256No Hit
ACACGA281490.12466963035137933No Hit
GAGCCA272940.12088290492772559No Hit
ACTAAT270150.11964723663158594No Hit
GCGCAG263040.1164982754898107No Hit
GTCTGC243910.10802575416179946No Hit
GCTCAA227950.1009572000376458No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)