FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21799570
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAC4362602.0012321343953117No Hit
TACTCG3125701.4338356215283146No Hit
GGTAAT2802781.2857042593041972No Hit
AGAACG2728721.251731112127441No Hit
GCTTCT2620581.2021246290637844No Hit
TCTTCA2604861.1949134776511647No Hit
GACTTG2430841.1150862150033236No Hit
ACTAGC2339191.0730441013286043No Hit
TCAATG2288061.0495895102518078No Hit
TGATAA2265861.039405823142383No Hit
AGCCTA2176400.9983683164392693No Hit
CGAGCC2145110.9840148223107152No Hit
ATTCAT2085860.9568353871200213No Hit
CCAGCG2076710.9526380566222178No Hit
TTCACA2063400.9465324316030086No Hit
TGTGAT2062690.9462067371053648No Hit
ATTCGA2046060.9385781462661879No Hit
CGTACT2001110.9179584734928258No Hit
GATCAG1959960.8990819543688248No Hit
ACTTGA1958380.8983571694304061No Hit
CATTAA1942960.8912836354111572No Hit
GGCCGT1914430.8781962213016128No Hit
TGCGAC1909720.8760356282256944No Hit
AAGCGC1904340.873567689637915No Hit
AGTGCA1901320.8721823412113175No Hit
AAGTTG1864380.8552370528409505No Hit
ACACGA1825330.8373238554705438No Hit
CCGGAA1808570.8296356304275727No Hit
GGTGGT1785000.8188234905550889No Hit
AACAAT1768980.8114747217490987No Hit
GTCGAG1760280.8074838173413512No Hit
ACAGTG1745840.8008598334737795No Hit
TATTGG1745460.8006855181088435No Hit
CCTGAC1734530.7956716577437078No Hit
CTGTAT1719150.7886164727102416No Hit
CTTCAC1703470.7814236702834046No Hit
GGCCAG1687790.7742308678565678No Hit
CTGAGG1664680.7636297413205857No Hit
AGAGGT1656920.7600700380787327No Hit
CAGTCC1623680.7448220308932699No Hit
CAGGAC1616060.7413265491016565No Hit
CAGATC1612820.7398402812532541No Hit
AATGTA1608630.7379182249925114No Hit
TCTCGC1603170.7354135884331664No Hit
GATCCT1597430.7327805089733421No Hit
TTCTAC1593750.7310924022813293No Hit
GACCAT1551870.7118810141667933No Hit
CATAAC1505230.6904860967441101No Hit
TAGCTT1495320.6859401355164345No Hit
ATGCCG1468230.673513284895069No Hit
ATTGAA1468130.6734674124306121No Hit
TACAGT1457960.6688021827953486No Hit
AGGTGT1456140.6679673039422338No Hit
GATTCA1448580.6644993456292946No Hit
GGTACC1448350.6643938389610438No Hit
GAATAC1433720.657682697411004No Hit
GGCAGG1432280.657022133922825No Hit
GTCTCA1411020.647269647979295No Hit
AGGTCA1401010.6426778142871625No Hit
TCATGT1400580.642480562689998No Hit
CTCCGC1394680.6397740872870429No Hit
GGCGTT1358510.6231820168929938No Hit
ATACCT1344420.6167185866510211No Hit
GTACTT1343710.6163928921533773No Hit
GAAGAT1335810.6127689674612847No Hit
AGGACT1327690.6090441233473871No Hit
ACTAAT1324930.6077780433283776No Hit
CTAGTT1322630.6067229766458696No Hit
GAGAAG1321950.606411043887563No Hit
GGAGGC1288930.5912639561239051No Hit
GCGCAG1281120.5876813166498238No Hit
GAGCCA1273280.5840849154364054No Hit
TTACGT1268880.5820665270003033No Hit
AGTTAG1233460.5658185000896807No Hit
CAGCCT1216210.557905499970871No Hit
GCACAT1198920.5499741508662785No Hit
CCGTCG1181030.5417675669749449No Hit
AACTCT1149290.5272076467563351No Hit
ATCAGC1135790.521014864054658No Hit
TTCCAT1118550.5131064511822939No Hit
TCGGAC1117000.5123954279832126No Hit
TCTCTG1087680.4989456214044589No Hit
AATCGG1075430.4933262445084926No Hit
TAAGTC1072370.49192254709611244No Hit
AATTGC1071810.49166566129515393No Hit
TTCGTT1063510.48785824674523404No Hit
CCATGA1055690.4842710200247069No Hit
GGCTAC1049350.4813627057781415No Hit
CACCTA1048940.48117462867386834No Hit
GTCTGC1047080.48032140083497055No Hit
GCCAAT1041380.4777066703609291No Hit
GTACGC1026280.47077992822794207No Hit
TCCACG1016330.4662156180144838No Hit
CCACTT1015850.4659954301850908No Hit
GCGTAT981200.45010062125078615No Hit
CAAGGA967590.4438573788382065No Hit
CTTGTA953340.43732055265310277No Hit
CAGTAG934230.4285543246953954No Hit
CTACCG924480.4240817594108508No Hit
CGTTGC917940.42108170023537167No Hit
ATGTGA899020.4124026299601322No Hit
CGAATT890040.4082832826519055No Hit
TCCAGA862210.39551697579355927No Hit
TGACCA856510.39290224531951773No Hit
GCGGCT855500.3924389334285034No Hit
CCTTAT845220.3877232440823374No Hit
CTATGC844630.3874525965420419No Hit
ACGTAA841030.3858011878215946No Hit
AACCTT837500.3841818898262672No Hit
CCTGCT832180.38174147471716185No Hit
GACACG821220.37671385261268914No Hit
AACATA820480.3763743963757083No Hit
ATGGCC814460.3736128740154049No Hit
ACGCTA810590.3718376096409241No Hit
AGTTCC799850.3669109069582565No Hit
AGGCTT797020.36561271621412716No Hit
GTAAGA793980.3642181932946384No Hit
CCGCTC790260.3625117376168429No Hit
GCTCAA786440.36075940947459056No Hit
ACCAGG784670.359947466853704No Hit
GCTGTC781970.35870891031336855No Hit
CAAGCT780250.35791990392471046No Hit
ACCTGC775670.3558189450525859No Hit
ATTGCT762840.3499335078627698No Hit
AGATGC762650.34984635018030175No Hit
GAGATA754880.3462820596920031No Hit
GTTGGC746790.3425709773174425No Hit
CGTGAA741950.3403507500377301No Hit
TGACAC728730.3342864102365322No Hit
CCGACT714850.32791931216991893No Hit
TTAGTA711460.32636423562483113No Hit
TGCGGA711140.32621744373856915No Hit
CTATTA696410.3194604297240725No Hit
AAGGCG691170.3170567125865327No Hit
ATCCAC683780.31366673746317014No Hit
GTGGTG678000.3110153090175632No Hit
GCCATA667180.3060519083633301No Hit
ATCCGG638940.29309752440071063No Hit
TTCTGT613620.28148261640023176No Hit
TACGGC612510.2809734320447605No Hit
CGACAA595970.27338612642359456No Hit
ACAGCC594120.27253748583114257No Hit
AGTAGG589840.2705741443523886No Hit
CTTAGC587670.26957871187367455No Hit
CTCGTC581720.26684930023849096No Hit
GCCTGG570950.2619088358164863No Hit
GGATTC564320.258867491422996No Hit
CAACGG559470.25664267689683784No Hit
AAGCAT547830.25130312203405847No Hit
TCAAGC545830.25038567274492113No Hit
GAGTGA545460.2502159446264307No Hit
TCCTCC538110.24684431848885094No Hit
CGTCAG527130.24180752189148685No Hit
CGAGAG524720.24070199549807636No Hit
CCTCGG520790.23889920764492145No Hit
TAGAGC517970.23760560414723778No Hit
TTGAAT515480.23646337978226176No Hit
GGATCG513370.23549547078222186No Hit
CATACG510320.23409636061628739No Hit
TCGTGG506010.23211925739819636No Hit
CGTCTC491870.2256328909239953No Hit
TACGAA488090.2238989117675257No Hit
CGCTTG488060.22388515002818862No Hit
CATGAG479520.21996764156357212No Hit
CGAAGC458980.2105454373641315No Hit
GAACCG451480.20710500252986644No Hit
AGTCAA450830.20680683151089677No Hit
AAGAGG429030.19680663425929962No Hit
TTAGGC421740.19346253160039395No Hit
AGACGG403640.1851596155337009No Hit
GAGTCG401750.18429262595546608No Hit
TTAGCG393520.18051732213066587No Hit
GGACTA374090.1716043022866965No Hit
CTGTTC362580.16632438162771102No Hit
ACGAAC362290.1661913514807861No Hit
TATAAG348990.16009031370802268No Hit
TATTCT324240.14873687875494793No Hit
CTGGAG318910.14629187639939686No Hit
TGCATA315160.14457165898226432No Hit
GACTAA302060.13856236613841466No Hit
ACCGCT290340.13318611330406976No Hit
CACATT287720.13198425473529984No Hit
TGGCAT286430.13139249994380622No Hit
AACTAG276950.12704379031329519No Hit
TAATGA264700.12142441341732887No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)