FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21366097
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGC4450472.082958810867516No Hit
TCAATG3577241.6742599268364269No Hit
CAGTAG3435841.6080803152770484No Hit
GGCGTT3369041.5768158311740326No Hit
TACTCG3053981.429357921570795No Hit
CATTAA3014831.4110345001241922No Hit
AGGACT2777951.300167269670263No Hit
GGCCAG2629041.2304727438052911No Hit
AATGTA2511921.1756569297612005No Hit
TTCACA2486721.1638625435426975No Hit
CGTACT2373231.1107456827515105No Hit
CTCCGC2317701.0847559102628805No Hit
AAGTTG2244931.0506972799009573No Hit
AGTGCA2242351.049489759407158No Hit
TGATAA2229141.0433070672664269No Hit
CTTCAC2170491.0158570374364584No Hit
AGAACG2164871.0132267020972525No Hit
TCTCGC2145451.0041375362098188No Hit
AGTTCC2140901.0020079942537001No Hit
AACAAT2051940.9603719387775878No Hit
GAGCAC1964140.9192787994924857No Hit
AGAGGT1940960.9084298362962595No Hit
AGCCTA1939660.9078213957373684No Hit
CCGACT1926190.901517015484859No Hit
ATGTGA1868560.8745443774780204No Hit
GGTGGT1842580.8623849269241828No Hit
GACCAT1835430.8590385038502821No Hit
CCTTAT1767410.8272030216843067No Hit
ACTAGC1737660.813279093509685No Hit
CCAGCG1729540.8094786801726117No Hit
TACAGT1676020.7844296503942672No Hit
GGTAAT1643220.7690782270622473No Hit
AGGTGT1622850.7595444315356239No Hit
AACTCT1586230.7424051290228627No Hit
CGAGCC1578230.7386608794296872No Hit
ACCAGG1577050.7381086026146937No Hit
ATGCCG1552030.7263984620120371No Hit
GAATAC1523360.7129800075324941No Hit
GCTTCT1503030.7034649332538367No Hit
GAGAAG1481690.6934771474640408No Hit
AGGTCA1460740.6836718938419122No Hit
ATTCAT1448170.6777887416686351No Hit
CCGCTC1354070.6337470058289074No Hit
CTACCG1336710.6256219842117163No Hit
ATTCGA1323390.619387808639079No Hit
TCCTCC1316880.6163409255326323No Hit
GTCGAG1305660.6110896154782036No Hit
TGTGAT1302370.6095497928330101No Hit
CGAATT1293580.6054357985925085No Hit
CCATGA1259450.5894618937656232No Hit
ACGCTA1253720.5867800749945111No Hit
CCGGAA1252540.5862277981795178No Hit
TCGGAC1238000.5794226245439212No Hit
GATTCA1231160.576221291141756No Hit
TGACAC1229850.5756081702708735No Hit
TTCGTT1222990.5723974762447255No Hit
ACTTGA1219790.5708997764074553No Hit
CTGAGG1210620.5666079303112778No Hit
AATTGC1209810.5662288250399686No Hit
TTCTGT1188440.5562269983141984No Hit
ATTGCT1177040.5508914426439232No Hit
ATACCT1160900.5433374190896915No Hit
AGTCAA1157180.5415963430288648No Hit
ATCCAC1153000.5396399726164306No Hit
CAGTCC1138910.53304541302045No Hit
GGCCGT1136730.5320251050063097No Hit
GGCAGG1132830.5301997833296367No Hit
TGACCA1110410.5197065238447621No Hit
CGTCTC1100830.5152227849569343No Hit
GACTAA1092450.5113006835080829No Hit
TCTTCA1091720.5109590207327056No Hit
GTAAGA1081520.5061851025014068No Hit
CATAAC1077280.5042006502170238No Hit
GAGTCG1076800.5039759952414332No Hit
ACCGCT1065230.498560874267303No Hit
TAAGTC1061440.4967870360225361No Hit
TTGAAT1049340.491123858512858No Hit
GCCTGG1043080.4881939832061981No Hit
TACGAA1031680.4828584275359229No Hit
TATAAG1028030.4811501136590366No Hit
CAGGAC1026610.4804855093562479No Hit
TGCGGA1024070.47929671011041464No Hit
CAGATC1017910.4764136379236695No Hit
GAGATA1002820.46935104712854203No Hit
GCACAT1000510.4682698950585125No Hit
CCTGAC991830.46420738424991703No Hit
AGACGG990060.4633789690274269No Hit
ATCCGG968370.45322737231792964No Hit
AACCTT947250.4433425533919461No Hit
ATGGCC939700.43980891783838666No Hit
CGTGAA927100.43391172472913514No Hit
TCATGT897550.42008140279434286No Hit
TTAGCG896880.41976782189091444No Hit
GATCAG883640.41357108881420873No Hit
GATCCT882220.4129064845114201No Hit
GAGCCA878580.41120285094652526No Hit
TAGAGC869930.4071543810739041No Hit
CCTGCT865620.40513716660558075No Hit
CGCTTG864640.40467849603041683No Hit
TGCGAC854980.40015731464665727No Hit
CTCGTC830030.3884799362279409No Hit
AGGCTT829850.3883956906120945No Hit
CAAGCT824770.386018092120428No Hit
TATTGG824700.3859853299364877No Hit
TTCTAC819840.3837106983086335No Hit
CTAGTT792290.37081643877213516No Hit
AAGCGC780940.3655042846618173No Hit
GGAGGC779400.364783516615131No Hit
TTACGT775100.3627709824587991No Hit
TCCAGA767510.35921862565727375No Hit
CAGCCT760850.3561015378709551No Hit
CCACTT758350.3549314598730877No Hit
ACAGTG748650.35039155724136234No Hit
CCGTCG746340.3493104051713329No Hit
ATCAGC726130.33985149463657305No Hit
ACACGA723830.3387750228785351No Hit
TTAGGC721920.3378810832881644No Hit
GCGTAT721850.33784832110422414No Hit
GAAGAT711190.33285910852131767No Hit
TATTCT711010.3327748629054712No Hit
ACCTGC708610.33165158802751854No Hit
AGATGC704750.3298449875988113No Hit
ATTGAA698270.3268121454283391No Hit
ACGTAA682090.3192394006261415No Hit
GTCTCA679590.31806932262827414No Hit
GTACTT676380.31656694247901246No Hit
GAGTGA675880.3163329268794389No Hit
GCGGCT649160.3038271332382325No Hit
GTTACA646290.3024838836966808No Hit
GGTACC643420.30114063415512904No Hit
GGCTAC639720.2994089187182853No Hit
TCGTGG622520.29135878209295785No Hit
TGCATA616920.2887378073777349No Hit
GCGCAG613470.28712309974067796No Hit
GCCAAT610900.2859202595588703No Hit
TTCCAT605760.28351457919525497No Hit
GCTCAA595280.27860961222819497No Hit
TAGCTT594350.27817434321298834No Hit
CGACAA593450.27775311513375606No Hit
CTATGC586970.27472027296328383No Hit
TATGGT581920.27235671540759177No Hit
TTAGTA568700.2661693429548691No Hit
CCTCGG565170.2645171928218804No Hit
GGATCG565040.2644563487659913No Hit
CGAGAG564940.2644095456460766No Hit
GACACG559870.26203662746640155No Hit
CTGTAT555430.2599585689421891No Hit
AACATA555340.25991644613426584No Hit
CAAGGA554930.2597245533426156No Hit
TCCACG554790.2596590289747351No Hit
TAATGA553380.25899910498393786No Hit
TCAAGC550470.25763713419442025No Hit
CGTCAG548780.2568461614678619No Hit
GGATTC548480.25670575210811786No Hit
TCTCTG545280.2552080522708476No Hit
CATACG542350.25383672085734704No Hit
GTTGGC533640.24976016911277713No Hit
GTACGC532860.2493951047774425No Hit
CGAAGC524290.2453840774007532No Hit
AACTAG521740.24419059784292846No Hit
CTGGAG514390.24075056852919835No Hit
ACAGCC508860.23816235599791574No Hit
AGTTAG501090.23452575358054398No Hit
CACCTA501000.23448363077262077No Hit
CTATTA480650.22495919586998037No Hit
CTTAGC474970.22230077865882572No Hit
GTGGTG450350.21077785053582784No Hit
AATCGG443740.20768416430946654No Hit
GGACTA443190.20742674714993573No Hit
GACTTG443170.20741738652595276No Hit
CTTGTA434460.20334083478138285No Hit
AAGCAT424930.19888049745351244No Hit
CACATT397930.18624365507654486No Hit
GAACCG395220.18497529052685663No Hit
GCTGTC372270.17423397450643419No Hit
ACGAAC371640.1739391148509716No Hit
GCATAA360360.16865972292459402No Hit
CAACGG356500.16685312249588682No Hit
AAGAGG354020.1656924051220024No Hit
GCCATA352680.1650652433151455No Hit
ACTAAT349190.16343181443012264No Hit
CTGTTC331360.15508681814933256No Hit
CATGAG330870.15485748286175055No Hit
TACGGC317290.148501619177335No Hit
AGTAGG286370.1340300944997114No Hit
TGGCAT282180.13206904377528567No Hit
GTCTGC275040.12872730101337648No Hit
AAGGCG264930.12399550559000085No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)