FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22621856
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAC4606922.036490728258548No Hit
GATCCT4048261.7895348639828668No Hit
CAGATC3398871.5024717688946478No Hit
GAGCCA3267781.4445233848186463No Hit
GAGAAG3064231.3545440303395089No Hit
TTACGT2949711.3039204210299986No Hit
CGTACT2904421.2838999594020932No Hit
GGAGGC2904311.283851333860493No Hit
ACGCTA2804871.2398938442539815No Hit
GTACGC2763681.2216857891766264No Hit
AACTCT2712091.1988804101661685No Hit
TGACAC2670611.1805441604791402No Hit
GGATCG2659891.175805380425019No Hit
CGAGAG2533911.1201158737815324No Hit
ATTCAT2500711.1054398012258588No Hit
ACTTGA2492541.1018282496361043No Hit
AGTGCA2455911.085635944283263No Hit
ACAGTG2382311.0531010364489988No Hit
CAGGAC2369471.0474251095931297No Hit
TACTCG2302121.0176530166225086No Hit
CTCGTC2282511.0089844087063413No Hit
GGCAGG2185740.9662071936095782No Hit
ATCCAC2141230.9465315312766556No Hit
GACCAT2102410.9293711355955939No Hit
ATACCT2076940.9181121124632744No Hit
TTCTGT2063100.9119941352292226No Hit
TACAGT2049110.9058098504384432No Hit
GTAAGA1981040.8757194811955307No Hit
TGATAA1969690.8707022094031541No Hit
TATTGG1955810.8645665501539749No Hit
CTAGTT1917410.8475918156317501No Hit
TAGAGC1914270.8462037774442557No Hit
ATCAGC1912140.8452622101387259No Hit
GGATTC1896560.8383750652466357No Hit
CAGCCT1888800.8349447543119362No Hit
GTACTT1885400.8334417830261142No Hit
CCTGAC1878180.8302501792956335No Hit
TCCTCC1876560.8295340576829769No Hit
GTCTCA1810390.800283584158612No Hit
CGAGCC1797150.7944308371514698No Hit
ATTGAA1752770.7748126413677109No Hit
TCAAGC1724830.7624617538012796No Hit
CAAGGA1676550.7411195615426073No Hit
AGTCAA1629680.720400660317173No Hit
ACACGA1592930.7041553089189498No Hit
GAAGAT1586370.7012554584380698No Hit
CTATTA1572100.6949473995413993No Hit
TATTCT1568240.6932410850816131No Hit
CGTCAG1560520.6898284561620408No Hit
TTGAAT1534090.6781450646666657No Hit
TCGGAC1440200.6366409546590696No Hit
GGTACC1414700.6253686700154045No Hit
TTCTAC1398600.6182516589266593No Hit
ATGTGA1383820.6117181543371154No Hit
AATGTA1375600.6080845002284516No Hit
GATCAG1374490.6075938243086686No Hit
CCAGCG1371550.6062941961968107No Hit
GGCGTT1371470.6062588321665561No Hit
AGGTGT1338030.5914766675201186No Hit
ACTAAT1328960.5874672705899993No Hit
ATTCGA1327070.5866317953752336No Hit
ACCGCT1308380.5783698738069945No Hit
TGCGGA1298540.5740200980856743No Hit
TCTTCA1290630.5705234795942472No Hit
AGCCTA1275380.5637822113269574No Hit
GCCAAT1246400.5509715913672159No Hit
CAGTCC1218250.5385278732213661No Hit
TTCCAT1195630.528528693666868No Hit
GACACG1189550.5258410273675157No Hit
TAGCTT1179240.5212834879684497No Hit
AGGCTT1177790.5206425149200843No Hit
AAGTTG1165470.5151964542608706No Hit
CCGTCG1146010.5065941539014306No Hit
GCGGCT1143430.5054536639257186No Hit
CCGCTC1139580.5037517699697143No Hit
TCTCTG1129170.4991500255328299No Hit
GAGTCG1123170.49649772326373226No Hit
AGTTCC1113220.49209932200081197No Hit
TACGAA1103670.48787774088916486No Hit
TGTGAT1097200.4850176749423213No Hit
TTAGCG1086400.4802435308579455No Hit
TCTCGC1074620.4750361774029505No Hit
CAACGG1059950.4685512983550068No Hit
ACGTAA1049390.46388324636139494No Hit
ACCAGG1039180.4593699120001471No Hit
AGTAGG1015150.4487474414124111No Hit
CCGGAA1012250.4474654953156806No Hit
GATTCA983860.43491568507906686No Hit
TTCACA975460.43120246190233025No Hit
TAAGTC971520.42946078341228944No Hit
TCCACG960890.42476178789220476No Hit
AACCTT957660.4233339651706739No Hit
AATCGG924980.4088877588116554No Hit
TTAGGC922130.407627915233834No Hit
TGACCA920660.4069781011779051No Hit
AAGCAT919370.4064078561900491No Hit
GAGTGA915800.404829736339936No Hit
GGTAAT907030.4009529545232716No Hit
GAGATA898540.39719994681249854No Hit
GTTACA896440.39627164101831436No Hit
AACATA891920.3942735733089274No Hit
CGACAA888570.39279270454201465No Hit
CGAATT879710.3888761381913137No Hit
AGAACG874420.386537691690726No Hit
CACCTA868260.3838146613611191No Hit
GTCGAG863220.38158672745507705No Hit
TGCGAC850380.3759108005992081No Hit
TATAAG849730.3756234678533892No Hit
AGTTAG821410.3631046011432484No Hit
CTCCGC801120.3541353989699165No Hit
GGCCGT799710.3535121079366786No Hit
CTGAGG799160.353268980228678No Hit
ATTGCT792390.35027629916837943No Hit
AACAAT792090.3501436840549246No Hit
TAATGA783360.3462845842533875No Hit
CTGTAT780410.34498053563774783No Hit
GCATAA770250.34048930379540915No Hit
CTTGTA748010.3306581033846206No Hit
GTCTGC742220.32809863169494136No Hit
GACTTG734690.32476999234722387No Hit
GCCTGG727220.3214678760221973No Hit
TGGCAT721480.3189305068514272No Hit
GCGCAG712640.31502278150829005No Hit
TTCGTT709360.31357285626785003No Hit
CGTGAA702760.3106553237718426No Hit
GCGTAT699550.30923634205787537No Hit
AATTGC688600.3043958904167722No Hit
CACATT678200.2997985664836696No Hit
CCTCGG675360.29854314340963No Hit
TCATGT662620.2929114215915794No Hit
TATGGT657650.2907144312120102No Hit
CTTAGC649000.28689069544072776No Hit
AAGGCG626300.27685615185597506No Hit
GAATAC622420.2751409963886252No Hit
CTATGC614440.2716134343707254No Hit
GTTGGC600900.26562807225012836No Hit
CATGAG594820.26294040595077606No Hit
GGCTAC580750.2567207571297421No Hit
AACTAG579830.25631407078181384No Hit
ACCTGC561810.24834832296695727No Hit
CGTTGC552910.2444140746011291No Hit
AGAGGT552510.24423725444985595No Hit
GACCGA537220.23747830416743881No Hit
CCGACT524740.23196151544771568No Hit
AGGACT522100.2307945024493127No Hit
CGTCTC520750.23019773443876573No Hit
GACTAA518910.22938436174290916No Hit
GCCATA515540.22789465196843264No Hit
TGCATA509490.22522024718042588No Hit
TTAGTA508050.22458369463584246No Hit
CATTAA508030.22457485362827878No Hit
CAGTAG497770.22003941674812183No Hit
CAAGCT494360.21853202495851798No Hit
CATAAC482320.21320973840519541No Hit
AAGAGG479910.21214439699377452No Hit
CCATGA464700.205420810741612No Hit
ATCCGG453360.20040795945301748No Hit
GCTGTC443160.1958990455955515No Hit
ACAGCC421890.18649663405160036No Hit
CGAAGC419320.1853605645796702No Hit
GTGGTG414290.18313705117741003No Hit
TCAATG413210.18265963676897246No Hit
AGATGC412140.1821866428643167No Hit
AGACGG412110.18217338135297123No Hit
GCTTCT411950.18210265329246195No Hit
AAGCGC406910.17987471938641994No Hit
TCCAGA398790.17628527031557448No Hit
ACGAAC389520.1721874633098186No Hit
GGACTA382220.16896049554908316No Hit
ACTAGC380650.16826647645533593No Hit
GCTCAA379880.16792609766413508No Hit
CGCTTG373470.16509255473998244No Hit
GAACCG371400.16417751045714374No Hit
TGTGTG354670.15678200763014316No Hit
GGCCAG341320.15088063508140093No Hit
CTGGAG335950.14850682455055855No Hit
GGTGGT328460.14519586721796832No Hit
GCACAT316720.1400061957781006No Hit
ATGGCC290200.12828301974868905No Hit
TACGGC284480.12575449158548263No Hit
ATGCCG280650.12406143863704197No Hit
CCACTT280570.12402607460678734No Hit
CCTGCT257550.11385007490101608No Hit
CATACG254610.11255044678915822No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)