FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24193882
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACT4199031.7355751342426156No Hit
TCATGT3309941.3680896682888675No Hit
GGCCGT3227591.3340521376437233No Hit
CGTCTC3107061.2842337579393004No Hit
TGTGAT3053241.2619884646870643No Hit
ATTCGA2936061.2135547325559413No Hit
CTGTAT2928751.210533307552711No Hit
AGAGGT2841771.1745820699629765No Hit
TCTTCA2780671.1493277515365248No Hit
CCACTT2727321.1272767222721845No Hit
GATCAG2702241.1169104652159583No Hit
GACTAA2681371.1082843174981178No Hit
TTCACA2661591.1001086969011422No Hit
GACCAT2634261.0888124526688194No Hit
TCCTCC2571691.062950542620651No Hit
CGAGCC2548871.0535184060168599No Hit
TGATAA2543151.0511541719514048No Hit
ATGTGA2539421.0496124598772532No Hit
TTCTAC2489501.0289791443969183No Hit
ACAGTG2441961.0093295486850766No Hit
CCGGAA2348460.9706834149228304No Hit
AGAACG2307970.9539477790294256No Hit
GAAGAT2263370.9355133665610175No Hit
GCTCAA2255770.9323720765439792No Hit
GGCTAC2241700.9265565567361203No Hit
GTGGTG2236500.9244072530402522No Hit
GCCAAT2233560.923192069796819No Hit
GCGTAT2232890.922915140282159No Hit
TTACGT2228680.9211750309437734No Hit
CAAGCT2150660.8889272089530733No Hit
TAGCTT2146550.8872284323780698No Hit
ATTGAA2134780.8823635661279989No Hit
GTCGAG2097310.8668761796887329No Hit
GGTACC2091100.8643094150827056No Hit
AGGTCA2058180.8507026693773244No Hit
GATCCT2049910.8472844498456262No Hit
TCTCGC2043170.8444986215936741No Hit
TATAAG2011000.8312018716136583No Hit
ACCAGG2000810.8269900630250243No Hit
GGTAAT1997640.8256798144258122No Hit
CCAGCG1992710.8236421091910756No Hit
AACCTT1983250.8197320297751307No Hit
ATACCT1923320.7949613046802494No Hit
CTCCGC1921830.7943454465058564No Hit
ACTTGA1917020.7923573405871782No Hit
TGCGAC1904130.787029547387228No Hit
TACAGT1897480.7842809186223194No Hit
AATTGC1850120.7647057218845656No Hit
TAAGTC1835640.7587207377468403No Hit
CAAGGA1809700.7479990189255283No Hit
CATACG1804830.745986113348821No Hit
TACTCG1774530.7334622860440504No Hit
ACACGA1764100.7291512788232992No Hit
CTAGTT1750030.7233357590154403No Hit
GAGAAG1740500.719396746665128No Hit
TATTGG1730920.7154370679331246No Hit
TCCAGA1726320.7135357608175489No Hit
CTGAGG1720230.7110185955275801No Hit
CAGATC1665230.6882855756674353No Hit
ATTCAT1650420.682164193410549No Hit
AGTGCA1623160.6708968821125936No Hit
CAGGAC1596490.6598734341185925No Hit
CCTGAC1575330.651127421386944No Hit
CGTGAA1556080.6431708644358933No Hit
CAGTCC1512290.6250712473508798No Hit
CCGCTC1509080.623744465646315No Hit
TAGAGC1461120.6039212723282688No Hit
CTATTA1459630.6033054141538757No Hit
CAGCCT1439500.5949851288850627No Hit
TTGAAT1431650.5917405069595694No Hit
CGAATT1421050.5873592340410687No Hit
CTTCAC1381790.5711319911372635No Hit
CCGTCG1373380.5676559057368304No Hit
GACACG1371470.5668664499562328No Hit
GTACGC1365980.5645972812465564No Hit
TCCACG1332020.5505606748020017No Hit
AACATA1313920.5430794446298449No Hit
CCTGCT1304130.539032967094739No Hit
CTGTTC1281540.5296958958467269No Hit
TCAAGC1264560.5226775926244495No Hit
AAGCGC1256890.5195073696730438No Hit
TCTCTG1256450.5193255055141627No Hit
CTTGTA1255510.5189369775383711No Hit
GCGGCT1239330.5122493364231503No Hit
TTAGGC1217600.5032677269402239No Hit
AAGCAT1211700.5008290939006812No Hit
ATCCGG1206440.4986549905467837No Hit
GTACTT1200810.4963279559683725No Hit
AGGTGT1167470.48254761265678653No Hit
GAGCCA1159590.4792905909022785No Hit
AACTCT1144650.47311547605299553No Hit
TTAGCG1144110.47289227913073234No Hit
ACCTGC1142520.47223508819295723No Hit
GCGCAG1056320.43660624615760296No Hit
ACGAAC1037700.4289100856158594No Hit
CTATGC1018360.42091632917776484No Hit
ACTAAT995550.41148832585031203No Hit
GTCTGC988910.4087438303617419No Hit
CCATGA949440.3924297886548343No Hit
TCGTGG946130.3910616741868874No Hit
GTTGGC944650.3904499492888326No Hit
CTTAGC903780.3735572488945759No Hit
TGACCA901550.3726355282711555No Hit
GCTGTC896060.37036635956147923No Hit
CGCTTG895370.37008116349414283No Hit
GTCTCA892640.368952779053812No Hit
AATCGG875240.3617608782253299No Hit
GCACAT872350.3605663613635877No Hit
TTCTGT864210.3572018744242863No Hit
TCGGAC840830.3475382743455556No Hit
ATCCAC832980.3442936524200622No Hit
CCTTAT832270.3440001898000494No Hit
AGGCTT812360.335770836610677No Hit
GATTCA807310.3336835320598819No Hit
AAGGCG798290.3299553168028182No Hit
AGTTAG790350.3266734953902809No Hit
AACTAG768560.3176670862493253No Hit
TGACAC767080.31705536135127055No Hit
ACGCTA753970.31163663607187964No Hit
GGATCG752760.3111365096349565No Hit
ATCAGC731170.3022127660207651No Hit
GGCAGG722420.29859614922483296No Hit
GACTTG708830.2929790266812081No Hit
GCATAA690360.2853448652845376No Hit
CGAGAG686960.2839395513295469No Hit
CTACCG671950.27773550354589643No Hit
CATGAG651870.2694358846587745No Hit
TGCGGA634860.2624051816074824No Hit
CGTCAG633130.26169012480097237No Hit
TGCATA623960.25789991039883553No Hit
AGATGC613680.25365090232315757No Hit
CTCGTC611250.25264651617297296No Hit
CACCTA605960.2504600129900609No Hit
AGCCTA597740.24706245984005376No Hit
GAGATA596740.246649132206233No Hit
GTAAGA595480.24612833938761872No Hit
AAGAGG592460.24488008993347987No Hit
ATGCCG583920.24135027194065012No Hit
AGTCAA579630.23957709639155883No Hit
TTCCAT577190.23856857696503603No Hit
ACGTAA571960.23640687344015318No Hit
TTAGTA553110.22861564754263083No Hit
GCCTGG551180.22781792520935665No Hit
AATGTA520170.21500063528457317No Hit
GAGTCG516130.21333079164393712No Hit
CGAAGC515150.21292573056279268No Hit
AACAAT511150.21127242002750946No Hit
TGGCAT509830.21072682755086594No Hit
ATTGCT506620.20940004584630115No Hit
CCTCGG489880.20248094125614072No Hit
GCCATA476520.19695888406829465No Hit
GGAGGC476500.19695061751561818No Hit
CATTAA468850.19378866111688897No Hit
AGTTCC465780.19251974528105906No Hit
CGACAA463510.19158149155228582No Hit
CATAAC456580.18871713104990756No Hit
CACATT438230.18113256896929564No Hit
GGCGTT421090.17404813332560687No Hit
CAGTAG417920.17273788472639487No Hit
GAATAC396490.16388027353361484No Hit
ACAGCC395950.1636570766113516No Hit
TAATGA365910.1512407144913743No Hit
ACCGCT350300.14478867012743138No Hit
TGTGTG340890.14089925709317752No Hit
ACTAGC298910.12354776302537973No Hit
CCGACT289560.1196831496491551No Hit
GGACTA286500.11841836708966341No Hit
AGACGG285920.11817863706204733No Hit
TCAATG284850.11773637649385907No Hit
TATTCT284640.1176495776907567No Hit
GGATTC278090.11494228168923036No Hit
GACCGA274050.11327243804859427No Hit
GTTACA270180.11167286010570772No Hit
TTCGTT242360.10017408533281266No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)