FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26960908
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTAG9668843.5862442021611436No Hit
TACGGC9248613.430377789946837No Hit
TATGGT8543333.168784226406618No Hit
CCTCGG8338373.0927630478914137No Hit
GTTACA7368512.7330348072846804No Hit
AGTAGG7107422.636194597006896No Hit
ACAGCC7023432.6050420853778364No Hit
CAACGG6693432.482642646901952No Hit
ACGTAA6277952.3285380447869186No Hit
GAGTCG6066932.25026916749243No Hit
GAGTGA4310561.5988185561109438No Hit
CAGCCT3436471.27461211617947No Hit
GAGAAG3012401.117321419590171No Hit
GGAGGC2996871.1115612278340181No Hit
CAGGAC2794261.0364116816837177No Hit
GTACGC2675580.9923923927191176No Hit
CGTGAA2632870.9765509381212236No Hit
TTGAAT2569300.9529723553820961No Hit
TAAGTC2552730.9468264199410494No Hit
AAGGCG2546160.9443895583932114No Hit
TTACGT2422990.8987048952505605No Hit
CTATGC2335850.8663840253451405No Hit
CTCGTC2302660.854073609093581No Hit
TCAAGC2295350.8513622760776454No Hit
AACCTT2285470.8476977110711553No Hit
GGTACC2273270.8431726409214407No Hit
TAATGA2212680.8206993622024896No Hit
CATACG2208140.819015442654973No Hit
CCTGCT2202760.8170199609004267No Hit
CGTCAG2182130.8093681414587371No Hit
CGAGCC2173370.8061189927282865No Hit
TTAGTA1983180.7355761163533513No Hit
ATCCGG1969790.7306096664103449No Hit
CAAGCT1961070.7273753539754669No Hit
GAAGAT1957730.7261365232951353No Hit
GCACAT1872360.6944721594688131No Hit
TCTCTG1833160.6799325898074353No Hit
CCATGA1822230.6758785720421583No Hit
CACCTA1807750.6705078330447921No Hit
TCCACG1786270.66254074232218No Hit
TCGTGG1769510.6563243344771622No Hit
GAGCCA1764800.654577360673461No Hit
ACGCTA1738710.6449003868860796No Hit
ACACGA1674920.6212402045213017No Hit
TGCATA1647870.6112071596401724No Hit
CTGTTC1635610.6066598350470986No Hit
AGGCTT1555650.5770020801970023No Hit
AAGCAT1510070.5600961213917572No Hit
GATCCT1476790.5477523234751589No Hit
ACCTGC1436420.5327787921682756No Hit
TGGCAT1380350.5119820148490548No Hit
CATAAC1285160.47667534045960175No Hit
ACTTGA1257830.4665384415094625No Hit
AAGAGG1250820.4639383807103233No Hit
GACCGA1240680.4601773797826097No Hit
ACTAGC1239250.45964698221588085No Hit
AGCCTA1228020.4554816922338075No Hit
AGATGC1223810.4539201721247667No Hit
ACAGTG1202610.4460569354711644No Hit
GATCAG1184450.43932125728109755No Hit
CGTTGC1177790.4368510140682205No Hit
ACGAAC1173700.43533400284589824No Hit
AGGACT1096040.4065293349912399No Hit
GAGCAC1080600.40080252490012575No Hit
CATTAA1073140.3980355557757921No Hit
TATTGG1072780.3979020291156366No Hit
AACAAT1064300.3947567344541957No Hit
TGATAA1058800.3927167438129309No Hit
CAGATC1054210.39101427889594814No Hit
CCTTAT1040180.3858104482237764No Hit
GCCAAT1020240.3784145548807184No Hit
TCAATG999210.37061437248330065No Hit
GGCGTT974280.3613676512675315No Hit
CAGTAG971380.36029201983850095No Hit
GCTTCT970470.359954494114219No Hit
GAATAC956620.354817426772125No Hit
AATGTA930540.3451441620586369No Hit
GGTGGT920220.34131639780084555No Hit
TTCGTT895860.33228109379698934No Hit
TGACCA881300.32688068220847755No Hit
CTGTAT875420.3246997467592709No Hit
ATTCAT866810.32150623413721824No Hit
ATGGCC861980.31971475144679845No Hit
NNNNNN852610.316239349208862No Hit
ATCAGC844840.31335739879383884No Hit
CAAGGA838360.31095391891103963No Hit
TGTGTG829790.30777524258455985No Hit
TCTTCA829640.3077196064761617No Hit
AGTTCC817370.30316857280919474No Hit
GGCAGG815260.3023859582177277No Hit
ATACCT814410.30207068693680494No Hit
GGATCG810430.30059447552730795No Hit
TACAGT809910.3004016036848611No Hit
CTCCGC807360.29945578984209287No Hit
CTTCAC800250.29681863830402155No Hit
CTAGTT794920.2948417019189413No Hit
GTCTCA794400.29464883007649445No Hit
TAGAGC793430.29428904990885324No Hit
GGCCAG791510.29357690772135714No Hit
GTCTGC789900.2929797468245506No Hit
CCGACT787920.2922453501936953No Hit
GAGATA778430.28872543906904025No Hit
GTACTT773780.2870007197086982No Hit
TTCTGT759130.2815669264551476No Hit
AAGTTG757520.28096976555834097No Hit
ACTAAT753970.27965304432625193No Hit
ACCGCT750490.2783622866114153No Hit
GGCTAC747020.2770752379704719No Hit
GCTCAA745210.27640389559580114No Hit
CGAATT743910.275921715989684No Hit
CCTGAC743170.27564724452158657No Hit
TACTCG740710.27473481234385727No Hit
ATGCCG732280.2716080630518824No Hit
AACTCT731460.2713039189926393No Hit
TGACAC727310.26976465332695765No Hit
TTAGGC722470.26796946156264473No Hit
TAGCTT721450.2675911360255374No Hit
CCGGAA716590.265788526113438No Hit
AGAACG713930.2648019124578445No Hit
TGCGAC713350.26458678617203846No Hit
GACCAT708920.262943666437347No Hit
GTAAGA692030.2566790406317176No Hit
ATTGAA691580.2565121323065232No Hit
TTCACA688820.2554884279119976No Hit
AGGTCA683670.2535782548569952No Hit
CGTACT674690.2502475065008938No Hit
TCCAGA670940.2488566037909406No Hit
GGCCGT652010.2418353269110966No Hit
CCAGCG645000.23923526611195736No Hit
TATAAG617500.22903531290563361No Hit
GCGCAG611840.22693597708207752No Hit
CTTAGC594960.2206750603503413No Hit
AGACGG594680.22057120628133148No Hit
TATTCT587530.2179192184476873No Hit
ATGTGA576530.2138392371651578No Hit
ATCCAC573660.2127747329578069No Hit
CTTGTA571500.21197357299687383No Hit
AGTGCA558660.20721112211799395No Hit
TACGAA554540.20568298367399201No Hit
TTAGCG554180.20554945701383645No Hit
GGTAAT551130.20441818947640783No Hit
GGATTC548700.20351688452035815No Hit
CAGTCC546230.20260074326873562No Hit
GGACTA534110.19810534571016672No Hit
GCCATA532060.19734498556205896No Hit
ATTCGA521550.19344674890029667No Hit
AGAGGT508360.1885544804351545No Hit
TTCCAT507470.1882243728586589No Hit
TCTCGC497070.18436693600972193No Hit
CCGCTC494530.18342483124084694No Hit
AGGTGT489260.18147014929912597No Hit
TGTGAT488160.18106215117087301No Hit
AATTGC487400.18078026155498916No Hit
CTACCG483390.1792929229238125No Hit
ATTGCT482850.17909263293357924No Hit
AAGCGC482750.17905554219464714No Hit
AGTCAA474880.17613650104069195No Hit
GACTTG473240.17552821292220572No Hit
ACCAGG469380.1740965103994272No Hit
GCGTAT461170.1710513607331029No Hit
CCACTT444390.16482753474029882No Hit
GTCGAG440140.16325117833568514No Hit
TCGGAC435720.1616117676748869No Hit
CTGGAG435670.16159322230542086No Hit
AACATA418440.15520248798742237No Hit
TGCGGA416200.15437165543534365No Hit
CCGTCG414850.15387093045976047No Hit
TCCTCC412880.15314024290279837No Hit
AGTTAG400490.14854470034911288No Hit
AATCGG392820.14569984067302186No Hit
CGACAA386110.14321105209067886No Hit
GCGGCT384190.14249890990318279No Hit
GATTCA383850.1423728013908137No Hit
TTCTAC377160.1398914309562571No Hit
CTGAGG374230.138804672305547No Hit
CGTCTC372610.13820380233484716No Hit
CATGAG372200.1380517303052256No Hit
GACACG354330.1314236152580618No Hit
CGAAGC347280.12880871816334968No Hit
GTGGTG345640.12820043004486348No Hit
GCCTGG343520.12741410637950326No Hit
GAACCG333880.12383855914645012No Hit
GCTGTC317880.1179040409173163No Hit
CGCTTG301270.11174326918069674No Hit
CACATT299860.11122028976175431No Hit
GACTAA297690.11041542072692805No Hit
GTTGGC284540.10553798855735869No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)