FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21559341
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAT4617982.1419856942751636No Hit
CTTCAC4107001.9049747392557128No Hit
CGTACT3481291.6147478719317072No Hit
CCGACT3326951.5431594128967114No Hit
AGCCTA3295131.5284001491511265No Hit
CTGTAT2831211.3132173195831913No Hit
GAGAAG2703091.2537906423021No Hit
GTACTT2617181.213942485533301No Hit
GGCGTT2563101.188858230870786No Hit
TGACAC2560941.187856344959709No Hit
AGTGCA2544341.180156666198656No Hit
CTAGTT2474231.1476371193349555No Hit
GTACGC2470771.1460322465329529No Hit
ATACCT2460411.1412269048483439No Hit
TCGGAC2399841.1131323540918991No Hit
AGTTCC2301391.0674676930060154No Hit
GCACAT2280871.057949776850786No Hit
TTACGT2279341.0572401076637732No Hit
CCGTCG2125810.9860273558454316No Hit
CCTGAC2099590.9738655740915272No Hit
CTCGTC2075530.9627056782486997No Hit
GAATAC2046100.9490549827102787No Hit
TACGAA1953640.906168699683353No Hit
AGGTCA1923680.892272171027862No Hit
GGTGGT1921530.8912749234774848No Hit
TGACCA1918100.8896839657575805No Hit
AAGAGG1903360.8828470220866212No Hit
CCGGAA1874910.8696508858967442No Hit
GACCGA1852350.8591867441588312No Hit
TTCACA1842650.8546875342803845No Hit
AACTCT1782370.8267274959842233No Hit
CAGCCT1763930.8181743588544751No Hit
TAGAGC1746480.8100804194339707No Hit
TCTTCA1733200.8039206764251282No Hit
CAGTCC1713200.794643955026269No Hit
CTACCG1706560.7915640835218479No Hit
GAACCG1700690.7888413657912827No Hit
CAGATC1697700.7874544959421532No Hit
ACCTGC1666790.7731173230202166No Hit
TGCGAC1661800.7708027810312013No Hit
AACAAT1636950.7592764546931189No Hit
GGAGGC1622900.7527595579104204No Hit
CCATGA1614290.7487659293482115No Hit
TAGCTT1564800.7258106822467347No Hit
AGGCTT1560110.7236352910787023No Hit
AGGACT1543790.7160654864172332No Hit
TTAGGC1502610.6969647170569824No Hit
GACTTG1497620.694650175067967No Hit
CAGTAG1465960.6799651250935732No Hit
CATTAA1465150.6795894178769193No Hit
AGTTAG1450430.672761750927359No Hit
TTCTGT1444780.6701410771321813No Hit
GTCTCA1439540.6677105761256802No Hit
AAGTTG1412290.6550710432197347No Hit
TTCCAT1412280.6550664048590353No Hit
CCTTAT1376200.6383311994554935No Hit
TTCGTT1369460.635204944344078No Hit
ACAGTG1339780.6214382897881712No Hit
TGATAA1316130.6104685667340204No Hit
CCAGCG1310920.6080519808096175No Hit
CGACAA1308330.6068506453884652No Hit
AGAGGT1274740.5912703917990815No Hit
TTGAAT1266500.5874483825827514No Hit
CGTTGC1240510.5753932831249341No Hit
GACCAT1236690.573621429337752No Hit
AAGGCG1233880.5723180499812124No Hit
GAGTCG1177170.5460139064547475No Hit
ACTTGA1174670.54485431627989No Hit
TACTCG1172520.5438570687295127No Hit
TATGGT1169980.5426789251118576No Hit
GGTAAT1155080.5357677676697075No Hit
GAGCAC1148760.5328363237076681No Hit
GCTTCT1118700.5188934114451829No Hit
TACGGC1110170.5149368897685694No Hit
ATGCCG1084840.5031879221169144No Hit
GCATAA1081130.5014670902974261No Hit
GTCGAG1079880.5008872952099974No Hit
CTGAGG1075220.4987258191240632No Hit
TCAATG1053730.48875798198098913No Hit
AGATGC1048180.4861836917928057No Hit
AACTAG1040930.4828208802857193No Hit
TGTGTG1037550.48125311436931206No Hit
GTCTGC1004140.465756351272518No Hit
TACAGT1002280.4648936161824242No Hit
ACGCTA979510.454332068869823No Hit
GGCAGG978150.4537012518147006No Hit
AATTGC977050.4531910321377634No Hit
CAAGGA975470.4524581711472535No Hit
CCTGCT970510.4501575442403365No Hit
ATGGCC960330.4454356930483172No Hit
ACTAGC957360.4440580999205866No Hit
TCTCGC954520.44274080548194866No Hit
GCTCAA949010.44018506873656293No Hit
AAGCGC942390.43711447395354064No Hit
ACTAAT937940.43505040344229445No Hit
TTAGTA931500.43206329915186187No Hit
ACAGCC927750.43032391388957575No Hit
TTCTAC923150.4281902679678382No Hit
GGCCAG922470.427874859440277No Hit
GATTCA917000.425337676137689No Hit
AATGTA916400.4250593744957233No Hit
GTAAGA915740.42475324268956083No Hit
CCTCGG905670.42008241346523534No Hit
GATCAG905260.4198922406765587No Hit
TCTCTG895740.4154765212907018No Hit
ATGTGA894980.4151240058775451No Hit
TGCGGA891220.41337998225455963No Hit
AGAACG886460.41117212256163116No Hit
GACTAA883880.4099754255011783No Hit
ACGTAA880380.408351999256378No Hit
CGAATT871040.4040197703631108No Hit
CAACGG852990.3956475293006405No Hit
TTAGCG845080.3919785859873917No Hit
CGTGAA818830.3798028891513892No Hit
GTTACA818570.379682291773204No Hit
AGACGG814890.37797537503581397No Hit
CGTCAG808350.374941887138387No Hit
TCATGT800820.37144920153171657No Hit
CACCTA783780.36354543489988866No Hit
ATCAGC780760.3621446499686609No Hit
CCACTT771520.357858804682388No Hit
GGCCGT755430.3503956823170059No Hit
GCCAAT743250.34474615898510075No Hit
TATAAG737640.3421440386327207No Hit
TCGTGG735260.34104010878625646No Hit
TCCTCC726190.3368331156318739No Hit
ATTGAA723130.3354137772578485No Hit
GCGTAT690000.3200468882606384No Hit
ATTGCT684060.31729170200517726No Hit
CATGAG671510.3114705593273932No Hit
AGTCAA658750.3055520110749211No Hit
ATTCGA646620.29992567954651306No Hit
CGCTTG618390.28683158729202346No Hit
AGTAGG605640.2809176774002508No Hit
GAGTGA598800.27774503868184097No Hit
CTCCGC575340.26686344448097926No Hit
CTTGTA573300.26591721889829567No Hit
CGTCTC565800.2624384483737235No Hit
GCGCAG552410.2562276833971873No Hit
ACCAGG552360.2562044915936902No Hit
ATCCAC542020.25140842663048No Hit
AACCTT539360.25017462268443175No Hit
GGCTAC529180.24545277149241249No Hit
GAGATA527250.24455756787742258No Hit
GCCTGG503520.23355073793767628No Hit
AGGTGT486100.22547071359927004No Hit
GGATTC473190.2194825899363065No Hit
GTTGGC457510.212209640359601No Hit
AATCGG449540.20851286688215562No Hit
TAAGTC444920.20636994423901917No Hit
TCAAGC442330.20516860881786692No Hit
ACCGCT434960.20175013698238733No Hit
GCCATA429940.19942167991127374No Hit
GCGGCT419840.1947369356048499No Hit
TGTGAT407510.18901783686245324No Hit
AACATA404170.18746862438884382No Hit
GGACTA368470.1709096766918803No Hit
CACATT347210.16104852184489313No Hit
TATTCT339200.15733319492465006No Hit
CTGGAG301450.13982338328430355No Hit
ACACGA299170.1387658370448336No Hit
CAGGAC280720.1302080615543861No Hit
TCCAGA276600.12829705694622112No Hit
AAGCAT268550.12456317658318035No Hit
GACACG267190.12393235952805792No Hit
GAAGAT264990.12291192017418343No Hit
GATCCT261340.12121891851889165No Hit
GAGCCA254220.11791640570089781No Hit
CCGCTC252190.11697481847891363No Hit
CGAAGC249860.11589408043594654No Hit
TGCATA244250.11329196008356655No Hit
GGTACC231990.10760532986606594No Hit
CGAGCC221610.10279071146005808No Hit
TATTGG215620.10001233340109977No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)