FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26462368
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAG4223441.5960174085705405No Hit
TTACGT4060451.5344242813039257No Hit
GTACGC3483411.316363675389897No Hit
TTGAAT3276601.2382111835191771No Hit
AAGGCG3267461.2347572220294118No Hit
CAGCCT3265751.2341110213568187No Hit
GGAGGC3173321.1991821744750886No Hit
CGTGAA3126121.1813455243310047No Hit
CGTCAG3005071.1356013188237728No Hit
ACGCTA2947721.1139290331084506No Hit
GTCTCA2812191.0627129061163385No Hit
TCTCTG2696311.018922418432092No Hit
TAGAGC2684051.0142894241361922No Hit
TGATAA2466680.9321463596908636No Hit
TACAGT2454980.9277249866678599No Hit
TAGCTT2408350.9101037367479735No Hit
CCTGCT2359230.8915415279539609No Hit
CTCGTC2281700.8622433185117825No Hit
ACTAAT2274990.8597076421883333No Hit
AATGTA2271150.8582565248884755No Hit
GTACTT2216390.8375629875602969No Hit
CTTCAC2201430.8319096764129348No Hit
CCATGA2157620.8153540907601314No Hit
GGCTAC2155650.8146096373536941No Hit
CATTAA2083760.7874427564456816No Hit
CTGTAT2067000.7811092340640112No Hit
AAGAGG2011870.7602758755376692No Hit
GATCAG1974300.7460783554971346No Hit
AGTTCC1970080.7444836380478119No Hit
CGTTGC1963910.7421520250946552No Hit
AACTCT1963330.7419328459191559No Hit
TCGTGG1958700.7401831914664629No Hit
ACCTGC1944010.7346319120042469No Hit
AGTGCA1919670.7254339445358783No Hit
TCGGAC1871230.707128704430382No Hit
AGGACT1853700.7005042027984797No Hit
GCACAT1849350.6988603589822346No Hit
GACTTG1838100.6946090387678079No Hit
TTAGTA1837070.6942198067837315No Hit
ATTGAA1832020.6923114363763666No Hit
AGTTAG1828500.6909812455181638No Hit
AGGCTT1822920.6888725906918081No Hit
TACGAA1818310.6871304941417186No Hit
GACCGA1814820.6858116401374208No Hit
CACCTA1794920.6782915270470126No Hit
ACTTGA1788550.6758843350678216No Hit
TTCGTT1782920.6737567854849574No Hit
ATTCAT1780350.6727855950004172No Hit
ACTAGC1759070.6647439866303726No Hit
GGCAGG1748960.6609234668643411No Hit
GAGCAC1745170.6594912443209919No Hit
ACAGTG1739040.6571747471730421No Hit
CGAAGC1706450.6448591448807605No Hit
TTCTGT1687690.6377698322387475No Hit
AGCCTA1683150.6360541883477699No Hit
GCTTCT1682310.6357367564384261No Hit
CTAGTT1668300.6304424456647266No Hit
GCCAAT1653320.624781576614761No Hit
AGATGC1641640.6203677614943606No Hit
GGCGTT1608210.6077347272927351No Hit
CGACAA1608170.6077196114875283No Hit
CAAGGA1605250.6066161577074282No Hit
CAGATC1601180.6050781245276311No Hit
AAGTTG1600100.6046699977870461No Hit
GACCAT1571190.5937450495737948No Hit
CCGTCG1566050.5918026686047144No Hit
ATACCT1548600.5852083985832258No Hit
GCCATA1519240.5741133975613975No Hit
TTCCAT1516560.5731006386125383No Hit
GTGGTG1511230.5710864575687256No Hit
CAGTCC1511120.5710448891044066No Hit
TGACAC1507130.5695370875350233No Hit
GAATAC1488830.5626216066528891No Hit
CCTTAT1454440.5496257931262992No Hit
CAGTAG1453090.549115634700568No Hit
GGTGGT1444100.5457183574803283No Hit
CTTGTA1422670.5376200648407581No Hit
ATCAGC1411640.5334518815549689No Hit
GTCTGC1376840.5203011310250089No Hit
TCAATG1354590.511892964378698No Hit
TATAAG1301720.49191364884654315No Hit
GGCCGT1287230.4864379484103614No Hit
ATGCCG1248220.47169625938238025No Hit
GAACCG1235660.4669498965474292No Hit
AACAAT1233550.4661525378227678No Hit
TACTCG1231540.46539296861112356No Hit
CCGACT1229900.4647732205976427No Hit
TTAGGC1227080.46370755633055966No Hit
ATGGCC1201510.45404477785208036No Hit
CCTGAC1168500.44157045960512675No Hit
TGACCA1164720.44014201601307934No Hit
CCACTT1152420.4354939059119728No Hit
CTTAGC1151460.43513112658700837No Hit
AAGCGC1138520.4302411636025922No Hit
TGTGTG1125960.42549480076764107No Hit
GGTAAT1070540.40455185265354937No Hit
TTCACA1024710.38723291883780014No Hit
TCTCGC998830.3774529928689677No Hit
GAGTCG990660.3743655896554685No Hit
CTCCGC988860.3736853784211602No Hit
CAACGG982420.3712517337828572No Hit
TATGGT960430.36294181987039104No Hit
TTAGCG959920.3627490933540037No Hit
GTCGAG957320.3617665660155584No Hit
CGTACT949490.35880764714631735No Hit
TCCAGA919600.34751236170549815No Hit
AGTAGG909310.3436238208160358No Hit
AGACGG902570.3410768076386815No Hit
TGTGAT895990.3385902576821545No Hit
TGCGAC856690.3237389790664237No Hit
TACGGC845400.31947254304679007No Hit
ACAGCC834870.3154933073260866No Hit
ACGTAA834330.3152892439557941No Hit
GCGCAG827020.3125268305542422No Hit
TTCTAC823370.31114751332911705No Hit
TCTTCA814980.3079769731869801No Hit
GCTCAA793310.2997879857161687No Hit
TGCGGA787480.29758485710727023No Hit
ATTGCT780470.2949358122447696No Hit
CCTCGG778730.2942782747182716No Hit
AACTAG774240.2925815255838026No Hit
AGAGGT773780.29240769382392384No Hit
AGGTGT765560.289301395853916No Hit
TCATGT762290.28806567877825595No Hit
GAGATA751150.28385592702814805No Hit
AATTGC733800.2772994465196766No Hit
GGCCAG728750.2753910761123116No Hit
GTTACA724430.27375856914997176No Hit
AGGTCA713660.2696886385980272No Hit
GCGTAT713630.2696773017441221No Hit
TATTCT705100.2664538562837611No Hit
AGAACG700080.26455682273030134No Hit
CGAATT692460.26167726183839635No Hit
ATGTGA686590.259459017424291No Hit
TCCTCC673100.25436121211828056No Hit
CCAGCG666320.2517990831357194No Hit
ATTCGA664230.25100928231366143No Hit
GAAGAT653750.24704894134946653No Hit
CCGGAA649790.24555247663398833No Hit
AACATA639960.24183776750440472No Hit
CTACCG635150.24002009192828094No Hit
GAGTGA633530.2394079018174035No Hit
GTAAGA631570.2386672273622678No Hit
GTTGGC627960.23730302594234953No Hit
GCTGTC621320.23479380227801233No Hit
CATGAG612120.23131716708043662No Hit
CTGAGG610080.23054626101488726No Hit
CGCTTG608510.22995296566051837No Hit
GATTCA605170.22869079592574634No Hit
ATCCAC603770.22816174274350653No Hit
GACACG602210.2275722263404394No Hit
GGTACC601870.22744374199618111No Hit
GGACTA590850.22327933766169375No Hit
CATACG583670.22056605062706408No Hit
CGTCTC582450.22010501856825512No Hit
ACACGA570140.21545312951584678No Hit
GCATAA562420.21253577911092464No Hit
ACCAGG561680.21225613671459787No Hit
GGATTC558150.21092216690509327No Hit
CTGGAG552710.2088664173969616No Hit
CCGCTC542300.20493252909187873No Hit
CGAGCC527020.1991582915028617No Hit
AGTCAA525410.19854988034328597No Hit
CACATT525130.198444069706838No Hit
TATTGG513070.19388665443697253No Hit
GAGCCA511510.19329713803390536No Hit
ACCGCT508960.19233350545196862No Hit
GATCCT497650.18805951152973158No Hit
TCAAGC496840.18775341647429283No Hit
AATCGG496490.1876211531787329No Hit
GCCTGG491460.18572034067397145No Hit
CAGGAC487930.18438637086446685No Hit
TAAGTC483490.18270851648650643No Hit
GGATCG474830.17943594465922322No Hit
AACCTT465950.17608023590330238No Hit
TCCACG454230.17165130497769512No Hit
CGAGAG449620.16990920842760557No Hit
GCGGCT438570.16573346723921306No Hit
GACTAA436550.1649701190762671No Hit
NNNNNN426910.1613272100214161No Hit
AAGCAT408130.15423033947679965No Hit
TAATGA395290.14937816600540058No Hit
CTATTA394290.1490002708752293No Hit
ACGAAC367980.13905785000042326No Hit
CTGTTC360090.13607625742337193No Hit
TGGCAT352870.13334785458353537No Hit
CTATGC326570.12340921266003103No Hit
TGCATA325770.12310689655589402No Hit
ATCCGG308940.11674692151511157No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)