FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22796812
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAATT4738402.07853624445383No Hit
AGTGCA3868651.6970135999717855No Hit
TCCTCC3031391.3297429482683807No Hit
TCTCGC2941311.290228651269309No Hit
GAGATA2911571.277182967513177No Hit
ATCCAC2822571.2381424209665808No Hit
TTCCAT2813241.2340497434465838No Hit
GTAAGA2796211.2265794006635664No Hit
CACATT2754091.20810313301702No Hit
TGATAA2672021.172102485207142No Hit
AGTCAA2535061.1120239093080206No Hit
CTCCGC2533801.1114712004467993No Hit
ATCAGC2304141.0107290440435268No Hit
AGGTGT2160970.9479264030426711No Hit
TACTCG2115640.9280420437734891No Hit
GGATTC2093920.9185143957848141No Hit
CAGTCC2082430.9134742173598659No Hit
GGTAAT2014380.8836235522756428No Hit
ACTTGA1967320.862980314966847No Hit
ACAGTG1940760.8513295630985596No Hit
GACCAT1927410.8454734811165701No Hit
AGAACG1838950.8066698097962118No Hit
AGGTCA1811310.7945453074754487No Hit
TTCACA1792010.7860792114265802No Hit
ACCAGG1790270.785315946808703No Hit
TCAATG1769690.7762883687420855No Hit
TACGAA1763260.7734677989185506No Hit
GCCAAT1753450.7691645656418977No Hit
GCGTAT1713440.751613866008984No Hit
CTTCAC1655740.7263033094276515No Hit
TCTTCA1645020.7216008975290054No Hit
AGAGGT1640080.7194339278667561No Hit
CAGATC1628630.7144112957548626No Hit
CGACAA1620730.7109458989265692No Hit
TTAGTA1602480.7029403935953852No Hit
AATCGG1548070.6790730212627976No Hit
GGCCGT1519100.6663651040329674No Hit
ACCGCT1514220.6642244538403002No Hit
AATTGC1510140.6624347299087259No Hit
TCGTGG1492220.6545739816602426No Hit
AAGCGC1491340.6541879627730404No Hit
GAACCG1491160.6541090043642944No Hit
CCAGCG1483340.6506787001621104No Hit
TAGCTT1452910.6373303425057855No Hit
TCCAGA1449730.6359354106179408No Hit
CTTAGC1411430.6191348158681135No Hit
TCGGAC1400980.6145508415825862No Hit
ATTCGA1399690.6139849729865737No Hit
CAAGCT1379770.6052469090853582No Hit
GGCAGG1358290.5958245389750111No Hit
GATCAG1323500.5805636331957292No Hit
AACCTT1318780.5784931682552806No Hit
GCTTCT1283830.5631620772237803No Hit
CGTACT1283790.5631445309107256No Hit
ATTCAT1268900.5566129158761322No Hit
CATTAA1262320.5537265473786422No Hit
TTCTGT1261980.5535774037176777No Hit
GAGCAC1253520.5498663585066192No Hit
TTACGT1251250.5488706052407678No Hit
CTGTAT1248700.5477520277835339No Hit
GCCTGG1245720.5464448274609626No Hit
CTGGAG1237970.5430452293066241No Hit
CCTTAT1236360.5423389902061745No Hit
GCGGCT1201410.527007899174674No Hit
CAGCCT1197770.5254111846867009No Hit
GATCCT1183630.5192085630218821No Hit
CGAGCC1172490.5143219148361622No Hit
CCGTCG1166410.5116548752518554No Hit
GGATCG1163060.5101853715335285No Hit
GCACAT1163040.5101765983770011No Hit
CAGGAC1154550.5064523934311517No Hit
AAGCAT1153020.5057812469568114No Hit
GGTACC1149660.5043073566602208No Hit
TCAAGC1134280.49756079929070784No Hit
TGTGAT1128140.4948674402368191No Hit
TATTGG1122500.4923934100961134No Hit
CCATGA1120620.4915687333825449No Hit
ATACCT1116940.48995447258151703No Hit
TGACCA1110060.4869365067361174No Hit
GAGAAG1091780.47891784167014234No Hit
TTAGGC1090910.4785362093612037No Hit
CAGTAG1083000.47506642595464665No Hit
ACACGA1041270.4567612348603831No Hit
GGCGTT1040470.4564103085992901No Hit
TGCGAC1039590.45602428971208786No Hit
AGTTAG1032950.4531116017450159No Hit
ACTAGC1027040.4505191339911914No Hit
ACGCTA1027010.4505059742564004No Hit
AGACGG1023500.44896628528585486No Hit
GATTCA1023350.44890048661189996No Hit
TTCGTT1015190.44532103874875134No Hit
AACAAT1014800.44514996219646846No Hit
CCGGAA1011560.44372871083904186No Hit
AACATA1002780.43987729512354623No Hit
GGACTA993310.43572320550785787No Hit
GACTTG980170.4299592416694054No Hit
AACTAG977840.428937168933972No Hit
TATTCT972340.42652455088895763No Hit
TATGGT953910.4184400871490277No Hit
AACTCT948770.41618538592150517No Hit
TAATGA946670.415264204486136No Hit
CGAGAG943560.413899978646137No Hit
TGACAC936520.4108118275485186No Hit
GTTACA932700.4091361546517996No Hit
ATTGAA927750.4069647984112867No Hit
TGGCAT925720.4060743230237631No Hit
TACAGT924630.40559618599302394No Hit
ATCCGG921420.4041880943703883No Hit
GACTAA920410.40374504996575844No Hit
GCTGTC907990.3982969197622896No Hit
ACCTGC904020.3965554481916156No Hit
GCTCAA904000.3965466750350882No Hit
CCTGAC896050.3930593453154766No Hit
CTACCG895010.3926031411760557No Hit
GACACG894050.39218202966274407No Hit
AATGTA884810.3881288313471199No Hit
GTACTT882970.387321700946606No Hit
GAAGAT871030.38208412649979306No Hit
TATAAG870760.38196568888667415No Hit
AGTTCC867300.38044793280744693No Hit
CTAGTT866520.3801057797028813No Hit
TTCTAC855100.3750963073257787No Hit
CGTCTC854330.37475854079947674No Hit
GGTGGT853390.37434620244269246No Hit
CCGCTC851850.3736706693900884No Hit
TCATGT841840.3692797045481623No Hit
AGGACT826940.36274370293530517No Hit
ACTAAT822730.36089695348630324No Hit
TCCACG819860.3596380055246321No Hit
GTTGGC819700.3595678202724135No Hit
CTTGTA816520.35817288838456884No Hit
GTGGTG812270.3563085926225123No Hit
TGCATA810340.35546198301762544No Hit
GAGTCG805730.35343977043807706No Hit
AGCCTA800330.3510710181756993No Hit
CTCGTC796470.3493777989659256No Hit
CCTGCT790240.3466449607076638No Hit
TAAGTC788180.3457413255853494No Hit
GTCGAG787840.3455921819243849No Hit
ATTGCT781700.3428988228704961No Hit
TGCGGA777210.34092924923011164No Hit
ATGTGA776900.3407932653039381No Hit
TGTGTG772480.3388543977113993No Hit
CGTCAG750800.329344296035779No Hit
ACGTAA739590.3244269418022134No Hit
AGGCTT726740.31879018873340714No Hit
CATACG726490.31868052427681554No Hit
GGCCAG715860.31401759158254233No Hit
GACCGA711790.3122322542292317No Hit
CCGACT706210.309784543558108No Hit
CATGAG703050.3083983848267907No Hit
ACAGCC701860.30787638201341483No Hit
CTATTA692080.30358630847155293No Hit
CATAAC690830.3030379861885951No Hit
GTCTGC688140.3018579966356699No Hit
CACCTA683450.2998006914300122No Hit
GTCTCA648930.2846582232638493No Hit
GCCATA641280.28130249089214754No Hit
GAGCCA635370.278710023138323No Hit
AGATGC629090.275955251988743No Hit
AGTAGG628680.2757754022799328No Hit
TAGAGC627030.2750516168664285No Hit
TACGGC615140.26983597531093384No Hit
CTGTTC604840.2653177996993615No Hit
AAGTTG594940.2609750872183356No Hit
CCACTT593930.2605320428137057No Hit
CAAGGA593890.26051449650065106No Hit
TTGAAT581920.25526376231904707No Hit
CGAAGC574730.2521098125474738No Hit
ATGCCG557340.2444815529469647No Hit
AAGAGG545880.23945453425680746No Hit
GGCTAC527920.23157623969526966No Hit
ACGAAC522990.22941365661128407No Hit
CGTGAA520880.2284880885976513No Hit
TTAGCG519310.2277993958102563No Hit
GCGCAG507010.22240390454595144No Hit
CTATGC497200.21810067126929855No Hit
CGTTGC482780.21177522541309723No Hit
GAATAC476210.208893243493871No Hit
CGCTTG449780.19729951714301106No Hit
CTGAGG434790.19072403632578097No Hit
CCTCGG403470.1769852732039901No Hit
ATGGCC398610.17485339616785014No Hit
CAACGG396830.1740725852369182No Hit
GAGTGA318790.1398397284672962No Hit
TCTCTG311260.13653663503475835No Hit
GTACGC306400.1344047579986184No Hit
GCATAA291540.12788630269881596No Hit
AAGGCG254370.11158139129278251No Hit
GGAGGC248320.1089275114432667No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)