FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25511876
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAC8137433.1896635120051537No Hit
GATTCA7634072.9923593231638472No Hit
ATTCGA6416092.514942452683605No Hit
CGCTTG6122742.399956788752031No Hit
TGATAA5919882.3204408801610668No Hit
CGACAA5836892.287910932147836No Hit
ATTGCT5364182.102620755917754No Hit
GACCAT5309642.0812424770330495No Hit
CTAGTT4784541.8754167666854449No Hit
GCCTGG4754451.8636222596879977No Hit
ATTCAT4581261.7957362288841479No Hit
AGTGCA4500471.7640686243536148No Hit
AATTGC4485871.7583457994229823No Hit
ATCAGC3931421.5410156430675659No Hit
GAACCG3796961.4883107773023043No Hit
GTGGTG3779431.4814394676424423No Hit
TCATGT3381151.3253239393292755No Hit
TATAAG3171801.2432641174643526No Hit
TACTCG3074001.2049290299153226No Hit
TTACGT3017011.1825904139703407No Hit
TTCACA2919721.1444552333195726No Hit
CAGTCC2858011.1202664986298931No Hit
ATACCT2835581.1114745148494765No Hit
TACGAA2833071.1104906593305799No Hit
CTGAGG2774681.087603279351154No Hit
TTCTGT2583211.012551958154704No Hit
GTCTGC2546710.9982448958281234No Hit
TGTGAT2533880.9932158654267526No Hit
TTCCAT2348410.9205163900922065No Hit
CGAAGC2194050.860011235551631No Hit
TCGGAC2183390.8558327894036486No Hit
CCGTCG2144780.8406986612822985No Hit
CTGTAT2093540.8206138976216409No Hit
ATTGAA1982070.7769205212505736No Hit
GCGTAT1947520.7633778088291115No Hit
CTCCGC1918060.7518302456471645No Hit
CCACTT1904320.746444518623405No Hit
GACTAA1882230.7377858061084963No Hit
AGGTCA1844940.7231690840767648No Hit
GACTTG1752360.686880102427591No Hit
AGAACG1716360.6727690272561688No Hit
GCCATA1670730.6548832394763914No Hit
CTTCAC1666070.6530566391903128No Hit
TCTCGC1634440.6406584917549771No Hit
ACTAAT1565720.6137220171499735No Hit
CTACCG1554400.609284867957182No Hit
AGAGGT1512810.5929826563910863No Hit
CCAGCG1468870.5757593051957449No Hit
CCGCTC1446470.5669790806446379No Hit
CGTACT1415570.5548670744558338No Hit
ACCGCT1406000.5511158803060974No Hit
CCTGAC1394350.5465493795909011No Hit
GTCGAG1386560.5434958997135295No Hit
CCGGAA1337660.5243283559390144No Hit
TTAGGC1336190.523752153702848No Hit
GCGGCT1301970.5103387928037907No Hit
ATGTGA1299410.5093353385693784No Hit
CAGATC1249370.4897209440811017No Hit
ACCAGG1229130.4817873840402799No Hit
CAGCCT1227280.48106223156619293No Hit
GATCAG1191330.4669707551102867No Hit
TGACCA1181600.46315684507089955No Hit
TCCTCC1155290.45284400096645183No Hit
GGCAGG1135380.4450397924480348No Hit
TGCGGA1131790.44363260467399573No Hit
GCCAAT1111080.43551481670732484No Hit
GGAGGC1094580.4290472405870897No Hit
TAGCTT1083980.42489231289772655No Hit
GGTAAT1061140.41593961965007986No Hit
CCATGA1055820.4138543163191919No Hit
AACTCT1043490.4090212730729798No Hit
TCTTCA1032000.4045174882474342No Hit
GTACGC992990.38922657040195713No Hit
ACCTGC946300.37092528985324325No Hit
ACTTGA924380.36233321297108845No Hit
ACGCTA913640.3581234088782808No Hit
CGAGCC904720.3546269980302507No Hit
GCACAT903170.35401943784925893No Hit
CATGAG902160.35362354379583844No Hit
CAAGCT889050.3484847605875789No Hit
TCCACG880540.3451490592067788No Hit
AGTTAG878920.3445140608240649No Hit
TACAGT857000.33592198394191003No Hit
TGCGAC855270.33524386838506115No Hit
TTAGCG847950.3323746164335386No Hit
GGCCGT812000.31828313997763236No Hit
TCCAGA809510.3173071239449423No Hit
GACACG804030.3151591047244036No Hit
AACATA803580.3149827162847609No Hit
GCTGTC787000.30848378221970035No Hit
CACATT779440.3055204564337017No Hit
ATCCAC768670.3012988931115846No Hit
AGTTCC760220.29798671018940354No Hit
GCGCAG757560.29694405852395955No Hit
AGGCTT755290.2960542768395394No Hit
GAGAAG745170.2920874968191285No Hit
CGAATT734980.28809327859699535No Hit
ACAGTG729100.28578846965232974No Hit
GAAGAT725830.2845067136575923No Hit
GCTCAA695430.2725906946239469No Hit
CGTCAG681790.26724416503121917No Hit
AATGTA681240.2670285791605447No Hit
TCAATG673800.2641122902917841No Hit
CTTGTA669700.26250519561948327No Hit
TTAGTA665050.2606825150765079No Hit
TCTCTG652050.2555868490423832No Hit
ACACGA642570.2518709325805754No Hit
CGTGAA641550.2514711187840518No Hit
AAGTTG635090.24893896473940214No Hit
CCTGCT601350.2357137515092971No Hit
TGACAC596130.23366764560944087No Hit
TCGTGG595230.2333148687301553No Hit
CGTCTC595120.23327175155602042No Hit
AAGCGC590640.231515706645799No Hit
CAAGGA584730.22919913847182388No Hit
TAAGTC583110.22856414008910986No Hit
NNNNNN578700.22683553338061066No Hit
CAGGAC573210.22468359441696878No Hit
GAGATA568130.222692364920557No Hit
TATTCT555640.21779660578469415No Hit
AGGACT551780.21628358494686947No Hit
GCTTCT537560.21070971025415772No Hit
TAGAGC536240.21019230416453888No Hit
GTTGGC534600.20954946629561855No Hit
CACCTA530050.20776598318367492No Hit
AGATGC529060.20737792861646082No Hit
CGTTGC528120.2070094727647626No Hit
GGACTA527190.20664493665616748No Hit
AGTCAA518560.20326219835812936No Hit
AGGTGT517010.2026546381771376No Hit
CTCGTC516730.20254488537024876No Hit
TCAAGC512500.20088683403760663No Hit
AATCGG503550.197378663960267No Hit
AGTAGG499450.19577156928796613No Hit
TTGAAT498750.19549718727074403No Hit
GAGCCA498310.19532471857420441No Hit
ACTAGC488240.1913775372693094No Hit
GTACTT485960.190483835841786No Hit
ACAGCC482350.18906880858154063No Hit
ACGTAA477190.18704622114030345No Hit
AACCTT468790.18375363693363828No Hit
ATCCGG465080.18229941224236118No Hit
CAGTAG463710.1817624074372265No Hit
GGATTC457560.1793517654287752No Hit
GTAAGA451240.17687448778756998No Hit
CTTAGC435050.17052842370353322No Hit
CATTAA428930.16812954092439145No Hit
TATGGT419760.164535136498782No Hit
CATACG418400.16400205143675048No Hit
CCTCGG417810.1637707865936633No Hit
AAGGCG409740.16060755390940284No Hit
GAGTCG409460.16049780110251397No Hit
ACGAAC400480.1569778717958648No Hit
GTTACA396460.15540213506838932No Hit
GGTACC392970.15403414472538202No Hit
CATAAC377120.1478213519068531No Hit
AAGAGG376410.14754305014652783No Hit
CCTTAT369160.14470123639672755No Hit
CGAGAG353430.1385354804954367No Hit
TACGGC342440.1342276828250498No Hit
GGCTAC333000.13052744533565466No Hit
GTCTCA328330.12869692530647295No Hit
TTCGTT328160.128630289673719No Hit
GAGTGA309940.12148851773973815No Hit
ATGCCG303790.11907787573128686No Hit
GATCCT286280.11221440555763126No Hit
TATTGG284060.11134422258872691No Hit
GACCGA283750.11122271055252855No Hit
GAATAC283730.11121487106632222No Hit
CTGGAG283400.1110855195439175No Hit
AGCCTA282980.11092089033358425No Hit
ATGGCC282270.11064258857325898No Hit
AACTAG264990.10386927249097637No Hit
GAGCAC263830.10341458229100832No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)