FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24686903
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTA8792823.561734738456258No Hit
GAGAAG8770963.552879840780352No Hit
CAGCCT8422143.4115822466673924No Hit
GTACTT8134373.295014364499265No Hit
TACAGT5627702.2796298101872075No Hit
CTTCAC5296382.145420995092013No Hit
ACTTGA4492001.8195883055885949No Hit
GGCCGT4389271.7779751473888807No Hit
AAGCGC4178401.6925573855902458No Hit
GTCGAG3982651.6132643288629602No Hit
TACTCG3955421.602234188711318No Hit
GGTAAT3952711.6011364406462811No Hit
TTGAAT3657501.4815548147128865No Hit
AACTCT3553641.4394839239251678No Hit
AGTGCA3506061.4202105464585817No Hit
TCTCGC3377301.3680533358113005No Hit
CTCGTC3295721.3350074733959136No Hit
TGATAA3049801.2353918999074125No Hit
CAGATC3031031.227788678069501No Hit
TCGTGG3001581.2158592756653195No Hit
GGCAGG2988281.210471803611818No Hit
TAGAGC2770271.1221618199739352No Hit
CGTGAA2687171.0885002464667197No Hit
CTTAGC2584391.0468668346126688No Hit
CTTGTA2494841.0105925396960485No Hit
TTACGT2416990.9790575998941625No Hit
GATCAG2409720.9761127185536395No Hit
AAGGCG2408870.9757684064299196No Hit
CCGTCG2382800.9652081510588834No Hit
ATACCT2245450.9095713625965963No Hit
GACTTG2229020.9029160117816317No Hit
CAAGGA2209000.8948064485853086No Hit
TTCTGT2208990.8948023978544414No Hit
CCTGAC2139940.8668321012157741No Hit
GTCTCA2115050.8567498320870788No Hit
CGTCAG2049550.8302175449063011No Hit
AGGCTT2024650.8201312250467383No Hit
GCCAAT1955040.7919340874795029No Hit
GTACGC1937150.7846873299579133No Hit
AACTAG1849340.7491178622122021No Hit
TGTGAT1829910.7412472921370493No Hit
GGAGGC1808290.7324896120019591No Hit
AGGACT1798570.728552301598949No Hit
CTGTAT1769930.7169510083950182No Hit
CCATGA1729940.7007521356567084No Hit
CCGGAA1519990.6157070410978648No Hit
TGACAC1517210.6145809379167569No Hit
CGTACT1480290.5996256395547064No Hit
TTCTAC1434770.5811867126467828No Hit
AGGTGT1434350.5810165819503564No Hit
GCCATA1347850.5459777599482608No Hit
CACCTA1346220.5453174908168918No Hit
GTCTGC1345640.5450825484265888No Hit
ATTCAT1345500.5450258381944467No Hit
AGAACG1333910.5403310411192526No Hit
TAGCTT1326850.5374712251269428No Hit
TTCCAT1315200.5327521236665449No Hit
GATTCA1291250.5230506232393751No Hit
CCAGCG1285950.5209037358797092No Hit
TTAGTA1276330.5170069327853721No Hit
AATGTA1264100.5120528889346712No Hit
TCAATG1258100.5096224504142945No Hit
TCATGT1252570.5073823962446808No Hit
ATTGAA1252490.5073499903977424No Hit
CTCCGC1247160.5051909508454746No Hit
AAGTTG1170130.4739881709747067No Hit
CGTTGC1129980.4577244865425201No Hit
CCGACT1124480.4554965845655083No Hit
GGCTAC1114670.4515228175846926No Hit
CATTAA1109260.44933137218548636No Hit
ACCTGC1091360.4420805639330296No Hit
ACAGTG1066160.431872722147448No Hit
ATGTGA1063230.4306858580033307No Hit
AGTTAG1055770.4276640127763292No Hit
TTCACA1054050.4269672870671546No Hit
GCACAT1040940.42165677890013176No Hit
GAGTGA1033470.4186308829422629No Hit
GACCAT1015980.4115461546553652No Hit
ACGTAA1008820.4086458313543825No Hit
AATTGC1008140.4083703816554065No Hit
CAACGG970820.39325305405866423No Hit
AGATGC936090.37918486575655114No Hit
CTGAGG919980.37265913832934006No Hit
GCTTCT904990.36658709275926593No Hit
GAGCAC904440.36636430256156477No Hit
AAGAGG899100.36420121227842955No Hit
ATTCGA886560.3591215957708425No Hit
TATGGT874160.35409868949539763No Hit
CCTGCT847690.34337640488966964No Hit
ACTAAT836300.3387626224318214No Hit
TCCAGA833450.33760816413464256No Hit
TAAGTC828570.33563140747140296No Hit
CCTCGG803550.3254964788414326No Hit
GTGGTG802290.32498608675215357No Hit
TCGGAC789350.31974444100987476No Hit
GAGTCG789110.3196472234690597No Hit
TACGAA771380.3124652776413469No Hit
GTTACA745810.3021075588136754No Hit
TGCGAC736720.2984254444553049No Hit
ACAGCC698440.28291924669530233No Hit
AACCTT683550.2768877084339012No Hit
TCTTCA674520.2732298984607344No Hit
TCTCTG668690.2708683223651019No Hit
AGCCTA661630.2680085063727921No Hit
ATTGCT659070.26697151927076473No Hit
GCGCAG649130.26294509278867423No Hit
ATCCGG641360.25979767490478656No Hit
GACCGA640850.25959108763055455No Hit
CAGTCC627300.2541023473053708No Hit
CAAGCT625910.25353929571481687No Hit
CGAATT611290.24761712718683263No Hit
AGTTCC585270.23707712547013288No Hit
CGAAGC563850.2284004599523885No Hit
TGACCA560000.2268409285684802No Hit
GGCGTT557460.22581204292818746No Hit
GCTCAA510810.2069153834322596No Hit
GGCCAG502360.20349251584939593No Hit
GAGATA495570.20074206959050314No Hit
GGATTC492880.19965242298720093No Hit
CACATT479710.19431761043497436No Hit
CAGTAG470500.19058688730619633No Hit
AGAGGT465950.18874380476157743No Hit
GAATAC447150.18112843073106416No Hit
CCACTT433460.17558298017373827No Hit
ATGCCG430250.17428269556533682No Hit
ATCCAC428770.17368318739697725No Hit
TCAAGC417920.16928814440596296No Hit
TCCTCC409430.1658490738996301No Hit
AAGCAT406290.1645771444072997No Hit
TTCGTT405130.16410725962669354No Hit
AGTCAA404480.16384396212031943No Hit
TTAGCG399330.16175783572366287No Hit
ACTAGC381210.15441791139212563No Hit
CGTCTC373330.15122593546869773No Hit
GCTGTC361100.1462718916179968No Hit
ACACGA335360.1358453103655813No Hit
GACACG332650.13474756230054455No Hit
ACCAGG317330.1285418426118497No Hit
TTAGGC316360.12814892171772216No Hit
CTAGTT306630.1242075605838448No Hit
GTAAGA301060.12195130349076189No Hit
CATAAC300020.12153002748056328No Hit
TACGGC285820.11577798964900539No Hit
CGAGCC283790.11495569128294465No Hit
TGCATA282930.11460732842835733No Hit
AACATA262470.10631953307387322No Hit
CTGGAG255040.10330984003947356No Hit
GGACTA254480.10308299911090507No Hit
GATCCT253230.1025766577524933No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)