FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001729941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17554644
Sequences flagged as poor quality0
Sequence length6
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATAA7474244.2576995580200885No Hit
CTGTAT3810882.170867150595592No Hit
ATTCAT3415521.9456503931381346No Hit
TTCTAC3377451.9239638240456485No Hit
TGTGAT3357811.912775901351232No Hit
CGAATT3190111.8172456245766078No Hit
GACCAT2691151.5330131445559363No Hit
TGCGAC2648231.5085637737797475No Hit
CGTACT2641031.5044622949915705No Hit
CACATT2628791.4974897810516692No Hit
AGTGCA2575631.4672071959989619No Hit
GATTCA2573141.4657887679180506No Hit
ACTAAT2409531.3725883589550434No Hit
CTAGTT2392721.3630125452843134No Hit
ATTCGA2344111.3353218669658011No Hit
CCGGAA2307201.3142960916780768No Hit
AGGTGT2271291.2938399662220437No Hit
ATACCT2137431.2175866397518513No Hit
TCTTCA2135011.2162080871591585No Hit
TCAAGC2113701.2040688492458178No Hit
GGTAAT2010791.1454461850664701No Hit
AAGCGC1944631.1077581522017763No Hit
CATACG1935181.102374961292294No Hit
AATCGG1896911.0805744622334694No Hit
TAAGTC1825831.0400837521968547No Hit
TATTCT1703700.9705124182524009No Hit
TTCACA1703400.9703415233028935No Hit
TTCCAT1615860.9204743770366406No Hit
TCATGT1565810.8919634029604929No Hit
AGTCAA1561400.8894512472027345No Hit
ATTGCT1482600.8445628404654631No Hit
GAAGAT1424040.811204146321623No Hit
CCGTCG1386070.7895745422123058No Hit
CTTCAC1314110.7485825403238026No Hit
GGACTA1299520.7402713492794272No Hit
ACCAGG1290860.7353381817369808No Hit
GTCGAG1290080.7348938548682616No Hit
CAGTCC1281610.7300689207938367No Hit
CTGAGG1272370.7248053563490094No Hit
CAAGGA1267650.7221166091434267No Hit
CGACAA1261410.7185619941936732No Hit
TGACCA1253180.7138737760788542No Hit
TCTCGC1207900.6880800316998739No Hit
CTCCGC1175120.6694069102170342No Hit
ATTGAA1173200.6683131825401871No Hit
TGCATA1145380.6524655242225362No Hit
ATCCGG1137750.6481190960067319No Hit
TACTCG1113150.6341057101471269No Hit
ATCCAC1080600.6155636081255763No Hit
CGCTTG1072800.6111203394383845No Hit
TCCTCC1070430.6097702693372762No Hit
AGGTCA1067920.6083404482597312No Hit
GACTAA1066370.6074574910206098No Hit
CTGTTC1066000.6072467205828839No Hit
GAGATA1033490.588727404554601No Hit
AGAACG1030970.5872918869787391No Hit
GTAAGA1023950.5832929451602664No Hit
TCCAGA1023400.5829796377528362No Hit
GATCCT1020780.5814871551938051No Hit
GGCCGT996200.567485162330834No Hit
CTTGTA996140.5674509833409325No Hit
CCAGCG992740.5655141739131821No Hit
GAACCG953990.5434402429351458No Hit
ACTTGA931480.5306174252237755No Hit
TATAAG924630.5267153238766904No Hit
TACAGT913580.520420693236502No Hit
AATTGC911090.5190022651555907No Hit
GGATTC900260.5128329574783744No Hit
GCGTAT890190.5070965836732434No Hit
CTTAGC876750.49944048993531287No Hit
TAGAGC870560.49591435747714396No Hit
TGACAC861410.49070206151716894No Hit
AACTCT859760.48976213929487833No Hit
CGAGCC857230.4883209252206994No Hit
ACGAAC857090.4882411742442626No Hit
GATCAG839890.47844319713917294No Hit
ACCGCT835670.47603927484943587No Hit
TGGCAT834720.4754981075093292No Hit
GACACG823160.46891295545497824No Hit
AACCTT819070.46658308764336087No Hit
GCCATA800620.45607304824865713No Hit
GGCTAC757660.43160089147920055No Hit
CTGGAG751070.4278468990883552No Hit
CTACCG737150.4199173734312128No Hit
GACTTG734080.4181685484479207No Hit
AAGCAT719640.40994280487829887No Hit
TCCACG714570.40705468023162417No Hit
GGCAGG707990.40330638433909566No Hit
ACACGA700600.39909667208289723No Hit
AACATA675500.3847984613074466No Hit
TCGGAC640740.3649974331578584No Hit
AGAGGT628510.3580306157162743No Hit
TATTGG626520.35689701255120865No Hit
AGTTCC598340.340844280294149No Hit
AGTTAG561410.3198071120097907No Hit
TAGCTT560920.319527983592262No Hit
ACAGTG560230.319134925208395No Hit
GCGCAG558490.3181437345012522No Hit
TGCGGA555490.3164347850061784No Hit
GGTACC550980.31386566426525087No Hit
CAGATC535000.3047626599548245No Hit
CCACTT530330.3021023952408263No Hit
GTACTT521410.29702111874214027No Hit
TACGAA503720.28694401321952184No Hit
CTATTA497530.28341788076135294No Hit
GTCTGC494410.2816405732864762No Hit
CAGGAC493680.2812247289093416No Hit
TTAGGC486170.27694665867334023No Hit
TTCTGT480980.2739901760468626No Hit
GAGCCA473330.26963235483442444No Hit
NNNNNN473120.2695127283697693No Hit
CCTGAC459740.2618908136217402No Hit
GTTGGC455010.25919636991784056No Hit
CCGCTC444990.25348847860429413No Hit
TAATGA436480.24864075853660147No Hit
GCGGCT423630.24132075819936877No Hit
CGTCTC404080.23018410398980466No Hit
GTCTCA394460.2247040726089347No Hit
TTAGCG392910.22382111536981328No Hit
GCTGTC388740.2214456755716607No Hit
CATGAG340730.19409678715216327No Hit
GCCAAT319350.1819176737506041No Hit
GTGGTG317970.18113155698287017No Hit
GCCTGG279890.15943929139206697No Hit
GCTCAA266130.15160090970799522No Hit
CTATGC262660.14962422479202653No Hit
ATGTGA261990.1492425594047934No Hit
GCACAT261290.1488438045226095No Hit
GGATCG249270.14199661354568058No Hit
CGAGAG181470.10337435495701308No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)