FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21828583
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACA7134403.2683752307696747No Hit
TGCGAC6001762.7494959246782074No Hit
TTAGTA5014112.2970387037949282No Hit
CCGGAA4511722.066886338888786No Hit
CACCTA4464562.045281638299655No Hit
ATACCT3383311.5499448589951992No Hit
CACATT3341641.5308552094288486No Hit
GCACAT3156821.4461864061446406No Hit
CTAGTT2818391.2911465668660214No Hit
GTAAGA2747691.2587578405799404No Hit
CCTGCT2627791.2038298592263181No Hit
TCGTGG2611931.1965641562716187No Hit
GCGCAG2587021.185152513106325No Hit
AGATGC2527561.1579129987503083No Hit
ACCTGC2451551.1230916821307182No Hit
TTCCAT2337961.0710544060510019No Hit
AGGCTT2263741.037053115174723No Hit
CATTAA2162090.9904857314833492No Hit
CCAGCG2151880.9858083779418939No Hit
TATTCT2141950.9812592965837499No Hit
ATTCAT2137410.9791794547543466No Hit
TCCAGA2129340.9754824671853414No Hit
AGGTCA2121100.9717076000764685No Hit
ACAGCC2113480.968216764230642No Hit
GGCCGT2107100.965293990910908No Hit
GTCTGC2049620.9389615441368777No Hit
TACTCG1972690.9037187617721223No Hit
GTACTT1926480.8825492703763685No Hit
CAGTCC1904370.8724203490441867No Hit
CCATGA1896070.86861799503889No Hit
CCGTCG1894140.8677338332039234No Hit
CTTAGC1883770.8629831812720047No Hit
GGACTA1873340.858205042443662No Hit
GACTTG1805350.8270578076460575No Hit
CTACCG1777810.814441322187519No Hit
TATGGT1770430.8110604339273878No Hit
TGATAA1736380.7954616202068635No Hit
CTGTAT1701500.7794825710858098No Hit
AGGACT1693080.7756252432876656No Hit
AGAACG1687120.7728948782428983No Hit
CGAATT1646750.7544007780990639No Hit
GTGGTG1633980.7485506503101919No Hit
GCTCAA1621500.7428333758540351No Hit
ATTGAA1612870.7388798439184074No Hit
GTCTCA1572990.7206102200953677No Hit
AACTAG1565750.7172934679268921No Hit
CGTACT1530860.7013098376564342No Hit
AAGAGG1506860.6903150790869018No Hit
AACTCT1477730.6769701908731318No Hit
TTCTGT1466940.6720271306662462No Hit
AATGTA1393130.6382136669155299No Hit
TCATGT1375110.6299584356895727No Hit
AGCCTA1374410.6296377552312947No Hit
GACCAT1372670.6288406352350037No Hit
GAGATA1368910.6271181230591102No Hit
GGATTC1357640.6219551676808339No Hit
CTTCAC1336770.6123943088747447No Hit
TAGAGC1334360.6112902518683875No Hit
AGTTAG1331700.610071666126931No Hit
TCTTCA1311260.6007077967452125No Hit
CAGATC1301050.5960304432037572No Hit
ACTAAT1257560.5761070244458836No Hit
ATGTGA1253530.574260821236083No Hit
TTCGTT1217510.5577595210829763No Hit
ATTCGA1210950.5547542870739709No Hit
GGTAAT1208310.5535448636313223No Hit
TAAGTC1206910.5529035027147663No Hit
AGTGCA1176130.5388027248493409No Hit
TAGCTT1162940.532760188785502No Hit
TCTCGC1139240.5219028646980888No Hit
CCTTAT1131470.5183433116112026No Hit
GCCAAT1101510.504618187996903No Hit
ATGGCC1085930.4974807572255148No Hit
ACTAGC1070820.49055864047611336No Hit
CTGGAG1069200.4898164942726699No Hit
ATCCAC1055490.4835357384398245No Hit
CTGAGG1040220.4765403232999595No Hit
AACCTT1035470.47436427733307285No Hit
GATCAG1024010.46911428011612116No Hit
AGGTGT987350.45231978640116033No Hit
TACAGT980870.4493512015873866No Hit
AAGCAT975760.44701023424195696No Hit
GGCGTT964060.4416502894393099No Hit
AGTCAA958360.43903903427904595No Hit
GTCGAG954740.4373806581948082No Hit
AATCGG939960.4306097193757377No Hit
CGTTGC920300.42160317964752914No Hit
AGAGGT912060.41782831253865627No Hit
GAGTCG899950.4122805406104464No Hit
AAGTTG876990.40176222157892705No Hit
TACGGC875880.4012537139950862No Hit
TCAATG870980.39900895078714No Hit
GCTTCT855620.3919723053026392No Hit
GCCATA842490.38595725613522414No Hit
ATTGCT841280.3854029370573436No Hit
AATTGC840900.38522885337999263No Hit
GACCGA831550.380945478687279No Hit
ACAGTG823970.37747296743906833No Hit
CCTCGG818910.3751549058406585No Hit
CAAGGA790700.36223148337205396No Hit
GATTCA765180.3505403900931178No Hit
GAGCAC748360.34283489679563717No Hit
GAGTGA743550.34063136393232674No Hit
TTCTAC720470.33005807110795965No Hit
TGACAC699190.3203093851763076No Hit
ATGCCG693140.3175377897869046No Hit
CTGTTC688060.31521056588968693No Hit
GGCCAG682930.31286043624544935No Hit
ACGTAA682890.3128421116478335No Hit
ATCAGC658100.30148544227538726No Hit
TACGAA654640.299900364581613No Hit
TCGGAC643340.2947236657551249No Hit
CTCCGC632760.2898768096857226No Hit
CGCTTG628510.2879298211890346No Hit
CAACGG623610.2856850579810884No Hit
CAGTAG610360.2796150350208257No Hit
CAGGAC609230.27909736513817685No Hit
GTTACA604570.27696254951592597No Hit
CCACTT600090.2749101945829466No Hit
AGTAGG596960.2734762948195034No Hit
CCTGAC592410.2713918718406962No Hit
TGACCA589710.2701549615016238No Hit
TGGCAT577190.2644193624478511No Hit
ACGAAC569760.26101556844069995No Hit
TGCATA568390.26038795097235584No Hit
AAGCGC565500.25906399879460795No Hit
GCCTGG561970.2574468530550059No Hit
TAATGA545460.24988337538904837No Hit
CCGCTC542240.24840824528096944No Hit
GCTGTC539550.24717591609130105No Hit
GGCAGG538800.24683232988600315No Hit
AACAAT532070.24374921633713006No Hit
GAATAC526280.24109673083223038No Hit
ACCGCT525460.2407210765811047No Hit
TGCGGA481180.22043574702031735No Hit
GCATAA463580.21237292406932692No Hit
ACTTGA452450.20727410478270625No Hit
GAAGAT448780.2055928229514486No Hit
CCGACT445630.20414976088919742No Hit
TGTGTG441640.20232188227701264No Hit
AACATA431800.19781403126350436No Hit
GAGCCA428160.19614649288045863No Hit
CTATGC422400.19350775082377084No Hit
TCAAGC422180.19340696553688347No Hit
TTAGCG421570.19312751542324116No Hit
ATCCGG412760.18909152279834196No Hit
CATGAG408800.18727738763436913No Hit
AGTTCC408630.18719950809450162No Hit
TCCACG400750.1835895623641718No Hit
CGAGCC382170.17507778677159208No Hit
TCCTCC371240.17007059047305087No Hit
CATACG370900.1699148313933158No Hit
TATAAG365590.16748224105980677No Hit
ACGCTA348230.15952936569451165No Hit
TGTGAT339660.15560332065530777No Hit
GGCTAC327730.15013800941636937No Hit
GGTGGT327300.14994101999199855No Hit
CAGCCT325220.14898814091597243No Hit
CGACAA322900.14792531425425096No Hit
GCGTAT300740.13777348717504934No Hit
CGTCTC295340.13529966649690453No Hit
CTCGTC287580.1317446945594224No Hit
GACTAA274730.12585791757531856No Hit
GGTACC273160.12513867711889498No Hit
CGAAGC269680.12354443712631277No Hit
AGACGG267400.1224999350622072No Hit
ACACGA265790.12176237000816773No Hit
TTACGT253480.1161229750918784No Hit
TATTGG250710.11485399670697817No Hit
GACACG250620.11481276636234243No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)