FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23880820
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCT7030792.9441158218185137No Hit
CCATGA4089911.7126338207816985No Hit
CCTGCT4053661.697454275020707No Hit
CACCTA4016251.681788983795364No Hit
TTACGT3804541.593136249090274No Hit
AGGCTT3744321.5679193595529801No Hit
TTAGTA3709261.5532381216390392No Hit
AGATGC3563351.4921388796532111No Hit
CGTGAA3449221.4443473884062608No Hit
TCGTGG3383091.416655709477313No Hit
ACCTGC3378311.4146541031673117No Hit
GCACAT2883731.2075506619956937No Hit
ATCCGG2533261.0607927198479785No Hit
CATAAC2493951.0443318110517141No Hit
AAGAGG2457221.0289512671675427No Hit
CGTCAG2360860.9886008939391528No Hit
GGCGTT2356210.9866537246208463No Hit
GAGCCA2244720.939967723051386No Hit
AGCCTA2244540.9398923487551935No Hit
TAAGTC2218710.9290761372515685No Hit
GTACGC2116810.886405910684809No Hit
CCTTAT2106560.8821137632627355No Hit
GAGAAG2099570.8791867280939265No Hit
CAGCCT2026120.848429827786483No Hit
GGTACC2001810.8382501103395947No Hit
AACAAT1985810.8315501729002606No Hit
CCTCGG1971720.8256500404927468No Hit
TCAAGC1929130.8078156445214193No Hit
GAATAC1887990.7905884303805313No Hit
TTGAAT1877020.7859947857736879No Hit
GGAGGC1867610.7820543850671794No Hit
CCGACT1811890.7587218529346983No Hit
CAGTAG1791420.75015012047325No Hit
AATGTA1781450.7459752219563649No Hit
ACTAGC1740720.72891969371236No Hit
AGGACT1734980.7265160911559989No Hit
CATACG1728500.7238026164930685No Hit
TAATGA1727750.7234885569255998No Hit
CTCGTC1724420.7220941324460383No Hit
CTGTTC1720710.7205405844522927No Hit
CGAGCC1691550.7083299484691062No Hit
AACCTT1683480.704950667523142No Hit
CATTAA1654090.6926437199392651No Hit
TGATAA1651700.6916429167842645No Hit
GAAGAT1648430.6902736170701006No Hit
GACCGA1623330.6797630902121451No Hit
CTAGTT1621680.6790721591637138No Hit
AAGTTG1617520.6773301754294869No Hit
ATTGAA1594860.6678413890310299No Hit
AAGGCG1548150.6482817591690737No Hit
GGCCAG1527360.6395760279588389No Hit
TGCATA1521430.6370928636453858No Hit
CGTTGC1519510.6362888711526656No Hit
ACGCTA1500820.6284625067313434No Hit
ATGGCC1484180.6214945717944359No Hit
GATCCT1441740.603722987736602No Hit
TATTGG1412270.5913825404655284No Hit
TCAATG1388350.5813661339937238No Hit
GGTGGT1374030.5753696899855197No Hit
GCTTCT1367060.5724510297385098No Hit
AAGCAT1329610.5567689886695683No Hit
ACTAAT1299950.5443489796414026No Hit
ACGAAC1293280.5415559432213801No Hit
ACACGA1271120.5322765298679023No Hit
GAGCAC1206960.5054097807361724No Hit
TTCACA1204230.504266603910586No Hit
ATCAGC1204200.5042540415278872No Hit
CTATTA1202760.5036510471583471No Hit
TTCGTT1200310.5026251192379492No Hit
TACGGC1191190.49880615489752866No Hit
GGATCG1188410.49764204076744434No Hit
CGAGAG1176890.49281808581112374No Hit
ATGCCG1163150.4870645145350955No Hit
TCCACG1131900.47397869922389596No Hit
GACCAT1128500.4725549625180375No Hit
TGTGTG1059530.44367404469360766No Hit
ATCCAC1057950.44301242587147344No Hit
CAGGAC1053640.44120763022375276No Hit
CTTCAC1051460.44029476374764354No Hit
AGTGCA1041140.43597330409927293No Hit
AGACGG1031600.43197846640106996No Hit
CCGCTC1030540.4315345955457141No Hit
TCTCTG1030430.43148853347581867No Hit
GTCTCA973520.40765769349628694No Hit
AACTAG959090.4016151874181875No Hit
ACTTGA952550.3988765879898597No Hit
TAGCTT942920.39484406314356035No Hit
ATACCT939500.39341195151590275No Hit
GGATTC935180.3916029684072825No Hit
ACAGCC920510.385459963267593No Hit
GTACTT919730.38513334131742544No Hit
ACGTAA915810.3834918566447886No Hit
GTTACA911410.3816493738489717No Hit
GCTGTC911360.38162843654447376No Hit
GGCTAC904980.3789568364905393No Hit
AGTAGG902980.3781193443106225No Hit
AACATA900230.3769677925632369No Hit
CTTGTA899890.3768254188926511No Hit
CTGTAT873090.3656030236817664No Hit
ACCAGG866270.3627471753482502No Hit
TCCTCC863540.3616039985226638No Hit
TCATGT859770.3600253257635207No Hit
CGAATT856610.3587020881192522No Hit
TACAGT846160.35432619147918704No Hit
TGCGAC845540.35406656890341287No Hit
AGTTCC841050.3521863989594997No Hit
CTTAGC840640.3520147130626168No Hit
ATTCAT818630.34279811162263274No Hit
GAGTCG812460.3402144482475895No Hit
GGACTA810930.33957376672995315No Hit
CTGAGG808840.33869858740194014No Hit
ACCGCT797670.33402119357710497No Hit
CACATT792240.3317474023086309No Hit
GCCAAT779920.3265884504803437No Hit
GACACG775190.3246077814748405No Hit
GTCTGC767750.3214923105655501No Hit
AGTCAA765800.32067575569013124No Hit
CAACGG763390.3196665776133315No Hit
TTCTGT761940.3190593957828919No Hit
GATCAG751630.3147421235954209No Hit
ACAGTG745020.311974211940796No Hit
TATGGT744250.31165177745152806No Hit
TAGAGC743470.3113251555013605No Hit
GAGATA741290.31041228902525125No Hit
GGCCGT733720.3072423811242662No Hit
GCGCAG729180.3053412738758552No Hit
GCGTAT727830.30477596665441137No Hit
AAGCGC726210.30409759798867875No Hit
GTTGGC722680.3026194242911257No Hit
TACTCG722160.30240167632434733No Hit
TCTTCA710780.29763634582062093No Hit
AGGTCA709370.29704591383377954No Hit
CGTACT696230.2915435902117264No Hit
CAGATC690500.28914417511626483No Hit
GCTCAA686600.28751106536542714No Hit
GGCAGG682260.28569370733500776No Hit
TTAGGC677590.2837381630949021No Hit
GTAAGA669950.28053894296762005No Hit
CCAGCG648180.271422840589226No Hit
AACTCT647350.27107528133456055No Hit
TATTCT638510.2673735658993284No Hit
GAGTGA634510.2656985815394949No Hit
CATGAG632530.26486946428137725No Hit
CCGGAA631240.26432928182533094No Hit
GTGGTG622060.260485192719513No Hit
TCTCGC607510.2543924371106185No Hit
CAAGGA600100.2512895285840268No Hit
TCGGAC591050.24749987646990346No Hit
AATCGG591020.2474873140872047No Hit
TATAAG587640.24607195230314538No Hit
GCATAA586830.24573276797027907No Hit
ATGTGA586000.24538520871561365No Hit
GATTCA582960.24411222060214013No Hit
TGACCA579250.2425586726083945No Hit
AATTGC577900.2419933653869507No Hit
GACTAA577450.2418049296464694No Hit
TGACAC575880.24114749828523474No Hit
CCTGAC567190.2375085947634964No Hit
AGAGGT556590.2330698862099375No Hit
GTCGAG549310.23002141467504048No Hit
AGAACG544120.22784812246815644No Hit
ATTCGA530710.2222327374018145No Hit
GCGGCT526490.22046562890219012No Hit
CTCCGC522370.21874039501156156No Hit
CAGTCC517450.21668016424896633No Hit
TTCCAT515930.2160436701922296No Hit
CGAAGC512550.2146283084081702No Hit
ATTGCT512200.21448174727668481No Hit
GGTAAT511920.21436449837149646No Hit
TGTGAT510450.21374894161925762No Hit
CCACTT485880.20346035018898012No Hit
GACTTG485380.20325097714400092No Hit
CTGGAG480830.20134568243469025No Hit
AGTTAG479820.20092274888383227No Hit
GCCTGG465110.19476299390054447No Hit
TCCAGA464580.1945410584728665No Hit
TTCTAC453320.1898259774999351No Hit
CGACAA451570.18909317184250793No Hit
TACGAA439960.18423152973809107No Hit
CTACCG437590.1832391015048897No Hit
GCCATA427670.17908514029250253No Hit
CGTCTC407210.17051759529195396No Hit
AGGTGT400790.16782924539442112No Hit
CCGTCG368140.15415718555727986No Hit
TTAGCG346800.14522114399756791No Hit
GAACCG321180.1344928691728341No Hit
CGCTTG301990.1264571317065327No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)