FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001729949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409659
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTA314622.231887286216028No Hit
GGCTAC278501.9756551052417641No Hit
TAGCTT271651.9270617929584388No Hit
ACTTGA266631.8914503436646735No Hit
GATCAG240551.7064410612779402No Hit
GAAGAT236811.679909822162665No Hit
CAGATC231291.6407514157679266No Hit
GCCAAT208891.4818477376443522No Hit
CGAGAG208391.4783007805433797No Hit
ACAGTG197921.4040274988490125No Hit
TGACCA197821.403318107428818No Hit
TTAGGC193771.3745877549109395No Hit
GTACTT176641.2530690046316164No Hit
ACACGA168641.1963176910160542No Hit
CTATTA159041.1282161146773793No Hit
CTTCAC156761.112041990296944No Hit
TAGAGC155591.1037421106806682No Hit
GTCTCA148091.0505377541660785No Hit
GGTACC144071.0220202190742584No Hit
ATTGAA143851.0204595579498303No Hit
AACTCT140260.9949924059648467No Hit
CAGGAC134420.9535639470254863No Hit
GATCCT130970.929089943028775No Hit
TGATAA128290.9100782529675616No Hit
ATCAGC122620.8698557594425319No Hit
AGTTCC118810.8428279463331203No Hit
GAGCCA117510.8336058578705914No Hit
CGAGCC117110.8307682921898133No Hit
ATTCAT116700.8278597873670157No Hit
GGCAGG115670.820553055739012No Hit
TATAAG114060.8091318538738802No Hit
TTAGCG113460.804875505352713No Hit
TATTGG112490.797994408576826No Hit
TACAGT112190.7958662343162424No Hit
CCGCTC110740.7855800587234217No Hit
GACCGA108950.7728819523019397No Hit
TGACAC107690.7639436204074885No Hit
CCACTT107240.7607513590166133No Hit
TCCACG104190.7391149207006801No Hit
TGCGAC102630.7280484145456454No Hit
TTCTGT100730.7145699775619494No Hit
GGATCG100540.7132221338635798No Hit
ACTAAT100210.7108811421769379No Hit
TCGGAC96270.6829311202212733No Hit
CGTACT95900.6803063719665536No Hit
CTAGTT95030.6741346666108612No Hit
AGTGCA94970.6737090317587445No Hit
CCTGCT94100.6675373264030521No Hit
CAGTCC92760.6580314813724454No Hit
CTGTAT90850.6444821052467299No Hit
CCATGA89550.635260016784201No Hit
GTCTGC87800.6228456669307967No Hit
ACCAGG87290.6192277706878047No Hit
GACCAT86920.6166030224330848No Hit
ATACCT86170.611282586781626No Hit
TCCTCC84190.5972366366617742No Hit
CAAGGA83380.5914905661581985No Hit
CCGTCG81720.5797146685829694No Hit
TTCCAT79190.5617670656520478No Hit
GCGCAG78430.5563756908585693No Hit
AGTTAG78150.5543893948820247No Hit
AGGACT77830.5521193423374021No Hit
GACTAA75680.5368674268032197No Hit
TCAAGC74080.5255171640801073No Hit
AGGTCA73850.5238855638136599No Hit
CCTGAC73420.5208351807068234No Hit
GCCATA73170.5190617021563371No Hit
TCTTCA73070.5183523107361426No Hit
GCGTAT72950.5175010410319091No Hit
GAACCG72400.5135993882208393No Hit
CCGGAA71950.5104071268299638No Hit
GTGGTG71340.5060798391667772No Hit
AACATA71270.5055832651726411No Hit
GTTGGC70700.5015397340775323No Hit
CGACAA70220.49813465526059847No Hit
CTACCG69560.49345267188731456No Hit
TGTGAT69130.4904022887804781No Hit
GACACG66800.4738734686899456No Hit
TACGAA65820.46692143277203924No Hit
CGAAGC64720.4591181271498994No Hit
ATTGCT64170.45521647433882945No Hit
AGATGC63600.45117294324372065No Hit
AATGTA63480.45032167353948716No Hit
AATCGG62400.4426602462013863No Hit
TCATGT62080.4403901936567638No Hit
ATGTGA61440.43585008856751883No Hit
TGCGGA61100.43343815773885747No Hit
CAAGCT59760.4239323127082507No Hit
GACTTG59670.42329386043007566No Hit
ATTCGA58390.41421365025158563No Hit
CATAAC56820.4030762049545316No Hit
CCAGCG56600.40151554383010357No Hit
AACCTT56280.3992454912854811No Hit
AGAACG56270.39917455214346165No Hit
CATGAG55940.39683356045681967No Hit
TTCTAC55190.39151312480536077No Hit
ACTAGC54640.3876114719942908No Hit
GCTGTC54610.38739865456823247No Hit
TTAGTA53230.37760905296954794No Hit
TTCACA53170.3771834181174312No Hit
CATTAA49690.3524965966946616No Hit
ACCTGC47750.3387344031428877No Hit
AATTGC46140.3273132012777558No Hit
AAGTTG45920.3257525401533279No Hit
TATTCT45910.3256816010113084No Hit
AGGCTT45900.32561066186928894No Hit
CACCTA45900.32561066186928894No Hit
CGCTTG45480.32263121790447197No Hit
GCACAT45420.3222055830523552No Hit
GATTCA44750.3174526605370519No Hit
AAGAGG44730.31731078225301296No Hit
GCTCAA43230.30666991095009505No Hit
TAAGTC43170.3062442760979783No Hit
TAATGA43120.30588958038788105No Hit
TCAATG42880.3041870409794142No Hit
CGAATT42730.30312295384912236No Hit
CAGCCT42110.2987247270439163No Hit
GTACGC41560.29482307423284637No Hit
TGCATA40890.290070151717543No Hit
GAGAAG40780.2892898211553291No Hit
TACTCG40780.2892898211553291No Hit
GTAAGA39180.27793955843221657No Hit
AAGCAT38480.2729738184908549No Hit
GAGATA38010.2696396788159406No Hit
TGTGTG37370.2650995737266956No Hit
GCCAAA37340.26488675630063724No Hit
CGTTGC37310.2646739388745789No Hit
ATCCAC37250.26424830402246213No Hit
TCGTGG36860.2614816774837035No Hit
CTCCGC36740.26063040777947005No Hit
GAGCAC36040.2556646678381084No Hit
ATCCGG34360.24374689197884028No Hit
TATGGT34120.2420443525703734No Hit
CTGAGG31900.22629586304205485No Hit
AGTCAA31890.22622492390003537No Hit
TTCGTT31450.22310360165117946No Hit
CCGACT31230.2215429405267515No Hit
AGAGGT31140.22090448824857645No Hit
CTGTTC30680.2176412877156816No Hit
CTGGAG30600.21707377457952595No Hit
GCGGCT30280.21480372203490347No Hit
GGCGTT30200.21423620889874784No Hit
GCCTGG29910.21217897378018372No Hit
GGATTC29870.2118952172121059No Hit
CACATT29720.21083113008181412No Hit
GGACTA29540.20955422552546396No Hit
TTACGT28850.20465942472612172No Hit
AGCCTA27600.19579203197369008No Hit
CGTCTC27240.19323822286098977No Hit
ACGAAC27120.19238695315675636No Hit
GGTAAT26210.18593149123298613No Hit
CCTTAT26120.18529303895481106No Hit
TCTCTG25570.18139138614374115No Hit
CTTAGC24900.1766384636284378No Hit
TCTCGC24630.1747231067939126No Hit
GAATAC24360.17280774995938733No Hit
ACCGCT23890.16947361028447305No Hit
AAGCGC23690.16805482744408398No Hit
ACGCTA23570.16720355773985057No Hit
CAGTAG23530.16691980117177274No Hit
AGACGG22070.1565626864369326No Hit
GGTGGT22040.15634986901087428No Hit
GCTTCT21880.155214842738563No Hit
AGGTGT20480.1452833628558396No Hit
AAGGCG20220.14343894516333383No Hit
GTCGAG19820.14060137948255572No Hit
GTTACA19780.1403176229144779No Hit
GGCCGT19470.13811850951187488No Hit
CCTGCC19320.13705442238158308No Hit
ATGCCG19200.13620315267734961No Hit
TCCAGA19070.13528094383109673No Hit
CATACG18520.13137929102002682No Hit
CTCGTC18330.13003144732165722No Hit
ATACCC18100.12839984705520982No Hit
GGAGGC17830.12648449022068456No Hit
ATGGCC17640.125136646522315No Hit
TTGAAT17340.12300847226173138No Hit
ACGTAA17270.12251189826759522No Hit
GGCCAG17260.12244095912557576No Hit
AGTAGG16840.11946151516075874No Hit
CGTCAG16420.11648207119594173No Hit
CGTGAA16160.11463765350343594No Hit
AACAAT15920.11293511409496908No Hit
CAACGG15380.10910440042591861No Hit
ACCGCC15220.10796937415360737No Hit
AACTAG14450.10250706021810949No Hit
CCTCGG14150.10037888595752589No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)