FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27346554
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAT3796641.3883431162844138No Hit
AGCCTA3658121.3376895677605303No Hit
GGCGTT3407641.2460948461733057No Hit
CAGTAG3342411.2222417493626436No Hit
ATCCAC3298031.2060130135592222No Hit
AGGACT3035841.1101362167971875No Hit
ACTAGC3031781.1086515690423004No Hit
GTAAGA2874971.0513097920856866No Hit
GGTGGT2672640.9773224077885645No Hit
ACTTGA2564010.9375989384256606No Hit
CCTGCT2536530.9275501403211533No Hit
AATGTA2534340.9267493081578031No Hit
TACTCG2509580.9176951509137129No Hit
GAGCAC2503340.9154133277633446No Hit
AACAAT2489920.9105059452829047No Hit
CCGACT2466830.9020624682729678No Hit
CATTAA2453150.8970600098279293No Hit
TTACGT2441320.8927340534386892No Hit
GCTTCT2427720.8877608491366042No Hit
GGCTAC2407010.8801876828795321No Hit
GAATAC2398340.8770172651369529No Hit
AGTCAA2380130.870358290847176No Hit
AGTAGG2353880.8607592751905779No Hit
GACCGA2335520.8540454493827631No Hit
GGACTA2272820.8311175148430037No Hit
TTCGTT2186490.7995486378283713No Hit
TACGGC2179290.7969157649625617No Hit
GGCCAG2165980.7920486069286828No Hit
AAGTTG2162380.7907321704957779No Hit
CCTCGG2159580.7897082754924076No Hit
AGAACG2141150.7829688523095086No Hit
CAAGCT2128610.7783832654015567No Hit
GAGATA2098320.7673069155258099No Hit
CATGAG2082270.7614378030957758No Hit
GAGTCG2066430.7556454827909944No Hit
TAGCTT2034010.7437902413591124No Hit
GCCAAT1996860.7302053487251081No Hit
ACGTAA1995760.7298031042594983No Hit
GTTACA1979540.7238718267756881No Hit
GGATTC1957630.7158598483743144No Hit
CTCCGC1947850.7122835293982561No Hit
ATGCCG1941250.7098700626045973No Hit
CGAATT1895460.6931257225316213No Hit
AACTAG1874470.6854501667742122No Hit
CACATT1871850.684492093592487No Hit
TCCAGA1869790.6837387994114359No Hit
GGCCGT1862930.6812302566531784No Hit
GTACGC1849420.6762899632619159No Hit
CAGATC1842090.6736095524138069No Hit
CAACGG1829810.6691190414704536No Hit
CGTTGC1801780.6588691211331417No Hit
TCTTCA1798670.6577318663258267No Hit
GAGAAG1792360.655424445800374No Hit
TTAGTA1768490.646695740896641No Hit
GAAGAT1743720.6376379268846817No Hit
CGTACT1742240.637096725240043No Hit
TTCACA1737880.6355023744490804No Hit
GGAGGC1728170.6319516528481066No Hit
AGGCTT1714630.6270003891532366No Hit
AGTTAG1674160.6122014495866646No Hit
CTCGTC1673180.6118430863354849No Hit
TATGGT1669020.6103218709019059No Hit
TGCGAC1657520.6061165878523488No Hit
CCGCTC1626140.5946416502788615No Hit
TCTCGC1613150.5898915088167964No Hit
GGTAAT1606040.5872915468618093No Hit
AGACGG1595010.5832581319021036No Hit
AAGGCG1579430.5775608875619209No Hit
GATCAG1566130.5726973862959114No Hit
CGAGAG1544570.564813394769959No Hit
TGACCA1531380.5599901179505103No Hit
GACCAT1529100.5591563748763373No Hit
TCAATG1523660.5571670931555033No Hit
AGAGGT1508500.5516234330658262No Hit
AGGTGT1477490.5402837959034985No Hit
CAGCCT1434990.5247425324594829No Hit
AACATA1418800.5188222252792801No Hit
CCAGCG1410690.5158565865373751No Hit
TAAGTC1408210.5149497081058184No Hit
GTCGAG1406050.5141598462460755No Hit
GGTACC1398880.5115379436838733No Hit
TGATAA1394240.5098412033925738No Hit
CCATGA1388830.5078628919753472No Hit
CTTAGC1373100.5021107961171268No Hit
TCGTGG1368400.5003921152186122No Hit
GAGTGA1357060.4962453404549619No Hit
AAGCGC1336660.48878553400183444No Hit
ATGTGA1331300.48682550642395384No Hit
CTATGC1326780.4851726473470844No Hit
CACCTA1320750.4829676163219688No Hit
TGTGTG1301890.476070952120695No Hit
GCTCAA1300170.475441988047196No Hit
CTGGAG1294390.47332837621880985No Hit
ACGCTA1275520.4664280552496669No Hit
GCATAA1267850.4636233142940057No Hit
AGGTCA1263430.4620070228958282No Hit
ACAGTG1254260.45865376675978986No Hit
TTAGGC1251990.457823680453486No Hit
AGATGC1249770.45701187798652804No Hit
ACAGCC1245340.45539192982048127No Hit
CTTGTA1242400.45431684006694223No Hit
GATCCT1233730.4511464223243631No Hit
GCACAT1226670.4485647442087219No Hit
TTGAAT1226360.4484513844047773No Hit
CTGTAT1211920.44317101160168115No Hit
CGTGAA1206440.44116710280937044No Hit
TATAAG1197580.4379272064772768No Hit
AACCTT1194610.43684114642013033No Hit
ACCTGC1193690.4365047237761657No Hit
TATTCT1191940.4358647893990592No Hit
GCTGTC1190620.4353820960403274No Hit
GAGCCA1169750.4277504214973484No Hit
GGATCG1160450.4243496273790109No Hit
CATAAC1153840.4219325038174828No Hit
ATACCT1151020.420901295278374No Hit
TGCGGA1140480.4170470619442581No Hit
CCGGAA1127440.41227863664284725No Hit
ACCAGG1123250.41074645090566075No Hit
TCCTCC1118190.4088961263638556No Hit
CGTCAG1083670.39627296367944564No Hit
ATCAGC1078940.3945433124773235No Hit
ATTGAA1074150.3927917206679862No Hit
GTTGGC1067380.39031608882055124No Hit
GCGCAG1059010.3872553741140474No Hit
ATTCAT1053700.38531363037551275No Hit
ATGGCC1047190.3829330744926765No Hit
CGTCTC1016070.37155321288378784No Hit
TTCTGT991570.36259413160429643No Hit
CTATTA980020.35837056471539336No Hit
TCCACG977250.3573576400156305No Hit
TATTGG973950.35615090661880106No Hit
CAGTCC965200.3529512347332684No Hit
CTACCG952250.3482157203426801No Hit
CTAGTT948790.3469504786599438No Hit
GTCTGC947450.3464604717654736No Hit
CGAGCC936130.3423210105375617No Hit
GCGTAT930070.3401050092088385No Hit
AAGCAT913920.3341993291001126No Hit
GACACG900950.3294565011737859No Hit
TCAAGC893380.3266883278968165No Hit
ACACGA892660.32642504061023553No Hit
ATTGCT888170.3247831518369737No Hit
AATTGC885680.32387261663754785No Hit
ACTAAT868840.31771461954584845No Hit
CCACTT865730.31657736473853343No Hit
TGTGAT853340.3120466293486192No Hit
GCGGCT846420.30951614598314653No Hit
TCATGT844140.3086824029089735No Hit
CATACG843060.308287471979102No Hit
GACTAA821160.3002791503455975No Hit
ATTCGA816640.29862629126872803No Hit
CGAAGC812990.2972915709964773No Hit
CTGTTC798120.2918539571750064No Hit
CTGAGG797680.29169305938876244No Hit
TTCTAC790200.2889577970226157No Hit
GATTCA784180.2867564227653693No Hit
ATCCGG780040.28524252086752866No Hit
TAATGA773090.28270106719844845No Hit
TGGCAT771590.28215255201807143No Hit
ACGAAC668740.2445426944835536No Hit
TCTCTG666700.2437967138382408No Hit
NNNNNN660290.24145272563409634No Hit
TGCATA653040.23880156892894075No Hit
GTGGTG635450.23236931424705284No Hit
AGTGCA615330.22501189729426238No Hit
CCGTCG608050.2223497702854992No Hit
AAGAGG584720.2138185308467019No Hit
CGCTTG580980.21245089966362854No Hit
TTCCAT577660.21123685273106074No Hit
GCCTGG568560.20790919397010682No Hit
TAGAGC564750.20651596541194917No Hit
CAGGAC560390.20492161462098663No Hit
TCGGAC391930.14331970309677777No Hit
TACAGT360950.13199103623805764No Hit
GTACTT358890.13123774205700653No Hit
CAAGGA350730.1282538194757555No Hit
TGACAC323250.11820502137124846No Hit
GACTTG322390.11789053933449897No Hit
AACTCT320900.11734568092199112No Hit
CGACAA310900.11368891305281097No Hit
GGCAGG300280.10980542557574166No Hit
AGTTCC283730.1037534747522485No Hit
GTCTCA283470.1036583987876498No Hit
CTTCAC283020.10349384423353669No Hit
CCTGAC275700.10081709015329683No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)