FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27783234
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAAT5994702.1576681821849824No Hit
CTGTAT5771092.077184391133156No Hit
ATCAGC5081431.828955549235197No Hit
TGATAA4924971.772641010762102No Hit
GTCTCA4242791.52710444003747No Hit
CTAGTT4123501.4841684736917236No Hit
GACCAT3899561.4035659059704857No Hit
TATAAG3699381.3315152584468748No Hit
ATTCAT3533441.271788590197959No Hit
ATACCT3486751.2549834911227398No Hit
GTAAGA3463811.246726712952135No Hit
ATTGAA2937171.057173545743451No Hit
CCTGAC2828571.0180852236280342No Hit
TAGAGC2748630.9893124752863544No Hit
CTTCAC2740910.9865338210807281No Hit
GTCTGC2564970.9232078598193428No Hit
TTCTGT2564230.9229415121364202No Hit
GTACTT2486410.8949318139133839No Hit
GATCAG2473670.890346314615498No Hit
TTCCAT2460520.8856132443041007No Hit
ACTTGA2385540.8586257452966058No Hit
ACAGTG2336290.8408992272101944No Hit
TGACCA2331480.8391679672711967No Hit
GGCTAC2306580.8302057276701481No Hit
ATCCAC2244450.8078433201836762No Hit
CGAGAG2228200.8019944690384136No Hit
AGTGCA2225650.8010766493202339No Hit
TACAGT2211460.7959692525355399No Hit
TTAGGC2161200.7778792058548691No Hit
GAGAAG2155810.7759391869211483No Hit
ACCGCT2111940.7601490884754453No Hit
TATTCT2075910.7471808357515183No Hit
GAGCCA2067860.7442834048764805No Hit
AACTCT2039810.7341873879765041No Hit
TGACAC2018470.7265064966878946No Hit
TCGGAC2001290.7203229112924723No Hit
CAGTCC1959260.7051950827610638No Hit
GGCAGG1907300.6864931562682731No Hit
CAAGGA1885780.6787474777054392No Hit
GGATTC1873490.6743239465931145No Hit
TCAAGC1868220.6724271191755431No Hit
GCCAAT1839920.6622411199502549No Hit
AGTCAA1828250.6580407450047032No Hit
CGAGCC1813150.6526058125558745No Hit
CTATTA1811820.6521271065852161No Hit
TACGAA1805190.6497407753179489No Hit
GATCCT1787300.6433016401186413No Hit
GAGATA1760960.6338211023237972No Hit
TTCACA1737750.6254671432418558No Hit
AGTTAG1711700.6160909849443733No Hit
CAGATC1707240.614485700260812No Hit
CGACAA1691590.6088528066962975No Hit
GGACTA1669090.6007543974182415No Hit
GCCATA1645410.5922312715647142No Hit
TAAGTC1643170.5914250299299211No Hit
CAAGCT1631910.5873722259978806No Hit
AATCGG1619040.5827399358908325No Hit
GGTACC1614510.5811094561561839No Hit
TAGCTT1613660.5808035162501242No Hit
TATTGG1583750.5700380308498284No Hit
ACTAGC1580990.5690446259783868No Hit
GACTTG1578030.5679792352466959No Hit
CTATGC1558890.5610901884208297No Hit
CTTGTA1553090.5590025984735975No Hit
GCGCAG1537020.5532185346025592No Hit
GAACCG1531120.5510949517252024No Hit
GAAGAT1514080.5449617564319547No Hit
GGATCG1511760.5441267204530617No Hit
TCTTCA1482050.5334332209130154No Hit
ATTCGA1480750.5329653128213944No Hit
CTGTTC1478580.5321842662376886No Hit
ATGTGA1476920.5315867835976186No Hit
CCGTCG1474870.5308489285300624No Hit
CGTACT1433350.5159046639422898No Hit
CGAAGC1425290.5130036337742395No Hit
AACCTT1410560.5077018751668722No Hit
TAATGA1347720.485083917876515No Hit
CGAATT1340100.48234125660101335No Hit
AACTAG1315710.4735625809436007No Hit
CGTTGC1291420.46481989821631275No Hit
GGCCGT1276780.4595505332460577No Hit
GTACGC1263470.454759874246461No Hit
CCGGAA1261480.45404361493697964No Hit
CAGGAC1258520.45297822420528866No Hit
GACCGA1247390.4489722110824103No Hit
AGAACG1247010.44883543794793657No Hit
AAGCAT1239500.446132368895572No Hit
CGCTTG1232240.4435192821685193No Hit
AATGTA1227400.44177722435048417No Hit
CACATT1226920.4416044582858857No Hit
GCGTAT1225190.4409817805947285No Hit
ACCAGG1209390.43529489763502693No Hit
TACTCG1207730.434697414994957No Hit
GGAGGC1198280.4312960830981735No Hit
CTGGAG1191870.4289889362771807No Hit
GGCGTT1190090.42834826212096117No Hit
CTACCG1186940.4272144848220333No Hit
TCCTCC1180870.42502971396346445No Hit
TCTCGC1179290.4244610256674943No Hit
TCCACG1162450.418399816234496No Hit
AGGTCA1145210.41219463508099885No Hit
CATTAA1141360.4108089072711982No Hit
CTCGTC1125870.40523360239488315No Hit
CCTTAT1121480.4036535127624092No Hit
CCAGCG1113010.40060491158084766No Hit
CTCCGC1090850.39262887826521564No Hit
CCTGCT1086730.3911459695440783No Hit
GTGGTG1062590.38245727621197734No Hit
TGTGTG1058090.38083759435636616No Hit
AAGTTG1044400.3759101622222957No Hit
AGCCTA1035440.37268519568312314No Hit
ACACGA1024570.36877276417856897No Hit
TGCGAC1012750.36451839983783024No Hit
GGTAAT1011090.3639209171977604No Hit
TGTGAT1005080.3617577420972663No Hit
ACGAAC988700.3558621001428416No Hit
AACATA982640.3536809285772851No Hit
AACAAT965700.3475837262141621No Hit
CTGAGG959720.3454313489927055No Hit
TCATGT945140.34018357978052516No Hit
CGTCAG943360.3395429056243056No Hit
GTTACA941190.3387618590405998No Hit
AGGCTT923950.3325566778871027No Hit
GCACAT894630.3220035507745426No Hit
TGCGGA890860.3206466173088417No Hit
ACCTGC862830.3105577989948902No Hit
GTTGGC861760.31017267464255605No Hit
TATGGT860350.30966517432779783No Hit
CATACG859110.30921886199425164No Hit
ACGCTA857670.3087005638004561No Hit
TCTCTG856440.30825785075992235No Hit
TTCTAC852550.30685772577807174No Hit
GCTGTC850990.30629623606812656No Hit
CATAAC848310.30533162554078475No Hit
TGGCAT830020.2987485186209784No Hit
CAGTAG826560.297503163238664No Hit
CAGCCT820520.2953291902591325No Hit
AGGACT818530.29461293094965113No Hit
CGTCTC818140.29447255852216486No Hit
CACCTA798380.2873603555295255No Hit
CCGACT797370.28699682693526607No Hit
GACTAA795860.28645333369038317No Hit
TTGAAT787360.2833939346297843No Hit
CCATGA786360.28303400532853734No Hit
GAGCAC783660.2820621962151706No Hit
GCTTCT775730.27920795685628247No Hit
AGATGC767760.2763393203253444No Hit
ACGTAA765860.27565545465297525No Hit
GATTCA754700.2716386436510595No Hit
AAGGCG744910.268114935791852No Hit
GAGTCG744050.2678053965927797No Hit
GGCCAG743810.2677190135604804No Hit
ATTGCT728460.26219409878634No Hit
GACACG719610.25900872447030465No Hit
GGTGGT711770.25618687874852875No Hit
TTCGTT708880.2551466830679251No Hit
ATGGCC700250.252040493198164No Hit
GCTCAA691150.24876513655681695No Hit
TTAGTA690390.24849159028786932No Hit
TCAATG687480.24744419602124074No Hit
ACAGCC679440.24455036443921538No Hit
TCGTGG677390.2438125093716592No Hit
GCGGCT677240.24375851997647216No Hit
CCTCGG663260.23872670834504003No Hit
ATGCCG655970.2361028237389499No Hit
TACGGC653150.23508782310943355No Hit
GAGTGA620820.22345130880012024No Hit
AGACGG617960.22242191099855402No Hit
CATGAG599950.21593958428309679No Hit
TGCATA587090.21131089346906126No Hit
CAACGG581850.2094248639305273No Hit
CCACTT570980.20551243242597317No Hit
CGTGAA568960.20478537523745433No Hit
GCCTGG550470.19813028245739858No Hit
GTCGAG532640.19171274301616578No Hit
AGAGGT526140.18937320255806073No Hit
NNNNNN493970.17779427693694694No Hit
CTTAGC453080.1630767678089599No Hit
AGGTGT450060.16198978131919417No Hit
GAATAC445500.16034850370550816No Hit
AATTGC433830.15614812875995646No Hit
AAGAGG385580.138781539974792No Hit
CCGCTC346220.12461472267771274No Hit
TTACGT328820.11835195283601614No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)