FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001729955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19896174
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAC3746621.8830856626002566No Hit
GTCTCA3123761.5700304993311778No Hit
TACAGT2964491.4899799328252759No Hit
GTACTT2961161.4883062442055441No Hit
TAGAGC2895791.455450681120903No Hit
AGTTCC2780521.3975149192000431No Hit
TACTCG2605941.3097694059169365No Hit
AACTCT2550311.2818092563927115No Hit
GGCAGG2476301.24461114986228No Hit
AGCCTA2441671.2272057934354617No Hit
CCTTAT2292771.1523672842828978No Hit
GGCGTT2270011.1409278990020895No Hit
TAAGTC2227261.1194413559109404No Hit
GAATAC2160391.0858318790336272No Hit
ATGGCC2103611.0572937289350204No Hit
CCGCTC2061431.0360936730850865No Hit
CCGACT1977740.994030309545946No Hit
GGTAAT1935250.9726744448455267No Hit
GGCCGT1903310.9566211071535663No Hit
CTCCGC1902860.9563949330157648No Hit
CGAATT1863950.9368384092338558No Hit
TTAGCG1847580.9286106967098298No Hit
CGAGCC1839240.924418936022574No Hit
GTCGAG1758810.8839940784595068No Hit
GGCCAG1756500.8828330512187921No Hit
GAGCCA1743640.8763694969696183No Hit
TATTGG1735010.8720319796157793No Hit
GGATTC1681670.8452228051483667No Hit
GATCCT1678590.8436747688274137No Hit
GGTACC1640510.8245354106774498No Hit
TCCTCC1620670.8145636442463762No Hit
TGACAC1616790.8126135205693317No Hit
GACACG1537420.7727214287530859No Hit
CCTGAC1522740.7653431257688036No Hit
GAAGAT1521420.7646796816312523No Hit
CTATTA1503830.7558387858891865No Hit
AGTCAA1484640.7461937154349374No Hit
ACGAAC1474700.7411977800354983No Hit
AGGCTT1470190.7389310125655315No Hit
GCGTAT1453810.7306982739495543No Hit
CAAGGA1444580.7260591910786466No Hit
AGGTGT1440840.724179432688918No Hit
CTTAGC1422240.7148309016597865No Hit
TCTCGC1411140.7092519395940143No Hit
TCAAGC1407010.7071761636181911No Hit
ATGCCG1388420.6978326586810107No Hit
CAGTAG1372290.6897255723638123No Hit
ACACGA1365400.6862625950094726No Hit
CACCTA1361510.6843074452404769No Hit
TAGCTT1357720.6824025563909926No Hit
AAGCGC1323550.6652284001939267No Hit
GTTGGC1314030.6604435606564357No Hit
CTGGAG1298530.6526531181321594No Hit
ACCAGG1288910.647818017675157No Hit
TGCGGA1287720.6472199127329706No Hit
CATGAG1270570.6386001650367553No Hit
GTAAGA1259610.6330915682582993No Hit
CACATT1237830.6221447399887033No Hit
ACTTGA1196270.6012563018397407No Hit
CAGGAC1172410.5892640464443063No Hit
TCCACG1169100.587600410008477No Hit
CAAGCT1139700.5728236996721079No Hit
AAGTTG1133280.5695969486394721No Hit
ATCCGG1131890.5688983218582627No Hit
GGCTAC1127560.5667220240434167No Hit
CATTAA1114020.559916695541565No Hit
AGGACT1108470.557127214508679No Hit
ATGTGA1105350.5555590738199213No Hit
CAGATC1088380.5470297957788266No Hit
AACATA1078030.5418277906093906No Hit
TGGCAT1071980.5387870049789473No Hit
TCCAGA1069240.5374098557843332No Hit
GGACTA1066850.5362086198080094No Hit
CTTGTA1056780.5311473452132053No Hit
GCTGTC1051610.5285488556744629No Hit
AACCTT1050120.5277999679737421No Hit
GGATCG1037620.5215173530348096No Hit
CATACG1037220.5213163093567638No Hit
GAGATA1030670.5180242191287632No Hit
ACTAGC1022110.5137218844185821No Hit
CCATGA1002970.5041019444240888No Hit
GACTAA1000840.5030313868384947No Hit
ACAGTG986480.4958139187966491No Hit
GCGGCT982920.4940246300620411No Hit
ACGTAA980250.4926826635110851No Hit
AGGTCA978800.4919538801781689No Hit
CGTTGC974700.4898931824781991No Hit
TATTCT964410.48472133386046984No Hit
TCAATG957950.48147447846002955No Hit
TGCATA952280.4786246843237298No Hit
GATCAG952090.47852918857665805No Hit
TCGGAC948130.47653885616400427No Hit
ATCCAC945580.47525720271646194No Hit
CAGTCC943610.4742670626020862No Hit
GACCAT935510.470195928121658No Hit
CCTGCT922780.4637977130678491No Hit
GACTTG922450.46363185203346136No Hit
AAGCAT915540.46015882249521944No Hit
CTATGC907670.4562032881296675No Hit
ATTCGA872210.43838076607090387No Hit
AGTGCA871450.43799878308261675No Hit
TTCACA850540.4274892248127706No Hit
GAGCAC848110.42626788446864206No Hit
AATGTA847110.4257652752735275No Hit
CATAAC843290.4238453081481897No Hit
CGACAA832660.41850257240412153No Hit
ATCAGC813150.40869666700743573No Hit
CTGTTC809600.4069124043647789No Hit
CTGAGG805650.40492709804407623No Hit
GCCAAT787620.39586505425616No Hit
GAACCG780000.39203517218938677No Hit
TGACCA774590.3893160564438168No Hit
GCACAT764770.38438043414779144No Hit
TTCGTT749360.37663522645107544No Hit
CGTACT746870.3753837295552401No Hit
ATTGCT732550.3681863658811991No Hit
TGATAA730730.36727161714609047No Hit
AATTGC694320.348971616351968No Hit
TTCCAT690880.34724264072077377No Hit
TCATGT679750.34164860037914824No Hit
GCCATA673830.3386731539440698No Hit
CCAGCG662090.33277252199342444No Hit
GTGGTG659400.3314205032585662No Hit
ATTGAA659110.33127474659198297No Hit
CCGTCG657870.3306515111900409No Hit
TTAGGC649660.32652508969815No Hit
GATTCA641060.32220265062016445No Hit
GCCTGG618580.3109039959139883No Hit
TGCGAC614260.3087327241910932No Hit
TCTTCA606540.3048525812048085No Hit
TTCTAC594360.29873080120831275No Hit
GCGCAG585130.294091718337405No Hit
ACCTGC583060.29305131730351774No Hit
GAGTCG580040.29153343753427163No Hit
CCGGAA573910.28845244316821916No Hit
CCACTT573240.2881156950074924No Hit
GAGTGA571460.2872210506401884No Hit
TACGAA566670.28481355259558944No Hit
GCTCAA566040.2844969088026673No Hit
TTAGTA560370.2816471146663675No Hit
AGAACG551960.2774201713354537No Hit
TGTGAT550560.2767165184622933No Hit
CTAGTT549550.27620888317522757No Hit
AGATGC546230.2745402206474471No Hit
CCTCGG544140.2734897674296576No Hit
AACTAG543800.27331888030331863No Hit
CTACCG532580.2676796051341328No Hit
AAGAGG522270.2624977043325013No Hit
AGTAGG514560.25862258743816774No Hit
TACGGC490060.24630866215786007No Hit
GGAGGC489350.24595180962932875No Hit
CTCGTC489000.2457758964110386No Hit
TAATGA484510.24351918112497406No Hit
GTACGC483660.24309196330912666No Hit
ACAGCC482190.24235312779230822No Hit
ACTAAT480780.2416444488271966No Hit
AGTTAG471000.23672893089897584No Hit
CAACGG462240.23232607434977198No Hit
ATTCAT457140.22976276745468754No Hit
CTGTAT450490.22642041630717546No Hit
TCGTGG443090.2227011082633274No Hit
CGCTTG437670.2199769664258063No Hit
CGAAGC437280.21978094883971158No Hit
GAGAAG423870.2130409595332248No Hit
ATACCT422050.21212621079811628No Hit
TTACGT416720.20944730378815546No Hit
CGTGAA402080.20208910517167775No Hit
GTCTGC367620.18476919230802868No Hit
TTCTGT354150.17799904644983502No Hit
TTGAAT314130.15788462646134882No Hit
TATAAG296880.14921461784562198No Hit
ACGCTA279620.140539583137944No Hit
AAGGCG224360.1127653990159113No Hit
CAGCCT222970.11206677223470202No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)