FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23471010
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCT4241821.8072592530104157No Hit
TCGTGG3753641.5992664993964896No Hit
GACCGA3410771.4531841620790924No Hit
CCATGA3278741.396931789471352No Hit
ACCTGC3245351.3827057293231095No Hit
CATTAA3202241.3643383902098802No Hit
AACTAG3131731.3342970754134569No Hit
TATGGT3076051.3105741934411856No Hit
CCTCGG3048221.2987170130301167No Hit
AGTAGG2973341.266813826929476No Hit
ACAGCC2966371.2638442061078754No Hit
AGGCTT2956831.2597796174940918No Hit
ACTAGC2894881.2333853549548996No Hit
GTTACA2863731.2201136636216336No Hit
CGTTGC2859761.2184222153200908No Hit
AGGACT2858381.2178342559608641No Hit
AGCCTA2800391.1931271811481483No Hit
ACGTAA2782781.1856243084554094No Hit
TACGGC2764551.1778572801085252No Hit
GAGTCG2540691.082480046661818No Hit
CCTTAT2448371.0431464176445753No Hit
CACCTA2441121.0400575007211024No Hit
GCATAA2408571.026189328878476No Hit
GCACAT2400271.022653051572983No Hit
CAACGG2354861.0033057801943759No Hit
AACAAT2298110.9791270166899507No Hit
AATGTA2290400.9758421133134024No Hit
CAGTAG2242110.9552677963155398No Hit
AGATGC2230660.9503894378639863No Hit
GCTTCT2227660.9491112653439284No Hit
AAGTTG2192160.9339862238565788No Hit
GGCGTT2141640.9124617986188068No Hit
GAATAC2140840.912120952613458No Hit
GGCCAG2132650.9086315416337004No Hit
TCAATG2122900.9044774809435129No Hit
GAGTGA2116690.9018316638269933No Hit
GAGCAC2079870.8861442264308181No Hit
TGTGTG2060780.8780107886281844No Hit
TTAGTA2058480.8770308563628068No Hit
AAGAGG2019360.8603634867012541No Hit
TTCGTT1995860.8503511352941352No Hit
AACCTT1986290.8462737649551512No Hit
CCGACT1858110.7916617137481515No Hit
TAGCTT1837280.782786935883884No Hit
AAGCAT1805550.7692681311967401No Hit
GTAAGA1777260.7572149643325958No Hit
ATGCCG1751160.7460948634080936No Hit
ATGGCC1726530.7356010670184198No Hit
ACTTGA1718640.732239473290668No Hit
GGTGGT1717560.7317793311834472No Hit
TAAGTC1673970.7132074844670084No Hit
GAGATA1671070.7119719176976194No Hit
GATCAG1644290.7005620976685707No Hit
ACCGCT1632480.6955303585146101No Hit
AGACGG1605270.6839373337576866No Hit
CTTGTA1572380.6699243023627871No Hit
CATACG1525120.6497888245968112No Hit
TCAAGC1517610.6465891327216No Hit
ATCCGG1478810.6300581014621868No Hit
CTGTTC1442850.6147370735217615No Hit
AGTCAA1437620.6125087927617943No Hit
TATTCT1436830.6121722073315123No Hit
CGAATT1418870.6045202145114335No Hit
ATCCAC1389420.5919728209395335No Hit
ACGAAC1365990.5819902935578827No Hit
GGACTA1354600.5771374985567302No Hit
TTACGT1319070.5619996753441799No Hit
GTACTT1313320.5595498446807359No Hit
TAATGA1302730.5550378956849322No Hit
TACTCG1275450.5434150469025406No Hit
TGCATA1266610.5396486985434372No Hit
GGCTAC1238410.5276338768548946No Hit
CTTCAC1227550.5230068923322857No Hit
TACAGT1159610.4940605453280451No Hit
TCTCGC1159590.49405202417791133No Hit
TGACCA1114740.4749433450030484No Hit
GAGAAG1110530.47314964289990075No Hit
AATCGG1105220.47088727753939863No Hit
TAGAGC1101350.46923843498852413No Hit
CTCGTC1101220.46918304751265494No Hit
GGCCGT1089130.4640320122568224No Hit
GCCAAT1077230.45896192792726004No Hit
GTCTCA1068890.45540860832149965No Hit
CACATT1065220.4538449772719623No Hit
CCGCTC1048620.4467724226609762No Hit
AACTCT1025720.43701570575786897No Hit
CGTCAG1023280.4359761254415553No Hit
ACGCTA1020990.43500045375124463No Hit
ACAGTG1017790.4336370697298498No Hit
TTAGGC1015880.4328232998920796No Hit
GTTGGC996310.4244853544862364No Hit
CAGATC989750.4216904172423768No Hit
AGTTCC984810.4195856931593485No Hit
GGATTC981030.41797519578407577No Hit
CAGCCT978620.41694839719296267No Hit
TGACAC977830.41661181176268086No Hit
GGAGGC972410.4143025800764432No Hit
CTGGAG959820.40893851606726767No Hit
GGTAAT931160.39672770792564954No Hit
TCCAGA923050.3932723815464268No Hit
GTACGC921030.39241174538292134No Hit
AGGTGT878000.3740784908702267No Hit
CTTAGC877280.37377172946541287No Hit
CTCCGC868750.37013745893338207No Hit
CGTGAA859160.36605156744426426No Hit
TTGAAT844280.35971183174477794No Hit
ACCAGG841300.3584421803748539No Hit
AAGCGC794480.33849416791181974No Hit
GCGTAT785130.3345105302243065No Hit
AAGGCG778270.33158777572844117No Hit
AACATA767770.3271141719082391No Hit
CAAGGA754480.32145186764438344No Hit
GTCGAG750490.31975189819270666No Hit
TCCTCC743410.3167354110453704No Hit
TTAGCG741650.3159855498336032No Hit
GATCCT730630.31129039610992454No Hit
GGCAGG729530.31082173285257003No Hit
GGTACC728120.3102209917681429No Hit
CCTGAC697080.29699616676061236No Hit
CGAGAG697030.29697486388527805No Hit
CGAGCC671530.2861103974647874No Hit
GCTGTC669620.2852966276270173No Hit
CTATTA651330.2775040358297321No Hit
GACACG650610.27719727442491826No Hit
TCTCTG645890.27518628299336073No Hit
TATTGG636370.2711302155297109No Hit
GAAGAT620460.26435164059833816No Hit
TCTTCA612900.26113064584779266No Hit
CAGGAC595150.2535681251041178No Hit
TGCGGA581950.24794416601586383No Hit
CATGAG581090.24757775656011394No Hit
TCCACG557570.2375568840028614No Hit
TGCGAC552110.23523061001635634No Hit
GAGCCA526250.2242127628934588No Hit
ACACGA517400.22044215395928848No Hit
AATTGC491640.20946691258705952No Hit
GCGGCT477310.20336150851625046No Hit
TTCTAC472110.20114600948148376No Hit
AGAACG467670.19925431415179834No Hit
CGTACT446550.19025597961059196No Hit
ACTAAT440170.1875377327179359No Hit
GACTAA429700.1830769106229344No Hit
CTACCG427670.182212013884362No Hit
CCAGCG422670.1800817263509325No Hit
TTCACA422450.17998799369946158No Hit
CCGGAA419420.1786970394542033No Hit
ATACCT416790.17757650821161935No Hit
ATGTGA404400.17229765570378094No Hit
GGATCG401940.17124955423733362No Hit
TGATAA392410.16718922619861692No Hit
GCTCAA382710.16305646838376364No Hit
GCGCAG382470.162954214582159No Hit
AGGTCA377630.16089209624979922No Hit
GTGGTG366960.1563460626534606No Hit
ATTCGA366610.1561969425261205No Hit
ATCAGC348050.14828931520203006No Hit
CTGAGG330860.14096538666209935No Hit
TCATGT324770.13837069644638214No Hit
CCACTT318490.13569505530439466No Hit
AGTGCA285860.1217927988612335No Hit
ATTGCT281140.11978180742967602No Hit
GATTCA279830.11922367209591747No Hit
ATTGAA278740.11875926941362983No Hit
CCGTCG272930.1162838752997847No Hit
CTGTAT268220.11427714444329409No Hit
CGAAGC266020.11333981792858511No Hit
TGTGAT260850.11113710061901895No Hit
GACCAT260510.11099224106674574No Hit
ATTCAT259140.11040854228258606No Hit
CTAGTT253010.10779680976660143No Hit
CGCTTG249750.10640786229480538No Hit
CAGTCC242180.10318260696919307No Hit
GCCATA240470.10245404863276017No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)