FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25515295
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGC5260742.061798619220354No Hit
GAGTCG5082111.9917896304941802No Hit
GTTACA5041831.9760030209331303No Hit
TAATGA3658451.4338262598962699No Hit
GAGATA3470211.3600509028016332No Hit
ATCCGG3449531.3519459602563872No Hit
TATGGT3280681.285769966602385No Hit
CAACGG3173361.2437089204729948No Hit
CCTCGG3156801.2372186956882136No Hit
AACCTT3099781.2148713154208095No Hit
GAGTGA2901691.1372355287289448No Hit
ACGAAC2884231.1303925743362953No Hit
GGACTA2819181.1048980621231304No Hit
CTGTTC2729121.0696015860290857No Hit
ACGTAA2726861.068715842791549No Hit
TAAGTC2645371.036778136407986No Hit
TCATGT2633971.032310228041651No Hit
AAGCAT2615821.0251968476163023No Hit
GTAAGA2592311.0159827664152032No Hit
CATACG2568501.0066511086781478No Hit
TACGGC2404650.9424347239567483No Hit
AGGTGT2400590.9408435215034747No Hit
ACTAGC2362200.925797644118949No Hit
ATCCAC2348340.9203656081577736No Hit
CTATGC2334140.9148003187891811No Hit
TGCATA2323660.9106929784664453No Hit
ACAGCC2269020.8892783720509599No Hit
CGTTGC2202700.8632861191689143No Hit
AACTAG2178370.8537506621028682No Hit
TCCTCC2138130.8379797294132794No Hit
GAGCAC2121780.8315718082036676No Hit
GCTTCT2110040.8269706464299158No Hit
CACATT2028480.7950055055212962No Hit
AATGTA2022960.7928420972597024No Hit
AGGACT2016640.7903651515688923No Hit
TATTCT1985930.7783292335048448No Hit
ACCGCT1967120.7709571847003924No Hit
GACCGA1963610.7695815392297052No Hit
CATTAA1948350.7636008127674009No Hit
AAGTTG1939850.7602694775819758No Hit
CGAGCC1932590.7574241254118363No Hit
ACACGA1886550.7393800463604282No Hit
GGTACC1837790.7202699400496839No Hit
TGGCAT1783060.6988200606734117No Hit
GAAGAT1779050.6972484543094641No Hit
CCGCTC1771790.6944031021393247No Hit
CGAATT1749450.6856475694284545No Hit
GGTAAT1727550.6770644823036536No Hit
AACATA1649110.6463221373689781No Hit
GATTCA1632980.6400004389524009No Hit
TCAATG1599460.6268632206682305No Hit
CTGGAG1592350.6240766567660692No Hit
TCCACG1591500.6237435232475266No Hit
GTGGTG1578020.6184604175652291No Hit
ATGGCC1574130.616935841815664No Hit
TACTCG1571850.6160422601423969No Hit
TGTGAT1538720.6030578913549696No Hit
GCGTAT1534170.601274647226301No Hit
CAGGAC1528090.5988917627642557No Hit
ATTCGA1524370.5974338137183991No Hit
GGCCGT1507110.5906692436830536No Hit
GGATTC1475730.5783707380220373No Hit
TTCTAC1436090.562834958404361No Hit
TTCGTT1433490.5618159617594074No Hit
GACACG1430510.560648034835576No Hit
CTTAGC1427780.5595780883583749No Hit
AGTCAA1417230.5554433135105826No Hit
TCCAGA1403130.5499172163206422No Hit
AGCCTA1393930.5463115358846528No Hit
CCACTT1375080.5389238102087395No Hit
GATCCT1362590.5340287070950972No Hit
TATTGG1334200.5229020475757776No Hit
ATTGCT1315140.515432018324695No Hit
GAGCCA1314810.5153026841351432No Hit
GTTGGC1298690.5089849049364312No Hit
TTCACA1289250.5052851632716768No Hit
CATGAG1282170.502510357023111No Hit
GCGGCT1275470.49988448105342304No Hit
AAGCGC1262100.49464448676764267No Hit
TGCGGA1255020.4918696805190769No Hit
AATCGG1254040.4914855971682867No Hit
ACCAGG1253000.49107799851030526No Hit
GCTGTC1238230.48528931372339607No Hit
GGCGTT1230190.48213826255977055No Hit
ACTAAT1214390.4759458983327451No Hit
GCCTGG1194820.46827598897053707No Hit
CCTTAT1192340.467304022939966No Hit
TCTCGC1180320.46259312306598843No Hit
AATTGC1170390.45870133972583893No Hit
CTGAGG1166700.45725514833357794No Hit
CTCCGC1161060.4550447094576018No Hit
CCGACT1083880.42479618597394225No Hit
AAGGCG1065600.41763185571634587No Hit
GGCCAG1053550.41290919818877264No Hit
GTCGAG1047200.4104204948443668No Hit
AGATGC1044320.40929176009918755No Hit
CAGTAG1037810.4067403492689385No Hit
CTTCAC1014390.3975615410286262No Hit
TGATAA1006430.3944418436079222No Hit
TGTGTG993480.3893664564724805No Hit
TGCGAC983130.3853100659819924No Hit
ATGCCG967840.3793175818660925No Hit
GCGCAG950520.37252949652355577No Hit
ACTTGA949490.37212581708343956No Hit
GACTAA916560.35921983265331636No Hit
AACAAT907130.3555240102064272No Hit
CGTACT902230.3536035934524763No Hit
GCTCAA897260.3516557421734689No Hit
GATCAG896390.351314770219196No Hit
TAGCTT893460.3501664393846906No Hit
TCTTCA879200.34457763470890695No Hit
CCGGAA864400.33877719226840214No Hit
AGTTCC849760.33303945731374063No Hit
GGTGGT834600.3270979230300884No Hit
CCTGCT833960.3268470930867152No Hit
GCCAAT829390.32505601052231614No Hit
GAATAC820300.3214934414828439No Hit
GGATCG802810.3146387294365987No Hit
AGAACG800730.3138235321206359No Hit
GGCTAC795610.3118168925736505No Hit
AGTGCA795070.3116052548089293No Hit
CAGCCT791990.31039813570644587No Hit
CCAGCG781530.30629863381944045No Hit
ATTGAA779760.305604932257299No Hit
GTCTCA772660.3028222875730028No Hit
TGACAC736030.2884661925327534No Hit
AGGTCA735560.28828198929308874No Hit
TTAGTA733120.28732570013397846No Hit
CTAGTT717580.2812352355714484No Hit
CCATGA716470.2808002023884106No Hit
CAAGCT702340.27526234754487455No Hit
GACTTG695380.2725345719106912No Hit
CAGATC694650.27224846900653116No Hit
CGCTTG690140.27048090174932327No Hit
TACAGT685560.26868589996705894No Hit
TCGTGG681600.26713388969243745No Hit
CTTGTA678650.2659777204222017No Hit
CAAGGA678150.26578175952894134No Hit
ATCAGC676330.2650684618774739No Hit
ACCTGC674620.26439827562252366No Hit
GACCAT674060.2641787994220721No Hit
CCTGAC673970.26414352646128525No Hit
ACAGTG669190.2622701403217168No Hit
GCCATA664270.2603418851320355No Hit
AGGCTT652560.25575248101187936No Hit
TTCCAT646000.25318147409230424No Hit
GTACTT639100.2504772137653121No Hit
ATGTGA628290.24624053925302453No Hit
NNNNNN613460.2404283391589241No Hit
ATACCT599100.23480034230448835No Hit
CACCTA599010.2347650693437015No Hit
CTCGTC598390.23452207783605872No Hit
CGTGAA591010.23162969505153672No Hit
AGTTAG587370.23020309974860179No Hit
GTCTGC586060.22968968220825978No Hit
GGCAGG585250.22937222556117812No Hit
TAGAGC579650.2271774635566628No Hit
GAGAAG576990.226134951604518No Hit
ATTCAT550570.21578037800464386No Hit
CGTCAG547200.21445960158406946No Hit
CTGTAT537930.21082648662302356No Hit
CGAAGC533780.20920001120896312No Hit
GGAGGC532180.20857293635053015No Hit
TCGGAC527240.2066368427251184No Hit
CAGTCC503620.19737965012750197No Hit
GTACGC498350.19531422231253842No Hit
TGACCA478770.18764039373246516No Hit
TTACGT461540.18088758135071534No Hit
CCGTCG453370.17768558035484205No Hit
ACGCTA435950.1708583028336533No Hit
TACGAA415930.163012028667511No Hit
TTCTGT407300.15962974364983826No Hit
CATAAC406650.15937499448859987No Hit
TTAGGC405230.15881846555174062No Hit
CTACCG393340.15415851551001075No Hit
CGACAA390070.1528769312680884No Hit
AACTCT389490.15264961663190646No Hit
AGACGG384420.15066257317424706No Hit
TTGAAT348810.13670623835624868No Hit
AAGAGG316070.1238747190655644No Hit
TTAGCG309410.12126451996733723No Hit
GAACCG255940.10030846204208105No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)