FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24372680
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCC5771802.3681433473873206No Hit
GAAGAT5717942.34604483380572No Hit
GATCCT5526392.267452738065736No Hit
ACACGA5350812.1954130608533817No Hit
GGTACC5257962.1573171272096463No Hit
GAGCCA5183262.1266680562006313No Hit
CGAGAG4873371.999521595491345No Hit
TCCACG4423591.8149789026073455No Hit
TATTGG4250651.7440224054145872No Hit
GGATCG4207261.7262196853197926No Hit
CTATTA4025481.6516361762432363No Hit
CAGGAC3694831.5159719817434931No Hit
CCTGCT3193831.310413955297489No Hit
GCTGTC3192501.3098682623330713No Hit
CCGCTC2720121.1160528920085933No Hit
GCGTAT2571741.0551732513617706No Hit
TCCTCC2431060.9974528857721022No Hit
AACATA2394080.9822801595885229No Hit
TGCGGA2357940.9674520815929967No Hit
GACACG2344610.9619828430849624No Hit
GAATAC2309790.947696355099234No Hit
GTTGGC2305600.9459772171135878No Hit
GGCGTT2254460.9249947071885405No Hit
AGCCTA2229100.9145896142730302No Hit
ACCAGG2216910.9095881125916395No Hit
ACTAGC1982230.8132999735769723No Hit
GACCGA1947780.7991652949121723No Hit
CCTTAT1947050.7988657792249354No Hit
TCGTGG1930190.7919481977361538No Hit
CAAGGA1923260.789104850184715No Hit
AATGTA1903800.7811205004948163No Hit
CTTCAC1902010.7803860716178935No Hit
CCGACT1837270.7538235434100805No Hit
AACAAT1798600.7379574178957751No Hit
CATTAA1795940.73686603196694No Hit
TACTCG1769200.7258947313139138No Hit
GTACTT1740470.714106942691571No Hit
GCGGCT1736030.7122852308404328No Hit
GGCCAG1729800.7097290901123717No Hit
ACTTGA1644010.6745298424301308No Hit
AAGTTG1619420.6644406770203359No Hit
CGTTGC1603870.6580605825867324No Hit
GTCTCA1581700.6489643321949002No Hit
CAGTAG1562080.6409143352310867No Hit
GAGCAC1560430.6402373477188393No Hit
TCTCGC1534660.6296640336639221No Hit
AGGACT1527450.6267058033831323No Hit
CATGAG1485410.6094569821619945No Hit
CTTAGC1464190.600750512459032No Hit
AGTTCC1462080.5998847890342793No Hit
TAGAGC1446820.5936236802846465No Hit
GACTAA1430240.5868209815252159No Hit
AGGCTT1415140.5806255200494981No Hit
ATGCCG1374730.5640454804313683No Hit
ACCTGC1363820.5595691569412965No Hit
TCAATG1332070.5465422760238102No Hit
CCATGA1330440.5458734944208024No Hit
ATGGCC1314690.5394113408948051No Hit
TACAGT1304040.5350416942248453No Hit
GCTTCT1297860.5325060682698824No Hit
AGGTGT1289430.5290472775254916No Hit
GGTGGT1289070.5288995711591832No Hit
GGCAGG1282400.5261629004278562No Hit
GGCTAC1282120.5260480176985051No Hit
CTTGTA1280440.5253587213223987No Hit
CAGATC1274050.5227369333204227No Hit
CAGCCT1270200.5211572957918457No Hit
CTCGTC1253440.5142807438492607No Hit
GGTAAT1250530.5130867840549337No Hit
CACCTA1212910.4976514687756947No Hit
AGAGGT1209780.49636724397973464No Hit
GCACAT1195830.4906436222852801No Hit
TTCGTT1194980.4902948711426072No Hit
CTCCGC1173040.4812929887070277No Hit
TGACAC1164490.4777849625072007No Hit
AGATGC1159620.4757868236074162No Hit
AACTCT1148430.4711956173879934No Hit
GATCAG1137330.4666413377601478No Hit
AAGCAT1093330.44858833743355264No Hit
GGCCGT1090330.4473574510476484No Hit
GCCAAT1090180.44729590672835323No Hit
TGTGTG1076610.44172819730944646No Hit
AGACGG1046940.42955473095285374No Hit
TTAGTA1041570.4273514443220852No Hit
TCCAGA1030820.42294076810592846No Hit
CCTGAC1029420.4223663544591731No Hit
GAGTCG1018540.4179023398329605No Hit
TAGCTT1016720.4171556020921786No Hit
GTCGAG1014150.4161011427549207No Hit
CGCTTG1012390.41537902274185684No Hit
AAGAGG979690.40196236113550093No Hit
GACCAT979570.4019131256800647No Hit
AAGCGC966050.3963659310342564No Hit
TGACCA919670.3773364275081772No Hit
ATTCGA909670.37323347288849645No Hit
GAGAAG892970.36638153867362966No Hit
TCTTCA880250.36116258039739574No Hit
TTCTAC852770.3498876611025131No Hit
ACGAAC850930.3491327174524919No Hit
TTACGT844700.3465765767244308No Hit
GTTACA842530.34568623557196004No Hit
CTGTTC833230.341870487775657No Hit
ACAGTG825900.3388630220394311No Hit
ACGTAA825100.33853478566985656No Hit
AACCTT809550.332154691236253No Hit
CGTCTC809230.33202339668842323No Hit
GATTCA801470.32883950390355104No Hit
AGTGCA795010.3261889952192373No Hit
ACTAAT793320.32549559588851124No Hit
TAAGTC790560.32436318041347934No Hit
ATCCGG772360.31689580300566045No Hit
TATGGT768020.315115120700719No Hit
TTAGCG761280.3123497292870542No Hit
GGAGGC756330.3103187667503122No Hit
ATTGCT744540.30548138325370866No Hit
TCATGT735170.3016369147750678No Hit
AATTGC725480.2976611517485972No Hit
AGTAGG713920.2929181362082463No Hit
GTACGC712590.2923724432438287No Hit
TGTGAT698450.2865708654116002No Hit
GGATTC691370.28366597354086626No Hit
TTAGGC671500.27551340271156066No Hit
AACTAG658090.27001134056656884No Hit
ATCAGC655160.2688091748630024No Hit
CCTCGG652290.267631626887154No Hit
GTGGTG645380.2647964852449546No Hit
TCAAGC636520.2611612674519175No Hit
TACGGC626990.25725115169936175No Hit
CACATT619040.2539893027767156No Hit
TGCGAC617230.25324666799055334No Hit
AAGGCG612800.2514290590940348No Hit
TTCACA611420.2508628513565188No Hit
CAGTCC607620.2493037286010402No Hit
GAGTGA601350.24673117605450035No Hit
TCGGAC596330.24467149283542064No Hit
TTCCAT583790.23952638774234103No Hit
ACGCTA581110.23842679590426658No Hit
TACGAA570200.23395047241419492No Hit
GACTTG565210.23190309805897424No Hit
CGACAA563610.23124662531982532No Hit
CTGAGG555500.22791912912326426No Hit
TAATGA553020.22690159637758345No Hit
GCCTGG545290.2237300124565702No Hit
CGTACT541830.22231039015816068No Hit
AGGTCA524770.21531074957698537No Hit
ACAGCC517130.2121760922475493No Hit
CGTGAA512540.21029283607711585No Hit
TGCATA497210.20400300664514529No Hit
TTGAAT494760.2029977827633235No Hit
CCACTT478120.19617046627617482No Hit
CAACGG477990.19611712786611896No Hit
CGTCAG473250.19417232737639029No Hit
CGAAGC469780.19274860212336106No Hit
GCCATA465080.19082021345211114No Hit
CTACCG454500.18647928746448894No Hit
CCGTCG453820.18620028655035062No Hit
GTAAGA453480.18606078609328147No Hit
CGAATT450740.184936576527489No Hit
ATCCAC450320.1847642524334624No Hit
AGTCAA439050.1801402225770822No Hit
ACCGCT438950.1800991930308854No Hit
TGATAA438600.17995558961919655No Hit
GCATAA432710.17753894934820463No Hit
GCGCAG422920.1735221567755372No Hit
TATTCT416490.1708839569550825No Hit
ATGTGA414560.17009208671348414No Hit
AGAACG401080.1645613038861545No Hit
AGTTAG387390.1589443590118116No Hit
GAGATA387290.15890332946561478No Hit
CATACG385860.15831660695500044No Hit
CCAGCG372290.1527488975360937No Hit
GGACTA369320.1515303200140485No Hit
TCTCTG357690.14675858379135984No Hit
GAACCG354360.14539229990300614No Hit
ATACCT332290.13633707905737077No Hit
ATTGAA331610.1360580781432325No Hit
CCGGAA322680.13239413966785762No Hit
GCTCAA320080.13132737146674062No Hit
CTAGTT316650.12992005803219014No Hit
AATCGG292080.1198390985316346No Hit
CTGTAT287200.11783685667723041No Hit
CTGGAG287040.11777120940331552No Hit
TTCTGT275710.11312256181921726No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)