FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21697464
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC3723831.7162512632812754No Hit
GAGCCA3678891.6955391653144347No Hit
ACACGA3568321.6445792927689613No Hit
GATCCT3539551.6313196786500026No Hit
CGAGCC3426661.5792905567212832No Hit
GAAGAT3310281.5256529518841464No Hit
TCCACG3103971.4305681069455858No Hit
CGAGAG2952311.3606705373494339No Hit
TATTGG2820991.3001473351908777No Hit
AATGTA2783691.2829563860550708No Hit
CAGGAC2684891.2374211105961508No Hit
AACATA2506371.1551442140887986No Hit
CTATTA2437121.1232280417656182No Hit
GACACG2329931.073825954959529No Hit
GACCGA2297281.0587781134237624No Hit
CCGCTC2291421.0560773369643568No Hit
CATTAA2157720.9944572324212636No Hit
TCCAGA2066500.9524154527920867No Hit
GGATCG2054450.946861808366176No Hit
GGCGTT2034390.9376164882679376No Hit
TGCGGA1965100.9056818806105635No Hit
CATGAG1944210.8960540273278019No Hit
AAGTTG1933550.8911410107651291No Hit
CCTTAT1932250.8905418624038274No Hit
GCGTAT1909670.880135116251374No Hit
ACTAGC1885250.8688803447260012No Hit
CTCCGC1865380.8597225924651839No Hit
TACTCG1858420.8565148443154462No Hit
AGCCTA1827220.8421352836442084No Hit
GACTAA1816410.8371531345783082No Hit
AGGACT1795750.8276312844671616No Hit
CAGTAG1739570.8017388575918365No Hit
AACAAT1730420.7975217748949831No Hit
GAGCAC1720780.7930788593542545No Hit
CCATGA1716350.7910371460922806No Hit
CGTTGC1696020.7816673874882336No Hit
ACCAGG1691130.77941366788303No Hit
GCGGCT1631840.7520878937741295No Hit
TAGAGC1616420.7449810724423831No Hit
TCCTCC1594380.7348232033015472No Hit
GCACAT1591650.7335649917428139No Hit
TCAATG1573340.7251262175155585No Hit
CCTGCT1500200.691417208942022No Hit
GATCAG1486990.6853289398244883No Hit
CCGACT1477810.6810980306269895No Hit
GCTTCT1451630.6690321043970853No Hit
GCTGTC1441260.664252744007318No Hit
AGTTCC1421580.6551825595839219No Hit
CACCTA1415010.6521545559425747No Hit
GGCCAG1412720.6510991330599741No Hit
AGGCTT1403450.6468267443605391No Hit
CTTCAC1400860.6456330564714844No Hit
GTTGGC1389880.6405725572352603No Hit
TACGGC1388170.6397844466984712No Hit
TTCGTT1339480.6173440361509529No Hit
GGTGGT1336290.6158738182489899No Hit
TACAGT1329760.6128642499418365No Hit
GTACTT1329540.6127628556037701No Hit
TGTGTG1313470.6053564600913729No Hit
ACTTGA1298370.5983971214331777No Hit
GGTAAT1298100.5982726829273689No Hit
GAATAC1290510.5947745782640773No Hit
TTAGTA1265390.5831971883903114No Hit
CCTGAC1259960.5806945917734901No Hit
ATGGCC1253270.5776112821295613No Hit
AGGTGT1243150.572947142578506No Hit
ATGCCG1230120.5669418324648448No Hit
CCTCGG1217510.5611300933602195No Hit
CAGCCT1212780.5589501150917914No Hit
AGAGGT1190670.548759984116116No Hit
GGCTAC1163750.5363530042036249No Hit
AAGCGC1157070.5332743033932444No Hit
CTTAGC1125900.518908569222652No Hit
TGACAC1116620.5146315716896684No Hit
TCTCGC1109200.511211817196701No Hit
CAGATC1107240.5103084858212001No Hit
CAAGGA1107090.510239353317973No Hit
GGCCGT1088840.5018282320920086No Hit
ACAGTG1086520.5007589827087626No Hit
TAGCTT1075880.4958551838131866No Hit
GTCTCA1073410.49471680192671363No Hit
TCAAGC1057410.48734266824915573No Hit
GTCGAG1051580.4846557182903956No Hit
TCGTGG1044280.4812912698000098No Hit
AACTCT1023620.4717694196888632No Hit
GCCAAT1007240.46422015033646336No Hit
TATGGT978400.4509282743826652No Hit
AGATGC975090.4494027504781204No Hit
ACGTAA961210.44300568951283886No Hit
GAGAAG956850.4409962380857044No Hit
GGAGGC956330.44075657874118374No Hit
CATACG952400.4389453071566336No Hit
GTACGC927280.4273679172828677No Hit
TAAGTC908820.4188600105523853No Hit
GGCAGG907890.41843138903237725No Hit
TGACCA903290.4163113256000794No Hit
AAGCAT891930.4110756906890133No Hit
CTTGTA891790.4110111670193346No Hit
TTACGT890850.4105779366657781No Hit
CGTCTC890100.41023227414964253No Hit
TTAGCG887530.4090478039276848No Hit
ACCTGC883180.4070429613340988No Hit
TTCCAT857460.39518904144742445No Hit
GAGTGA808360.3725596687244187No Hit
CTGTTC799760.3685960718727313No Hit
ACGAAC798930.368213538688208No Hit
TTAGGC798660.3680891001823992No Hit
CTCGTC797460.36753604015658237No Hit
AGTAGG788400.3633604369616652No Hit
ACAGCC787390.36289494477326933No Hit
AGACGG773760.3566131046466997No Hit
TCTCTG773750.3566084958131513No Hit
GTTACA771560.3555991612660355No Hit
GGATTC757210.34898548512397576No Hit
ACCGCT749400.34538598612261784No Hit
TACGAA732220.33746801008634003No Hit
TGCATA713200.3287020086771431No Hit
AAGAGG712660.32845313166552553No Hit
TTGAAT710860.32762354162680024No Hit
CCGTCG690430.3182076946872685No Hit
CGTGAA684370.3154147415568935No Hit
CAACGG674010.3106399900006747No Hit
ACGCTA672480.30993483846775827No Hit
AACTAG668600.3081466110509505No Hit
AATTGC662740.3054458345915449No Hit
GAGTCG658790.30362534533989777No Hit
TAATGA656250.30245470161858545No Hit
ATCCGG654490.3016435469140541No Hit
CGTCAG654340.301574414410827No Hit
AGTTAG651430.30023324384822114No Hit
CAGTCC644590.29708080170106516No Hit
TGTGAT635140.29272545399775757No Hit
AAGGCG621720.28654039937570586No Hit
GAGATA614900.2833971748956468No Hit
GACTTG609420.28087153411108323No Hit
ATTGCT598290.27574190237163204No Hit
GTAAGA587110.2705892264644384No Hit
TGCGAC563520.259716988123589No Hit
CGTACT559050.25765683952742124No Hit
TCATGT553750.25521415774673023No Hit
GATTCA547850.2524949459531307No Hit
TTCTAC543780.25061915069890195No Hit
GCCATA542110.24984947549630687No Hit
ATCCAC538670.2482640367556319No Hit
ATGTGA529120.24386260071683954No Hit
TCTTCA527390.2430652725129536No Hit
TCGGAC522420.24077468223936216No Hit
AGTGCA520870.24006031303934874No Hit
CCACTT519330.23935055267288377No Hit
AGAACG518720.2390694138264269No Hit
ATTCGA506420.23340054856180428No Hit
GCATAA504190.23237277868049466No Hit
CGACAA492840.227141752602977No Hit
GCTCAA485760.22387869845065766No Hit
TTCACA484940.2235007740996828No Hit
TATTCT478860.22069860330221078No Hit
CCGGAA473560.21825592152151974No Hit
AGTCAA468860.21608976975373714No Hit
AGGTCA458070.211116838354934No Hit
GTGGTG453690.20909816926070254No Hit
GCCTGG448140.20654026664129965No Hit
CTGAGG441180.20333251849156195No Hit
CTACCG439560.20258588745670922No Hit
GGACTA406870.18751961058674876No Hit
ATTGAA405740.18699881239577124No Hit
TATAAG405690.18697576822802886No Hit
CGAATT386230.17800697814269908No Hit
CGCTTG383700.17684094325493524No Hit
CGAAGC378470.17443052330908349No Hit
CCAGCG376510.17352719193358265No Hit
GCGCAG370350.17068815046772284No Hit
GTCTGC363480.16752188181992145No Hit
TGATAA351280.16189910489078355No Hit
TTCTGT348330.16053949899398381No Hit
CTGTAT346350.15962694995138602No Hit
GAACCG339460.15645146363648765No Hit
ATTCAT334370.15410556736031455No Hit
CACATT325940.15022032067895122No Hit
CTAGTT325420.1499806613344306No Hit
ATCAGC314380.14489250909691567No Hit
CTGGAG303180.13973061552262514No Hit
ATACCT302160.1392605145006808No Hit
GACCAT290420.13384974391477272No Hit
AACCTT282030.1299829325676033No Hit
AATCGG271280.12502843650299408No Hit
ACTAAT233290.10751947785234256No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)