FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23898980
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTT3451231.4440909193614122No Hit
AGTTCC3132121.310566392373231No Hit
AATTGC3067771.283640557044694No Hit
TCTCGC3007521.2584302761038337No Hit
TATTGG2876611.203653879789012No Hit
CTTCAC2819241.17964867119852No Hit
CTCCGC2498091.0452705512954945No Hit
TACTCG2471781.0342617132614027No Hit
TAGAGC2425971.0150935311883604No Hit
GGCAGG2389260.9997330430001614No Hit
CTTAGC2383320.9972475812775274No Hit
GTCTCA2327900.9740583070909301No Hit
ATTGCT2290120.9582501010503378No Hit
GATTCA2234750.9350817482587123No Hit
TGACAC2179400.9119217640250756No Hit
AACTCT2178620.9115953902635174No Hit
GTAAGA2126670.8898580608879542No Hit
GGTACC2101120.8791672280574317No Hit
TTCTAC2084600.8722547991587926No Hit
ATTCGA2056080.860321235466953No Hit
GGCTAC2054500.8596601193858482No Hit
GTGGTG2052300.8587395780070948No Hit
GCCTGG2047800.8568566524596447No Hit
TACAGT2027960.8485550429348867No Hit
ACTTGA1988580.8320773522552009No Hit
CTGAGG1965140.8222694022924828No Hit
ATCCAC1953230.8172859260102314No Hit
TTACGT1951060.8163779374684609No Hit
TCATGT1937810.8108337678009688No Hit
CAGATC1902810.7961887913208012No Hit
CGAATT1892120.7917157970758584No Hit
ACCGCT1886730.7894604706979126No Hit
ACAGTG1884810.7886570891310005No Hit
TAGCTT1884650.7885901406670913No Hit
GGCCGT1875650.784824289572191No Hit
CCACTT1860670.7785562396386791No Hit
GAGAAG1860450.7784641855008038No Hit
CAAGGA1857210.7771084791066396No Hit
CGAGCC1825880.7639991330173923No Hit
GGTAAT1792530.7500445625712896No Hit
CGTGAA1792200.7499064813644767No Hit
AGAGGT1780980.7452117203328343No Hit
ACACGA1776360.7432785834374521No Hit
CAGGAC1760530.736654869789422No Hit
TCCAGA1748970.7318178432719723No Hit
GATCAG1712370.7165033821527111No Hit
CCTGAC1708960.7150765430156434No Hit
TCCACG1687540.7061138174097806No Hit
TCAAGC1639570.6860418310739622No Hit
AAGCGC1611170.6741584787300546No Hit
GAAGAT1607050.6724345557843892No Hit
TATTCT1588560.6646978239238662No Hit
GAGATA1582750.6622667578281584No Hit
CGCTTG1577060.6598859030803825No Hit
TGTGAT1561110.6532119780844203No Hit
AGTCAA1555480.6508562290106105No Hit
AATCGG1549690.6484335314728913No Hit
GGATTC1547420.6474837001411776No Hit
CATACG1530020.640203054691037No Hit
CTTGTA1527820.6392825133122836No Hit
TAAGTC1525470.6382992077486151No Hit
ATCCGG1519580.6358346674209526No Hit
CTATGC1508260.631098063599367No Hit
ACGCTA1508110.631035299414452No Hit
GCCAAT1487770.6225244759399774No Hit
CAGCCT1486450.6219721511127253No Hit
AAGCAT1471960.6159091308499358No Hit
GTACGC1440360.6026868092278416No Hit
GTCGAG1410760.5903013434046139No Hit
TAATGA1408400.5893138535619511No Hit
TCTTCA1378850.5769493091336952No Hit
CTGGAG1374680.5752044647930581No Hit
AGGTGT1354400.5667187469925495No Hit
AAGGCG1349770.5647814258181729No Hit
TGACCA1348260.5641495996900286No Hit
GGATCG1345330.5629236059446888No Hit
GGACTA1335500.5588104596932588No Hit
CGTCTC1308160.5473706409227507No Hit
TTAGCG1290070.539801280222001No Hit
CACATT1269120.5310352157288721No Hit
GATCCT1263160.5285413854482492No Hit
CCGCTC1251220.5235453563290149No Hit
AACTAG1250580.5232775624733775No Hit
TTGAAT1240580.5190932834790438No Hit
CTGTTC1226470.5131892658180391No Hit
AACCTT1212500.507343828062955No Hit
CGTCAG1208820.5058040133930403No Hit
GACTTG1200540.5023394303857319No Hit
TTAGGC1196000.5004397677223045No Hit
TCCTCC1176550.49230134507832546No Hit
CATGAG1166690.4881756459899125No Hit
CCTCGG1157260.4842298708982559No Hit
GCGTAT1145430.4792798688479592No Hit
ACGAAC1126280.4712669745738102No Hit
TGCGAC1116060.46699064144160124No Hit
CTCGTC1112940.4656851463953692No Hit
AGTTAG1107150.46326244885764994No Hit
AACATA1076910.45060918917878506No Hit
TGCATA1074300.4495170923612639No Hit
GAGCCA1054110.4410690330717043No Hit
CGTACT1030850.4313364001308843No Hit
TTCACA1026860.4296668728121451No Hit
AGTAGG970450.406063355005109No Hit
ACCAGG970430.4060549864471203No Hit
ATGTGA958990.4012681712776026No Hit
CAAGCT940030.39333477830434604No Hit
CCAGCG932620.39023422756954484No Hit
AGAACG930810.3894768730715704No Hit
CTAGTT920490.3851586971494181No Hit
AGGTCA917700.383991283309999No Hit
TACGGC906030.37910822972361163No Hit
TGCGGA894220.3741665962313036No Hit
AAGTTG882090.36909106581117684No Hit
GCTGTC874000.36570598410476096No Hit
TGGCAT870340.36417453799283483No Hit
CCTGCT869480.36381468999932215No Hit
TGATAA868210.3632832865670418No Hit
CAGTCC850250.3557683214932185No Hit
TATGGT844510.353366545350471No Hit
AGGACT844470.3533498082344937No Hit
GTTGGC835390.34955048290763874No Hit
AGTGCA827590.3462867452920585No Hit
ATACCT827550.34627000817608117No Hit
ATCAGC819310.3428221622847502No Hit
GACACG818790.34260457977704484No Hit
GTTACA815800.3413534803577391No Hit
ACGTAA815030.3410312908751754No Hit
GCGCAG808540.33831569380785287No Hit
ACTAAT805060.3368595647178248No Hit
GACTAA781890.32716459028795375No Hit
CTACCG779190.32603483495948365No Hit
GCTCAA775780.32460799582241584No Hit
ACAGCC769040.321787791780235No Hit
CATTAA762970.31924793443067445No Hit
CCTTAT760130.3180595991962837No Hit
CTGTAT755730.31621851643877685No Hit
GAGTGA753840.3154276877088478No Hit
TCGGAC749460.3135949735093297No Hit
GAGCAC744780.3116367309399815No Hit
CCGGAA743220.3109839834168655No Hit
ATTCAT733070.30673694023761683No Hit
TTCTGT728420.3047912505052517No Hit
GCTTCT726180.30385397201052095No Hit
GAGTCG719820.3011927705701248No Hit
ATTGAA698630.29232628338113176No Hit
ACTAGC692410.2897236618466562No Hit
CAACGG688140.28793697471607577No Hit
TCAATG685230.2867193495287247No Hit
GCGGCT680210.28461884147356914No Hit
CGTTGC665180.2783298701450857No Hit
AATGTA663480.27761854271604897No Hit
GCATAA649750.2718735276568289No Hit
TTAGTA627370.26250911126751014No Hit
CGACAA613300.2566218307224827No Hit
AGCCTA608700.2546970623850892No Hit
CCATGA608330.2545422440622989No Hit
TTCCAT605880.25351709570868713No Hit
GAATAC592290.2478306605553877No Hit
GGCGTT590270.24698543619853233No Hit
CCGTCG578810.24219025247102596No Hit
TACGAA564780.23631970904197586No Hit
TATAAG549520.2299344992966227No Hit
CAGTAG540310.22608077834284143No Hit
GACCAT539450.2257209303493287No Hit
CACCTA529970.22175423386270043No Hit
CATAAC520850.21793817141986813No Hit
GCACAT503400.21063660457475591No Hit
AGATGC500060.2092390553906485No Hit
TCGTGG500010.2092181339956768No Hit
GCCATA499610.20905076283590346No Hit
GAACCG498190.2084565952187081No Hit
ATGGCC490280.2051468305341902No Hit
AACAAT484510.20273250155445963No Hit
AGGCTT478500.20021774987886515No Hit
TTCGTT472150.19756073271746327No Hit
CCGACT444780.18610836110997206No Hit
CGAAGC441750.18484052457468897No Hit
ACCTGC426690.1785390004092225No Hit
AAGAGG405830.1698105944270425No Hit
GGTGGT358670.150077534689765No Hit
ATGCCG350350.14659621456647942No Hit
GGCCAG329430.1378427029103334No Hit
AGACGG268580.11238136522981315No Hit
GTCTGC248280.10388727887131585No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)