FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25546914
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGA9870683.8637465174854393No Hit
TATTCT8665343.391932191888226No Hit
ACCGCT8632003.3788816919335147No Hit
GAGATA8019083.1389623028440927No Hit
AGTCAA7071882.76819344990162No Hit
AATCGG6738362.6376414779491566No Hit
ATCCAC6556762.5665565711772467No Hit
CGAATT6368822.492989955655701No Hit
GGATTC6223892.4362590330871274No Hit
GGACTA5526822.163400244741889No Hit
CACATT5195762.0338112070992214No Hit
CTGGAG3204171.2542297672431197No Hit
TATTGG2673761.0466078212029837No Hit
TAATGA2262430.88559815874434No Hit
ACACGA2077780.8133193700029678No Hit
CGAGCC2049660.8023121696812382No Hit
CTTGTA1982440.7759997939477151No Hit
TAAGTC1970200.7712086086014146No Hit
GAAGAT1930320.7555981125548079No Hit
AAGCAT1676180.656118386745264No Hit
GGCTAC1652160.6467160769398606No Hit
TCAAGC1643460.6433105775515586No Hit
GGATCG1629510.6378500354289367No Hit
GATCCT1621010.6345228233828947No Hit
ACTTGA1617420.6331175655893311No Hit
GGTACC1615810.6324873524841396No Hit
CAGGAC1614410.631939341088321No Hit
ATCCGG1603010.6274769625795116No Hit
ACGCTA1551820.6074393173281125No Hit
TAGCTT1482960.580484985388059No Hit
AATTGC1467810.5745547192118782No Hit
TCCACG1446450.5661936310585302No Hit
GATTCA1369880.5362213220743609No Hit
CCGCTC1361450.5329215105981098No Hit
TCTTCA1353660.5298722186170901No Hit
GGAGGC1346790.5271830484104656No Hit
TTACGT1345990.5268698990414263No Hit
CATACG1328770.5201293588728564No Hit
AACCTT1301730.5095449101993298No Hit
CTGTTC1300290.5089812413350591No Hit
GAGAAG1276630.4997198487457233No Hit
CCTGCT1275210.4991640086156786No Hit
GTACGC1268780.4966470705620256No Hit
CCACTT1267660.4962086614453707No Hit
GAGCCA1260730.4934960050360681No Hit
AGTGCA1248600.48874787772801054No Hit
ACAGTG1244740.48723693202239615No Hit
CAGATC1237380.484355957827235No Hit
TTAGTA1214880.4755486318230061No Hit
GCCAAT1188990.4654143353674733No Hit
AGCCTA1173360.45929617956986896No Hit
CCGACT1173310.45927660773430407No Hit
CAAGCT1166290.45652872202098466No Hit
ATTCGA1162730.45513520732876No Hit
TGGCAT1143140.4474669621544113No Hit
GATCAG1142690.44729081563432666No Hit
TGCATA1131300.44283235149263034No Hit
ATTGCT1118750.43791982076582714No Hit
GTTACA1116950.43721523468548884No Hit
AACAAT1110640.43474526903719174No Hit
TTGAAT1108400.4338684508038818No Hit
TCGGAC1093980.4282239334269493No Hit
CATAAC1085840.42503763859697496No Hit
ATGTGA1080720.4230334826351238No Hit
CTATGC1079740.4226498746580507No Hit
AGGCTT1069000.41844584437869875No Hit
CACCTA1056460.4135372280190085No Hit
GAATAC1045900.4094036563476904No Hit
TTAGGC1031830.4038961418197125No Hit
GTCTCA1029320.4029136356743519No Hit
CTGAGG1026650.4018684996551834No Hit
TCTCTG1019250.39897186799157036No Hit
ACGAAC1017390.3982437957085541No Hit
CGTACT1013490.39671719253448773No Hit
TACTCG985110.3856082186678203No Hit
GGCGTT981370.3841442453675618No Hit
AACATA974070.38128675737507867No Hit
TTCTAC966020.3781356918491212No Hit
CCGTCG957110.37464799075144656No Hit
AGATGC950830.3721897682044884No Hit
AAGGCG941340.3684750338142603No Hit
TGTGAT926460.36265045555013026No Hit
CCATGA923200.3613743718712953No Hit
CAGTAG922000.36090464781773646No Hit
GTACTT910030.3562191503834866No Hit
CGACAA909290.35592948721712536No Hit
AGTAGG904160.3539214168881611No Hit
TGCGAC898790.3518194017484852No Hit
CCTTAT882200.3453254667080337No Hit
CGTGAA878460.3438614934077752No Hit
ATGGCC877220.3433761118857644No Hit
ATTCAT869820.3404794802221513No Hit
CAGCCT868870.3401076153464172No Hit
CCGGAA868770.3400684716752873No Hit
ACGTAA864840.338530125399882No Hit
AATGTA856070.3350972254417892No Hit
CGTCAG851880.3334571056214461No Hit
GCTTCT851460.3332927022027005No Hit
GGCCAG850510.33292083732696637No Hit
TGACCA848910.3322945385888879No Hit
ACCTGC848850.33227105238620996No Hit
CATTAA847980.33193050244737976No Hit
ACCAGG845570.330987139973149No Hit
CTAGTT843370.33012597920829106No Hit
AGAACG839300.3285328317933039No Hit
AGGACT838740.3283136272349764No Hit
GACCAT834290.32657173386969557No Hit
CCTCGG833740.3263564436784811No Hit
GCGTAT816410.3195728454716683No Hit
TGATAA813850.3185707674907427No Hit
TAGAGC808740.31657052589600454No Hit
TACAGT808360.31642177994571086No Hit
AGACGG805510.31530618531850857No Hit
GGCAGG803200.3144019665154077No Hit
TCATGT796800.3118967715630937No Hit
TACGGC791810.3099435023737114No Hit
AAGTTG791340.3097595271194008No Hit
CTTCAC788930.3088161646451701No Hit
GTGGTG787010.30806460615947584No Hit
GGTGGT783310.30661629032766935No Hit
ATGCCG777510.30434595740213477No Hit
CGAAGC772440.3023613732758485No Hit
ACTAGC768980.30100700225475374No Hit
GACTAA761460.2980633981857848No Hit
CTCGTC758000.29670902716469No Hit
AGAGGT755090.29556994633480976No Hit
ATCAGC743400.2909940511797237No Hit
GCACAT743240.29093142130591587No Hit
TGCGGA742690.29071613111470135No Hit
CAGTCC741030.2900663461739449No Hit
GAGCAC737910.28884506363469187No Hit
GACTTG736120.2881443919214665No Hit
GCATAA735160.2877686126786194No Hit
CAAGGA733370.2870679409653941No Hit
GCCTGG732630.2867782777990328No Hit
CGCTTG729230.285447392980616No Hit
TTCGTT720600.2820692941621051No Hit
TCGTGG718320.2811768184603432No Hit
CTCCGC712880.2790474027508763No Hit
TACGAA697060.2728548739781251No Hit
AGTTAG689900.27005218712522383No Hit
TTAGCG684140.2677975116681412No Hit
TATGGT682330.2670890112206899No Hit
AACTCT682200.26703812444822106No Hit
GAACCG681900.2669206934348313No Hit
ATTGAA681420.26673280381340775No Hit
GCTCAA671130.2627049200541404No Hit
AAGCGC663630.25976914471939744No Hit
TTCACA662580.2593581361725334No Hit
GCGCAG659430.25812511053194137No Hit
GAGTGA656790.25709171761411187No Hit
TCTCGC653600.2558430345050678No Hit
GTCGAG653300.25572560349167806No Hit
GGTAAT652280.255326338046153No Hit
CTGTAT646850.2532008367037991No Hit
GAGTCG644410.25224573112822946No Hit
CGTTGC637020.24935301383172934No Hit
TCAATG635350.24869931452385993No Hit
CAACGG631330.24712573894443765No Hit
GACACG629500.24640940976276038No Hit
CCTGAC624790.2445657428525418No Hit
AGTTCC622420.24363803784676302No Hit
CATGAG618430.24207620536867974No Hit
GGCCGT609170.23845150142205043No Hit
TGACAC608560.23821272502815802No Hit
GCGGCT604650.23668220748697869No Hit
AGGTGT600040.23487768424789No Hit
ACTAAT599770.23477199633583926No Hit
AAGAGG596540.23350765575834326No Hit
GTCTGC591930.2317031325192546No Hit
ACAGCC591080.23137041131465036No Hit
TCCAGA585010.22899439047706507No Hit
GTTGGC583670.22846986528392432No Hit
AACTAG566670.22181544119184027No Hit
TTCCAT564050.2207898770082367No Hit
GCCATA561100.21963513870990448No Hit
TCCTCC550940.215658141723106No Hit
TTCTGT524680.20537901368439257No Hit
ATACCT500090.1957535849535486No Hit
CGTCTC492150.19264557746583405No Hit
TATAAG459940.1800374009948912No Hit
CTTAGC423850.16591045008410799No Hit
GCTGTC359360.14066669657243142No Hit
CCAGCG324510.12702512718365905No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)