FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29440602
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAATT12134224.12159370925907No Hit
ACCGCT10423893.540651104892488No Hit
GTAAGA8945493.0384874602767975No Hit
GAGATA8046562.733150633264904No Hit
GGATTC8022732.7250563694315764No Hit
AGTCAA7445002.528820572351068No Hit
ATCCAC7253792.463872851513023No Hit
GGACTA6716892.2815056567117753No Hit
AATCGG6263222.127408943607879No Hit
TATTCT5477481.8605190206368742No Hit
GGTACC5283841.7947459090680278No Hit
CACATT4794041.6283770284316874No Hit
CTGGAG4541741.5426790525546998No Hit
GAAGAT4481141.5220952343297873No Hit
TATTGG3738671.2699027010385182No Hit
CGAGCC3689291.2531299461879208No Hit
GATCCT3233331.0982553957286607No Hit
GGATCG3216131.0924131238892465No Hit
CAGGAC3051541.036507337723597No Hit
ACACGA2830170.9613152611485323No Hit
CTTGTA2775060.9425962145746885No Hit
TCCACG2753060.9351235412917168No Hit
GAGCCA2493750.8470444999732003No Hit
TCAAGC2345040.7965326252499865No Hit
GATCAG2326960.7903914464792534No Hit
ACTTGA2182720.7413978831003524No Hit
GGCTAC2025950.6881482926198317No Hit
TAAGTC1927760.6547963930900598No Hit
ATCCGG1876000.6372152308570321No Hit
ACAGTG1746270.5931502351752183No Hit
TAGCTT1716900.5831742163424511No Hit
TGACCA1702190.5781777152518824No Hit
CAGATC1692700.5749542757311824No Hit
AACCTT1669240.5669856886757955No Hit
GCCAAT1662250.5646114165736149No Hit
CTGTTC1650780.5607154364574475No Hit
TAATGA1557460.5290177150589516No Hit
AATTGC1532380.520498867516364No Hit
GGAGGC1510080.51292429414317No Hit
CTATGC1506490.511704889730176No Hit
TCATGT1446570.4913520450431007No Hit
GAGAAG1443770.49040097753435885No Hit
TGGCAT1383290.4698579193455351No Hit
AGCCTA1369960.4653301586699891No Hit
ACGAAC1344570.45670601436750513No Hit
GATTCA1342930.45614896054095627No Hit
ATTGCT1340550.45534055315852573No Hit
TTAGGC1334310.4532210312819011No Hit
TGCATA1324650.4499398483767418No Hit
CAGCCT1321140.4487476173211403No Hit
TTAGTA1311140.4453509476470624No Hit
TAGAGC1305860.4435575060591492No Hit
GGCGTT1302870.44254190182659986No Hit
CCTTAT1302040.44225997824365143No Hit
CCACTT1297020.44055485006726425No Hit
CGTACT1270140.43142460198334254No Hit
CATACG1240290.42128554300621973No Hit
ACGCTA1216220.413109759100714No Hit
GAATAC1216120.41307579240397324No Hit
CAAGCT1211020.4113434908701935No Hit
AACAAT1209800.410929097169956No Hit
CCATGA1201410.40807929131340454No Hit
GTGGTG1197010.40658475665681026No Hit
GTACGC1190370.4043293679932224No Hit
AAGCAT1169360.39719296500798457No Hit
GTCTCA1157430.39314073808680955No Hit
TACAGT1156260.3927433277349424No Hit
TTCTAC1155730.39256330424221625No Hit
CCGGAA1138530.3867210324028021No Hit
CATAAC1130360.3839459532790804No Hit
ATTCGA1121370.38089234724208426No Hit
CCTGCT1113680.3782803082627183No Hit
CTGAGG1107710.3762524964672937No Hit
CGTGAA1107430.37615738971641954No Hit
TTACGT1104710.3752334955650703No Hit
CTCGTC1094320.37170435577370325No Hit
TGATAA1062390.3608587895043722No Hit
AGAACG1060510.36022021560564554No Hit
CAGTAG1049020.35631744215012995No Hit
AGATGC1046260.3553799613200844No Hit
GCTGTC1039760.3531721260319337No Hit
TGCGAC1036550.3520817950665547No Hit
GTACTT1032980.35086918399290884No Hit
CCGACT1023000.34747930765817897No Hit
AGGCTT1021010.34680337039303744No Hit
CACCTA1002700.3405840682198007No Hit
TGTGAT991760.3368681115963593No Hit
GGCCAG978600.3323980943052727No Hit
CATGAG974170.33089336963965615No Hit
TTGAAT954140.3240898402824779No Hit
GCGTAT950850.32297233595970626No Hit
AGTGCA949930.3226598423496911No Hit
CCGCTC932970.3168990905824548No Hit
ATGTGA923490.31367904773142885No Hit
ATCAGC918120.311855036116449No Hit
ATGGCC914080.3104827815681215No Hit
CTCCGC913880.3104148481746399No Hit
TCCAGA908660.3086417866047712No Hit
GGTGGT905380.3075276789516736No Hit
TTCACA891250.30272818470220136No Hit
TCTTCA885140.30065281953133977No Hit
CTAGTT878700.2984653642612335No Hit
ATTGAA873760.29678740944223897No Hit
GCGCAG871070.295873705299912No Hit
ACTAAT862600.29299672608596794No Hit
GGCCGT860250.2921985087125596No Hit
TTCCAT859770.29203546856820384No Hit
CGCTTG854200.29014352355974243No Hit
TCTCGC837540.28448467188272847No Hit
GGCAGG834130.2833264075238679No Hit
TCGGAC834120.2833230108541938No Hit
TACGAA831740.28251460347176327No Hit
TGCGGA830520.2821002097715257No Hit
ACCAGG830180.2819847230026071No Hit
GCTCAA829400.281719782768029No Hit
AACTCT829070.28160769266878444No Hit
ATGCCG822720.2794508074257449No Hit
CAGTCC821220.2789413069746332No Hit
TCCTCC816480.27733128554912023No Hit
GACACG816250.27725316214661644No Hit
CTTAGC815140.2768761318127938No Hit
GCACAT815020.2768353717767048No Hit
AACATA814200.27655684486343046No Hit
AGTTCC812670.2760371544032965No Hit
AAGGCG810950.2754529272193551No Hit
TCTCTG809450.27494342676824335No Hit
ACCTGC806390.2739040458479755No Hit
GACTAA799890.27169621055982485No Hit
CGTTGC797810.2709897032676166No Hit
ACTAGC793770.2696174487192891No Hit
TCAATG793220.2694306318872148No Hit
AGAGGT779380.2647296410582909No Hit
CGACAA776850.2638702836307491No Hit
CGTCAG771130.2619273885771765No Hit
CATTAA770900.2618492651746727No Hit
ATTCAT770080.2615707382613983No Hit
GTTGGC768980.2611971045972497No Hit
AGTTAG767300.26062646409200463No Hit
CTTCAC756500.2569580608440004No Hit
AGACGG755010.2564519570625628No Hit
TACTCG749920.2547230521984571No Hit
ACGTAA744750.2529669739769588No Hit
CCTCGG732750.24889097036806515No Hit
TACGGC732440.24878567360816875No Hit
GACTTG731240.2483780732472794No Hit
GCCATA724300.24602078449346923No Hit
CCTGAC724130.24596304110900993No Hit
AAGAGG721260.24498819691254956No Hit
GCTTCT720720.24480477675014933No Hit
AGGACT720050.24457719988198612No Hit
GCCTGG719930.24453643984589718No Hit
ATACCT718800.24415261617272635No Hit
TTAGCG717670.24376879249955555No Hit
CCGTCG717380.2436702890790073No Hit
GACCAT711140.2415507672023826No Hit
TTCTGT709620.24103447341192277No Hit
AAGTTG706260.23989319240143256No Hit
GTCTGC703010.2387892747573572No Hit
CGAAGC691500.23487970796249344No Hit
CAAGGA673270.22868757914664925No Hit
GAGCAC660190.22424473521295524No Hit
AATGTA652810.22173799299348568No Hit
GGTAAT650330.2208956189143143No Hit
TGACAC631880.2146287633656404No Hit
TCGTGG628840.21359617578472068No Hit
CCAGCG626180.21269266165141595No Hit
TATGGT614150.20860646803350014No Hit
AGTAGG599500.20363034696097587No Hit
CTGTAT594200.20183011203371454No Hit
AGGTGT590070.20042728745832034No Hit
TTCGTT589400.20019971059015712No Hit
GTCGAG586190.19910937962477807No Hit
GTTACA585430.1988512327295481No Hit
GAGTCG583420.19816850212505846No Hit
GAACCG576590.19584857673766318No Hit
ACAGCC573670.1948567491928324No Hit
GCATAA563620.19144309617038402No Hit
TATAAG538260.1828291418769222No Hit
GAGTGA537690.18263553170549976No Hit
GCGGCT519270.1763788661658481No Hit
AACTAG495940.16845443581622416No Hit
CGTCTC481180.163440951377285No Hit
AAGCGC470500.1598133081653697No Hit
CAACGG455420.1546911302968601No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)