FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26441076
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTC5881702.224455615951484No Hit
TCAAGC5028381.9017304742061178No Hit
AACCTT4746961.7952975892509062No Hit
CATACG4465851.6889819461204985No Hit
TAAGTC4360861.6492747874556994No Hit
CTATGC4067511.5383299832427393No Hit
ATCCGG4019651.5202293582908655No Hit
ACGAAC3965331.4996855649898666No Hit
AAGCAT3896191.4735368560644053No Hit
TGGCAT3700911.3996820704270885No Hit
TAATGA3041701.1503692209802656No Hit
TGCATA2867631.0845360453560966No Hit
GGTACC2740451.0364366412319983No Hit
TTACGT2527000.9557099718634748No Hit
GAGAAG2501210.9459562084387186No Hit
ACTTGA2435590.9211387615239258No Hit
ACTAAT2367690.8954590198976774No Hit
CTTAGC2317910.8766322520308931No Hit
CAGCCT2285180.8642537845282847No Hit
ATTCGA2276430.8609445394733557No Hit
TTCTAC2122220.8026224046252883No Hit
GATCAG2119300.8015180622755292No Hit
ACAGTG2115180.7999598806039512No Hit
TGACCA2020700.7642275979994158No Hit
TGATAA2010290.7602905418826374No Hit
GGCTAC1982770.7498824934355924No Hit
GTACGC1962230.7421142770437935No Hit
GTGGTG1952340.7383738846331367No Hit
CTTGTA1924770.7279469262143492No Hit
TGTGAT1915700.7245166573402686No Hit
TTGAAT1860300.7035644086496329No Hit
CATTAA1837340.6948809496254994No Hit
GGACTA1830950.6924642552368141No Hit
GGAGGC1827070.6909968414295999No Hit
ACGCTA1818610.6877972742107772No Hit
GCTTCT1804700.6825365200720274No Hit
ATTGCT1801440.6813035899144195No Hit
TATTGG1791320.6774762116337475No Hit
CAGATC1787480.6760239258039271No Hit
ATTGAA1772700.6704341381568587No Hit
CTAGTT1772410.6703244603207524No Hit
TGCGAC1757710.6647649286284718No Hit
TGACAC1744010.6595835963710402No Hit
AAGGCG1743860.6595268664558129No Hit
TCATGT1732030.655052767141549No Hit
AGTGCA1728770.6538198369839412No Hit
GACTTG1711640.6473412806649775No Hit
CCACTT1711330.6472240388401743No Hit
CAGTCC1703980.644444272994034No Hit
GTCTCA1672250.6324440049262745No Hit
CTCGTC1661360.6283254130807687No Hit
AATTGC1643240.6214724393213045No Hit
GATCCT1617610.6117791878061241No Hit
TCTCTG1606700.6076530319719213No Hit
TAGCTT1585290.5995557820718037No Hit
CCTCGG1559380.5897566347148656No Hit
AACTAG1550980.5865797594621338No Hit
GTAAGA1549380.5859746403663755No Hit
GACCAT1515500.5731612435136906No Hit
TTCACA1505930.5695418749221854No Hit
GAGCAC1500760.5675865838440161No Hit
GAACCG1499500.5671100525561062No Hit
ATTCAT1495660.565657766726286No Hit
GCCAAT1492970.5646404102465421No Hit
CGTGAA1491120.5639407412920715No Hit
CGACAA1490790.5638159354785713No Hit
ACACGA1465660.5543117836808155No Hit
GCCATA1461410.552704436082707No Hit
CTGTAT1460660.5524207865065702No Hit
TTCCAT1457520.5512332402811444No Hit
GTCTGC1457460.5512105483150535No Hit
CGTACT1442120.5454089689844694No Hit
GAAGAT1437550.5436805975672094No Hit
TCGGAC1428200.5401444328513711No Hit
GGATCG1408740.5327846718492092No Hit
TACTCG1407540.5323308325273903No Hit
TTCTGT1405550.5315782156520408No Hit
TACGAA1404740.5312718741098131No Hit
AGTTAG1396110.528008012987066No Hit
CCGTCG1393940.5271873202134436No Hit
CGAGCC1382860.5229968704753165No Hit
GATTCA1362210.5151870521456843No Hit
TTAGGC1362180.5151757061626387No Hit
CAAGGA1361920.515077374309578No Hit
CGAAGC1357160.5132771449996967No Hit
GTACTT1344480.5084815761658111No Hit
CGAATT1336710.5055429665570342No Hit
AGTCAA1330700.5032699879535916No Hit
CAGGAC1297850.49084613651880127No Hit
CGTTGC1290430.4880398967122216No Hit
GTTACA1288190.4871927299781597No Hit
GAGCCA1285770.48627748734582515No Hit
CCTGAC1278870.4836679112453669No Hit
TACAGT1243840.4704195850426056No Hit
TCCACG1239570.46880467345580035No Hit
GGCCGT1235490.4672616197616164No Hit
GGCAGG1228050.46444781596633966No Hit
CTACCG1226910.46401666861061175No Hit
ACTAGC1225270.4633964215374594No Hit
TATTCT1222550.46236771907467No Hit
CTCCGC1218160.46070742355568284No Hit
TCCAGA1213010.4587596964662104No Hit
GCTCAA1212520.4585743787431344No Hit
GCGCAG1179440.4460635414383287No Hit
ATACCT1157730.4378528317077565No Hit
AGAACG1157000.4375767461203168No Hit
GAGATA1133490.4286852774070163No Hit
CCGGAA1120740.42386323461269126No Hit
CTGAGG1116350.4222029390937041No Hit
TAGAGC1108160.4191054857222906No Hit
GGTAAT1104770.41782338963815235No Hit
AGGTCA1098830.41557688499514917No Hit
CTTCAC1076410.40709765366583417No Hit
CCAGCG1071780.4053465902824832No Hit
GCACAT1054400.39877348410480723No Hit
CGTCAG1050820.39741953012804776No Hit
AATGTA1042120.39412919504486127No Hit
ACCAGG1029930.3895189439340517No Hit
TCGTGG1027740.3886906871717324No Hit
CAACGG1026700.38829735975948937No Hit
AACTCT1015090.38390646432089226No Hit
TCTTCA995300.37642189750523014No Hit
GTCGAG982090.37142588297087453No Hit
ATGTGA981850.3713351151065108No Hit
AAGCGC973180.3680561260063698No Hit
AGATGC969350.36660762217089804No Hit
GGATTC965070.36498892858974424No Hit
TCAATG959920.3630412015002718No Hit
AAGTTG954140.3608552087668444No Hit
CACATT951890.36000426003843417No Hit
AGAGGT948110.35857466617470485No Hit
AGGTGT925370.3499744110262381No Hit
TCTCGC923400.34922935813958556No Hit
ACAGCC920840.34826116758637204No Hit
TTAGTA917500.3469979814739763No Hit
AACATA909420.34394213004039625No Hit
TCCTCC889320.3363403213999309No Hit
ACGTAA866330.3276455163927519No Hit
CCATGA858610.3247258167557175No Hit
CTGGAG855580.323579872468125No Hit
AGTAGG844150.3192570529278007No Hit
GACACG824110.3116779362534263No Hit
CGTCTC821930.31085346148545545No Hit
TTCGTT815150.30828926931717904No Hit
CACCTA814540.3080585676619212No Hit
CCGCTC812750.3073815906735414No Hit
GCTGTC802010.30331972874326296No Hit
ATCCAC796130.30109591606635067No Hit
GAGTCG781020.295381322605782No Hit
ACCTGC768740.290737033545836No Hit
TATGGT766260.28979909894741046No Hit
CCTGCT763450.2887363585354847No Hit
GAGTGA761370.28794970371099876No Hit
GTTGGC732470.27701974004386204No Hit
GCATAA727760.2752384207057232No Hit
GCGTAT693590.2623153460169321No Hit
TGCGGA672770.2544412337833755No Hit
CATGAG667060.2522817150103876No Hit
GACTAA656330.24822363507445763No Hit
AGGCTT652960.24694910297901645No Hit
TACGGC595720.2253009673282585No Hit
AGGACT576090.21787691242217225No Hit
AAGAGG512850.19395958016232018No Hit
GCGGCT504560.19082430684742177No Hit
AGCCTA416430.15749359065417762No Hit
CAGTAG393750.14891602747180183No Hit
AACAAT383870.1451794170554935No Hit
CCGACT337920.12780115302418102No Hit
ATGCCG326520.12348967946690219No Hit
GAATAC319760.1209330512873228No Hit
CCTTAT313350.11850879290994057No Hit
GGCGTT313220.11845962698341021No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)