FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24224149
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATA5142772.122993051272926No Hit
GGCTAC4436321.831362579548202No Hit
AGTTAG3863541.5949125808299807No Hit
AGTGCA3645721.504994045404856No Hit
TTCCAT3581731.4785782567635297No Hit
GACTTG3084741.273415218838028No Hit
TCGGAC2854481.178361312093977No Hit
ACTTGA2838511.1717687172416253No Hit
CAGTCC2771411.144069085770567No Hit
CGAAGC2452551.01244010677114No Hit
GCCAAT2359390.9739826154470896No Hit
TACGAA2356910.9729588436728985No Hit
TAGCTT2254450.9306622081956315No Hit
ATGTGA2128680.8787429436633666No Hit
CGTACT2103160.8682080018579806No Hit
CGACAA2077600.8576565476046238No Hit
ACCGCT2063650.8518978313747988No Hit
GAACCG2037830.8412390462096315No Hit
CTTGTA2005510.8278969882492054No Hit
TCTTCA1997010.8243880930554053No Hit
CCAGCG1986640.8201072409189689No Hit
ATCAGC1970890.8136054645304567No Hit
CACCTA1970760.8135517990745516No Hit
CAGATC1964870.8111203411108476No Hit
GATCAG1937830.7999579262825703No Hit
GCTCAA1928810.7962343692651493No Hit
CCGTCG1880320.7762171542125175No Hit
CTGTAT1788280.7382220114316502No Hit
TCAAGC1765630.7288718377681709No Hit
CCTGCT1764230.7282939020891921No Hit
TATGGT1761610.7272123367471031No Hit
TTCACA1750360.7225682107553086No Hit
CCGGAA1723850.7116245858626448No Hit
AGAACG1696220.7002186124267977No Hit
CTACCG1692070.6985054459498247No Hit
ACAGTG1674360.6911945596107422No Hit
CCTGAC1663450.6866907894266997No Hit
TGACCA1639510.6768080893161613No Hit
TGCGAC1633950.6745128590482167No Hit
GTAAGA1629350.6726139275315719No Hit
AGGCTT1627380.6718006894690088No Hit
TTAGTA1626580.6714704405095923No Hit
TTAGGC1551830.6406128033641141No Hit
GTACTT1544110.6374259009057449No Hit
CCATGA1518780.6269693932282203No Hit
AACTAG1514800.6253264046551233No Hit
GTTACA1498120.6184407138512895No Hit
TGATAA1494390.6169009280780101No Hit
ACAGCC1485870.6133837766602245No Hit
AACATA1481710.6116664820712587No Hit
CAAGCT1473660.6083433519171303No Hit
ACGTAA1463820.6042812897163075No Hit
CTTCAC1445880.5968754568013928No Hit
CAACGG1435800.5927143199127449No Hit
TTCTGT1423640.5876945357296143No Hit
GCACAT1420400.5863570274439776No Hit
TAGAGC1416200.5846232204070408No Hit
CCTCGG1416140.5845984517350847No Hit
TGCATA1412250.582992616169922No Hit
GAGAAG1409150.581712901452183No Hit
TACGGC1401060.578373258850084No Hit
AGTTCC1393830.5753886338793573No Hit
TAATGA1390250.5739107697859686No Hit
GAGATA1386850.5725072117084484No Hit
CAAGGA1385820.5720820161731998No Hit
GAGCCA1372270.5664884244230829No Hit
CGAATT1365930.5638712014197073No Hit
AGTAGG1361340.5619763980150552No Hit
GACCAT1359110.5610558290406816No Hit
ATTCAT1356910.5601476444022863No Hit
GAGTCG1343610.5546572554519872No Hit
GTCTGC1330810.5493732721013234No Hit
AGTCAA1330780.5493608877653452No Hit
ATTGAA1325220.5470656574974007No Hit
TACAGT1303850.5382438821689877No Hit
CATGAG1299410.5364110004442262No Hit
TCGTGG1296480.5352014636303632No Hit
AAGCAT1296180.535077620270582No Hit
TTACGT1289140.5321714294277169No Hit
CTATGC1273180.525582962687358No Hit
AGGTCA1263960.5217768434300829No Hit
ATACCT1261270.520666381304045No Hit
CATAAC1238120.5111098020409304No Hit
GGCCGT1234380.5095658881556583No Hit
ACCTGC1227070.5065482382889901No Hit
ACGAAC1225970.5060941459697924No Hit
GGATTC1204180.4970989899376857No Hit
CGAGCC1201550.496013296483604No Hit
CTGTTC1192280.4921865366663654No Hit
CTGGAG1186310.4897220538067199No Hit
AACCTT1176230.48556091691807207No Hit
AATCGG1175400.48521828362267755No Hit
GGACTA1164860.48086725358236526No Hit
TATTCT1151250.47524889316029223No Hit
GAAGAT1150160.4747989289530873No Hit
AAGAGG1148240.4740063314504877No Hit
GGTACC1130660.46674911056731033No Hit
GAGTGA1110450.45840619623005124No Hit
ATCCAC1108900.4577663388711818No Hit
ACTAAT1083720.44737175287354775No Hit
GCATAA1047310.43234129710810487No Hit
GTACGC1046610.43205232926861536No Hit
TATAAG1039780.4292328287775971No Hit
CACATT1026800.42387453941106457No Hit
TCCTCC1020220.42115824171986393No Hit
CGAGAG1016970.41981660532223447No Hit
GCGCAG1015680.4192840788751754No Hit
CTAGTT1011830.4176947557579835No Hit
CAGCCT1011100.417393403582516No Hit
AACTCT1009670.416803083567559No Hit
CCGCTC1001900.4135955405492263No Hit
AGCCTA1001080.4132570353658245No Hit
TAAGTC994330.41047055977074776No Hit
CTATTA952180.39307056772149146No Hit
ATTGCT948540.3915679349561465No Hit
GGAGGC946230.3906143410858314No Hit
CTTAGC943960.38967725966348704No Hit
ACCAGG942460.3890580428645811No Hit
GATCCT931390.3844882228886555No Hit
CTCCGC928590.3833323515306977No Hit
GGCAGG913930.37728053934939054No Hit
TCCACG908560.37506374320930735No Hit
GTCTCA908180.37490687495358455No Hit
ACACGA907110.374465166970365No Hit
ATCCGG900140.3715878729114488No Hit
AGATGC894120.36910274949183974No Hit
TATTGG871660.3598310099562218No Hit
GATTCA859340.35474517598120786No Hit
TGGCAT840000.3467614073873142No Hit
TGCGGA838240.3460348596765979No Hit
TTGAAT833460.3440616221440844No Hit
GGATCG829850.34257137371471746No Hit
TGTGAT826330.3411182782932849No Hit
CAGGAC820070.3385340801858509No Hit
CGTCAG808240.33365052369847953No Hit
GAATAC802880.3314378556703891No Hit
GGTAAT791340.32667401443080624No Hit
CGTGAA783890.3235985709962402No Hit
TGACAC783340.32337152483664133No Hit
ATTCGA781510.3226160803419761No Hit
TCCAGA778940.3215551555598506No Hit
TACTCG774790.3198419890828776No Hit
AGAGGT751630.31028128170777025No Hit
TTCTAC748460.3089726702060824No Hit
GCGTAT747740.30867544614260756No Hit
CCGACT743470.306912742321722No Hit
GACTAA735450.3036019965035717No Hit
AATTGC730680.30163288708305086No Hit
CTCGTC723540.2986854151202587No Hit
GCGGCT720480.29742221285049064No Hit
ACGCTA718730.29669979325176704No Hit
AGGTGT717940.29637367240434326No Hit
GGCGTT694000.2864909722938048No Hit
TCTCGC692390.28582634626297915No Hit
CTGAGG675670.2789241430111745No Hit
AAGGCG665970.27491987437824955No Hit
CGCTTG659450.27222834535900514No Hit
GCCTGG647090.2671259989360204No Hit
AAGCGC641860.26496699636383514No Hit
GGTGGT633290.26142920438608597No Hit
TCATGT625600.25825468626369497No Hit
GTTGGC624660.25786664373638063No Hit
TCTCTG617440.25488614687764677No Hit
CATACG602240.24861141664873346No Hit
GACACG591940.24435946129624614No Hit
CGTCTC571060.23573996345547577No Hit
GTCGAG565690.2335231673153926No Hit
CAGTAG554760.2290111409073648No Hit
CCACTT538380.22224929346331218No Hit
AGGACT524740.216618548705261No Hit
GTGGTG507950.20968744866950742No Hit
AACAAT501540.20704132888218282No Hit
ATGCCG489860.20221969407470206No Hit
GGCCAG487880.20140232790014628No Hit
GACCGA471130.1944877403123635No Hit
TTAGCG460480.19009130104013147No Hit
ATGGCC451270.18628930989484913No Hit
ACTAGC436880.18034895673734505No Hit
GCTGTC417120.17219180743975773No Hit
CATTAA416670.17200604240008596No Hit
AATGTA410390.16941358806866652No Hit
TCAATG383210.15819337967249128No Hit
AGACGG378060.15606740199624763No Hit
AAGTTG338150.1395921070333575No Hit
CCTTAT319100.13172805368725232No Hit
CGTTGC315800.13036577672965932No Hit
GCTTCT312520.12901175599605172No Hit
GAGCAC286210.1181506933432419No Hit
TGTGTG261420.10791710371332343No Hit
TTCGTT247170.1020345441237172No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)