FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24813472
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTCG4467271.8003405569361677No Hit
TACGAA4408691.7767324137468548No Hit
AGTGCA4331571.7456525229520479No Hit
CGACAA4245621.7110140813828876No Hit
AGTTAG3287841.3250221492582739No Hit
TTCCAT3150661.2697376650877394No Hit
GCCATA3046161.2276234458442574No Hit
CGAAGC2940581.1850739791674458No Hit
GAACCG2753431.1096512410677555No Hit
TCGGAC2718451.0955540603104637No Hit
CAGTCC2714741.0940589047755993No Hit
TGCGAC2657931.0711640837686882No Hit
CCAGCG2408460.9706259567383395No Hit
ACTTGA2391840.9639279823476538No Hit
CGTACT2391020.9635975167038293No Hit
GATCAG2337610.9420729190981416No Hit
CCATGA2235340.900857405203109No Hit
GACTTG2223290.8960011722664205No Hit
TTCACA2200130.8866675328627933No Hit
ATCAGC2168770.8740292370209216No Hit
AGGTCA2154810.8684032609382516No Hit
CAACGG2097500.8453069364899842No Hit
GTAAGA2056510.8287876843675888No Hit
AGAACG1994860.8039423100483479No Hit
CCGGAA1970970.7943144756203404No Hit
ATGTGA1954670.7877454634321226No Hit
ACAGTG1921220.7742648832053813No Hit
TTAGTA1909910.7697068753619002No Hit
TAGCTT1900310.7658380092878578No Hit
CTTGTA1869350.7533609161990712No Hit
CAGATC1856210.7480654057602257No Hit
GGCTAC1851350.7461067923102418No Hit
CACCTA1834130.7391670137899283No Hit
ACCGCT1782070.7181864754759028No Hit
TCGTGG1773540.7147488267663631No Hit
CCTCGG1771080.7137574298348897No Hit
TGATAA1760770.7096024288741213No Hit
AACCTT1751810.705991487205015No Hit
GAGAAG1736690.6998980231383983No Hit
GTTACA1683340.6783976059456733No Hit
TTACGT1647790.6640707112652353No Hit
AGTAGG1640630.6611851819850121No Hit
GAGATA1614910.6508198449616402No Hit
TTAGGC1610450.6490224342647413No Hit
GGAGGC1600040.6448271326157017No Hit
GAGTCG1580950.6371337312247154No Hit
GCTCAA1578260.636049642710218No Hit
TAAGTC1566080.6311410188787768No Hit
AGGCTT1548210.6239392858847No Hit
AGTCAA1540720.6209207643331817No Hit
ACGTAA1539270.6203364043532481No Hit
TACGGC1522860.6137230614079319No Hit
ACAGCC1498640.6039622347086293No Hit
CTGTAT1496500.6030997999796239No Hit
GCCAAT1490930.600855051642914No Hit
TTCTGT1490270.6005890671003236No Hit
CGAATT1475250.5945359037219781No Hit
TGACCA1470020.5924281777253905No Hit
TATAAG1462330.5893290547973294No Hit
GCGCAG1461580.5890267996352949No Hit
AGATGC1450290.584476851929468No Hit
AAGCAT1446030.5827600426091117No Hit
TCTTCA1445340.5824819678600399No Hit
GACCAT1443520.5817484953335027No Hit
CAGCCT1419030.5718788567758676No Hit
TACAGT1414990.5702507089697081No Hit
GTACGC1413520.5696582888521203No Hit
ATTCAT1404770.5661319786283838No Hit
AATCGG1393630.5616424819549638No Hit
CAAGCT1379640.5560044156658125No Hit
CTTCAC1369090.551752693053193No Hit
CCTGCT1351810.5447887341199168No Hit
ACTAAT1332220.5368938292875741No Hit
CTATGC1319790.5318844537354547No Hit
GCACAT1282800.5169772291439102No Hit
ACCTGC1282330.5167878159090352No Hit
CGAGAG1269220.5115043956766712No Hit
CTACCG1263750.5092999480282324No Hit
CTAGTT1259270.5074944771936792No Hit
AACTAG1259190.5074622366430623No Hit
CGAGCC1257140.5066360725335012No Hit
GTCTGC1254910.5057373671850518No Hit
ACGCTA1253770.5052779393387592No Hit
TGCATA1253220.5050562855532672No Hit
GAGTGA1233500.4971089898261718No Hit
AGTTCC1228460.49507783513729964No Hit
GGACTA1217920.4908301425935072No Hit
CATAAC1214720.48954052056882646No Hit
TCAAGC1210060.4876625084953851No Hit
GCATAA1200010.483612289324122No Hit
TCCACG1194230.48128290954204234No Hit
ATTGAA1192170.480452715363654No Hit
TATTGG1170200.47159865415045504No Hit
TATGGT1154390.46522711533476657No Hit
CTATTA1139870.45937545539777747No Hit
ATACCT1092930.44045831232324123No Hit
TCTCTG1084680.43713350554086106No Hit
ATCCAC1081360.43579552269025473No Hit
TAGAGC1071310.4317453035189916No Hit
CGTGAA1058010.4263853119789121No Hit
GGATTC1055790.4254906366992898No Hit
TATTCT1046080.4215774398681491No Hit
CGTCAG1044950.4211220420906836No Hit
ATCCGG1043920.42070694500148953No Hit
ACGAAC1033810.41663254541726363No Hit
CCGCTC1032780.4162174483280695No Hit
AACTCT1024350.4128201003068011No Hit
CATACG1023640.41253396542007503No Hit
GAGCCA1016600.4096967969657773No Hit
AGAGGT1010230.4071296431228971No Hit
ATTGCT1005180.40509445836519775No Hit
ACACGA1002430.40398618943773773No Hit
CAAGGA973160.39219017798073563No Hit
GGCAGG971170.3913881942841373No Hit
AACATA970340.3910536985714857No Hit
TTGAAT970040.3909327965066719No Hit
CGTTGC958760.38638687886967205No Hit
CACATT943360.38018057287589585No Hit
CTGTTC931080.3752316483561833No Hit
AATTGC913650.3682072383905001No Hit
TAATGA909410.3664984892077981No Hit
CTCGTC873480.35201845191192915No Hit
GGCCGT869750.35051523623941055No Hit
TCCAGA865200.3486815549230676No Hit
GGTACC861180.3470614672545624No Hit
GGATCG860850.3469284749832672No Hit
CAGGAC858020.3457879655051901No Hit
AAGAGG856650.34523584607587365No Hit
GATCCT837710.33760289571729424No Hit
TGTGAT825190.3325572495457306No Hit
GTCTCA813090.3276808662649064No Hit
GTGGTG810080.3264678155479411No Hit
GGTAAT805440.3245978636121539No Hit
GCGTAT787010.317170446763758No Hit
TGGCAT784490.3161548694193219No Hit
GCCTGG767150.3091667300730829No Hit
GTACTT747880.30140078744320825No Hit
ACCAGG742310.2991560391064983No Hit
AGCCTA739150.297882537357126No Hit
CTGGAG732380.29515418076116073No Hit
TTAGCG732070.2950292486275198No Hit
CTCCGC723550.29159562998680716No Hit
GATTCA719350.28990300107941364No Hit
AGGTGT714450.2879282673541212No Hit
GAATAC709280.2858447217704963No Hit
CTTAGC703890.28367251467267457No Hit
GGCGTT689830.2780062379017334No Hit
TTCTAC686930.27683751794186645No Hit
GTCGAG675750.2723319009931379No Hit
CGCTTG667520.269015154348412No Hit
TCCTCC663910.26756029950181903No Hit
TCATGT663900.2675562694329919No Hit
AAGGCG662340.26692757869596No Hit
GACTAA640460.25810978810220514No Hit
AACAAT637510.25692091779820253No Hit
TGACAC635310.2560343026562345No Hit
ATTCGA630010.25389836617785694No Hit
TACTCG616490.2484497131235806No Hit
CCACTT612210.24672484366557007No Hit
CTGAGG589470.23756046715268223No Hit
GACACG579130.23339337598543242No Hit
CGTCTC566480.2282953389191162No Hit
AAGCGC559230.22537353901944882No Hit
TCTCGC555900.22403152610001534No Hit
GCTGTC541460.2182121067136433No Hit
GAGCAC512940.2067183504186758No Hit
CCTGAC505260.2036232575594419No Hit
GTTGGC502750.2026117102838329No Hit
TGCGGA498740.2009956526841548No Hit
CATGAG497560.20052010456255376No Hit
CAGTAG492270.19838819815300332No Hit
GCGGCT460570.18561287997100928No Hit
AATGTA457240.18427086705157586No Hit
GGCCAG444850.17927761177476492No Hit
CCTTAT436850.17605355671306297No Hit
ATGCCG430200.1733735609430232No Hit
ACTAGC371600.14975735761605632No Hit
AAGTTG369290.14882641171698988No Hit
GACCGA368130.1483589237330431No Hit
CCGACT366930.1478753154737878No Hit
TTCGTT366350.1476415714818144No Hit
AGGACT362850.1462310473923198No Hit
GCTTCT352340.14199544505500883No Hit
GGTGGT327590.13202102470786836No Hit
ATGGCC317990.128152158633826No Hit
TCAATG303190.12218765676967737No Hit
TGTGTG292440.11785533278051537No Hit
CATTAA292050.11769816009625739No Hit
AGACGG291550.11749665665490101No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)