FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25265631
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGC3595601.4231190188758793No Hit
ATTGCT3302711.3071947421380452No Hit
CTTAGC3065771.2134151725717834No Hit
AATTGC2777881.099469868771534No Hit
GTCGAG2606231.0315317278242526No Hit
ATTCAT2571041.0176037162895317No Hit
AGAGGT2539351.0050609858111201No Hit
ATTCGA2468850.9771574673911767No Hit
AGGTGT2450810.970017333032371No Hit
TCCAGA2395760.9482288409895641No Hit
GGCCGT2388000.9451574749904327No Hit
ATCAGC2325530.9204321871082499No Hit
TCTCGC2307570.9133237163164458No Hit
ACTTGA2285180.9044618755019418No Hit
CAGTCC2282760.9035040526001508No Hit
AGGTCA2238310.8859109831850231No Hit
ACCAGG2214540.8765029458397458No Hit
TCATGT2187890.8659550200824194No Hit
AAGCGC2187820.8659273144612933No Hit
GCCAAT2183480.8642095659514699No Hit
GGTAAT2145960.8493593530278345No Hit
TGCGAC2123050.8402916990278217No Hit
GATTCA1999030.7912052542839717No Hit
GTACTT1998660.7910588102865904No Hit
GCGTAT1990280.7877420516431987No Hit
TCGGAC1962520.7767547938937286No Hit
CTTCAC1953370.7731332734179487No Hit
AACATA1945620.7700658653646925No Hit
GTGGTG1906560.7546061287762811No Hit
GATCAG1885560.7462944424384255No Hit
GTCTCA1857870.7353348903100817No Hit
TGATAA1845700.7305180701800007No Hit
ACAGTG1845510.7304428692083724No Hit
TACTCG1834690.7261603717714392No Hit
TGTGAT1828860.7238528893262155No Hit
CTGTAT1814060.7179951294309649No Hit
CCAGCG1813690.7178486854335837No Hit
TTCCAT1803850.7139540666924171No Hit
TGACCA1802750.7135186926461484No Hit
CCACTT1795990.710843121234534No Hit
CGTACT1718140.6800305125963408No Hit
TTCTAC1716700.6794605683903164No Hit
ATTGAA1692160.669747769212651No Hit
AGTGCA1657310.6559543278376859No Hit
TGCGGA1615870.6395526001309843No Hit
GACCAT1615380.639358660783101No Hit
TACGAA1602490.6342568685500077No Hit
CTAGTT1602290.6341777096325043No Hit
CATGAG1594400.6310548903369957No Hit
CCGCTC1592320.6302316375949605No Hit
TACAGT1578190.6246390600733464No Hit
AACCTT1560990.6178313931680551No Hit
GTAAGA1559350.6171822900445273No Hit
AGAACG1555760.6157613874753415No Hit
TTCTGT1539080.6091595337555591No Hit
AGTTCC1535980.6079325705342565No Hit
GGCTAC1534530.6073586683823571No Hit
CGACAA1528960.6051540925298877No Hit
CCGTCG1508570.5970838408904175No Hit
TATTGG1503900.5952354801667135No Hit
CCTGAC1497850.592840922912236No Hit
GACTTG1495830.5920414178454518No Hit
GACACG1479000.5853801949375418No Hit
CGTCTC1469500.581620146356131No Hit
TCCTCC1467190.5807058608589668No Hit
GAATAC1464670.5797084584984241No Hit
TATGGT1458480.5772584900016944No Hit
GCTGTC1456200.5763560783421557No Hit
GAGAAG1447630.5729641187271357No Hit
TTCACA1444870.571871725665589No Hit
AGTTAG1444060.5715511320497002No Hit
CTTGTA1443850.5714680151863217No Hit
TAGCTT1430150.5660456293373397No Hit
TATAAG1407060.5569067323115737No Hit
CTGAGG1403730.5555887363351424No Hit
TGACAC1402870.5552483529898778No Hit
TAATGA1384470.5479657325795663No Hit
GCTCAA1383870.5477282558270562No Hit
CAGATC1378410.5455672173792137No Hit
CAAGGA1368020.5414549116149128No Hit
GTCTGC1366330.5407860187620092No Hit
TAAGTC1364180.5399350603988478No Hit
CTACCG1358140.5375444610902455No Hit
CCGGAA1352200.5351934412403949No Hit
AGTAGG1342610.5313977711461075No Hit
TAGAGC1333930.5279622741264606No Hit
ATGTGA1332290.5273131710029328No Hit
GTTACA1330010.5264107593433942No Hit
GCCATA1328970.5259991329723765No Hit
GGATTC1322430.5234106363700158No Hit
ACCGCT1321740.5231375381046292No Hit
TCCACG1303100.5157599269933135No Hit
ACTAAT1295370.5127004348318077No Hit
GCGCAG1285030.5086079187968826No Hit
ACGCTA1261790.4994096525829891No Hit
CGAAGC1257140.49756920775103536No Hit
TATTCT1250230.4948342671512934No Hit
ATACCT1242630.4918262282861647No Hit
ACAGCC1230560.4870489876148353No Hit
TTGAAT1199620.47480310307706153No Hit
TCTTCA1198710.4744429300024211No Hit
CCTCGG1192150.47184651750830997No Hit
GTTGGC1180770.4673423751023673No Hit
TTAGGC1174480.46485282714688586No Hit
AACTCT1161180.45958875913291064No Hit
GACTAA1148760.4546729903559503No Hit
GAGTCG1145650.45344206918877267No Hit
CAACGG1141000.45160162435681894No Hit
AAGCAT1135770.4495316186641054No Hit
GAACCG1122900.4444377423227625No Hit
CGAGCC1122510.44428338243363086No Hit
GCGGCT1114930.44128325946025254No Hit
ACGTAA1110780.4396407119220572No Hit
ACACGA1091700.43208895119223417No Hit
GGCAGG1091130.4318633482773495No Hit
ATCCAC1079170.4271296450106471No Hit
CTGGAG1067660.42257404930832715No Hit
GGACTA1050410.41574659267366015No Hit
GGAGGC1042810.4127385538085314No Hit
TTACGT1040740.41191925901237136No Hit
TACGGC1032300.4085787526937285No Hit
AACTAG1021470.4042922973109201No Hit
GTACGC1016980.4025151796129691No Hit
CCGACT1005460.397955625964774No Hit
GAGATA1002640.39683948522797635No Hit
GGATCG950860.37634524148634957No Hit
AGTCAA950110.37604839554571184No Hit
CTGTTC949800.37592569922358166No Hit
ACGAAC947220.3749045491877879No Hit
ATCCGG942120.3728859967914516No Hit
CTATGC939500.3718490149721572No Hit
AATCGG938170.37132260817075974No Hit
AGCCTA919540.36394895500531926No Hit
GAGTGA904640.35805161565131705No Hit
CAGCCT886060.3506977522152524No Hit
GGTACC881960.34907499440643297No Hit
AAGGCG877160.34717518038635176No Hit
AACAAT875870.3466646053684549No Hit
TCAAGC856550.3390178539376278No Hit
ATGCCG853850.33794920855133204No Hit
CAGGAC852250.337315937211305No Hit
CACATT842810.3335796363051451No Hit
CAGTAG837420.33144630347842885No Hit
TTAGCG825010.32653449264734374No Hit
CGAATT823250.32583789417331394No Hit
GGCCAG813060.32180474732651637No Hit
CATACG808320.31992868098168614No Hit
GAAGAT802960.31780722199259537No Hit
GCATAA795850.31499312247535No Hit
CGTCAG794310.3143835988105739No Hit
GAGCCA786080.3111262093553096No Hit
TGGCAT773480.3061391975525963No Hit
CGTGAA759140.30046350316760345No Hit
GGCGTT752660.2978987542404937No Hit
CTCGTC752210.2977206466761111No Hit
TGCATA684250.27082244650846043No Hit
ATGGCC651660.25792350090128363No Hit
GATCCT649640.25712399583449946No Hit
TCTCTG640070.2533362416319624No Hit
CCTTAT559190.22132437539359298No Hit
AGACGG550250.2177859717811916No Hit
CAAGCT537260.21264460008934669No Hit
AGGCTT529240.2094703274974609No Hit
GCACAT487930.19312005308713642No Hit
TCGTGG472870.18715938659913145No Hit
GGTGGT460750.18236235619842625No Hit
CACCTA460320.18219216452579395No Hit
TTAGTA455470.18027256077633683No Hit
CCTGCT448300.17743471358384044No Hit
ACTAGC443510.17553885750963433No Hit
CCATGA439330.17388443613381357No Hit
GACCGA379740.1502990366636796No Hit
CATAAC358100.14173404178981322No Hit
AGATGC350810.13884869924681478No Hit
GAGCAC317910.1258270573175077No Hit
ACCTGC314440.12445365009882398No Hit
AAGTTG308100.12194431241396661No Hit
AGGACT305050.12073713892204No Hit
GCTTCT293670.1162329965160973No Hit
TCAATG273030.10806379622974785No Hit
CATTAA272890.10800838498749546No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)