FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001729981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32549712
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGC11437083.5137269417314663No Hit
TACTCG7472712.295783753785594No Hit
CTCCGC6358421.9534489275972702No Hit
AGGTGT6041531.8560932274915367No Hit
AGAGGT4820541.4809777733210052No Hit
GGCCGT4398651.3513637232796407No Hit
GTCTCA4047161.2433781288141659No Hit
CTTAGC4026231.2369479643936634No Hit
ATTCGA3996931.2279463486497209No Hit
TAGAGC3993801.2269847425992586No Hit
GTGGTG3624221.113441495273445No Hit
TCATGT3548591.0902062666483807No Hit
GGTAAT3455331.061554707457934No Hit
TCCAGA3357991.0316496809557025No Hit
TTCTAC3178940.9766415137559434No Hit
GTCGAG3101020.9527027458799022No Hit
AATTGC3029650.9307762845950834No Hit
ATTGCT2969460.9122845695224584No Hit
AAGCGC2945570.9049450268561515No Hit
TGTGAT2938560.9027913979699728No Hit
ACTTGA2927900.8995164073955555No Hit
GGCTAC2603180.7997551560517648No Hit
TGCGAC2488770.7646058435171408No Hit
CTTGTA2470050.7588546405571883No Hit
GATTCA2456030.7545473827848308No Hit
CCACTT2436120.7484305851922746No Hit
CTGAGG2430850.7468115232478861No Hit
ACTAAT2400130.7373736517238616No Hit
TGACCA2388080.7336716220407726No Hit
TGATAA2386230.7331032606371448No Hit
TTCACA2375990.72995730346247No Hit
AGTGCA2346620.7209341821519035No Hit
GATCAG2337990.718282853009575No Hit
ACCAGG2313540.710771265810278No Hit
CAGATC2293840.7047189849175931No Hit
GCCAAT2276850.6994992766756277No Hit
GAGAAG2272380.6981259926355109No Hit
ACCGCT2250730.6914746281011641No Hit
GGACTA2228580.6846696523766478No Hit
TAGCTT2177950.6691149832600669No Hit
GTACTT2091820.6426539196414396No Hit
AGGTCA2090070.6421162804758457No Hit
CAGGAC2075970.637784444913061No Hit
CTTCAC2057970.6322544420669528No Hit
TGCGGA2023790.6217535811069542No Hit
ATCAGC1983830.6094769747885942No Hit
CGAAGC1925590.5915843433576309No Hit
CGTGAA1919310.5896549868090999No Hit
CATACG1912900.5876856913511247No Hit
ACAGTG1910790.5870374521286087No Hit
TACAGT1891390.5810773379500255No Hit
AGTTCC1867640.5737808064169662No Hit
AACCTT1864740.572889861513982No Hit
CTAGTT1848890.5680203867856035No Hit
GGAGGC1846810.5673813642344977No Hit
CCGGAA1841290.5656854966950244No Hit
ATTGAA1810100.5561032306522405No Hit
ATTCAT1785310.5484871878436283No Hit
GTAAGA1783700.5479925598112819No Hit
CGTACT1776860.5458911587297608No Hit
ATCCAC1772320.5444963691230202No Hit
GCGCAG1763620.5418235344140679No Hit
TCAAGC1755280.5392612997620378No Hit
GAAGAT1749000.5373319432135067No Hit
CGTCTC1711040.5256697816558255No Hit
CAGCCT1695510.5208986180891555No Hit
CAAGGA1692950.5201121287954867No Hit
TCTTCA1691860.5197772564009168No Hit
AGTCAA1680960.516428532455218No Hit
CCGCTC1673590.5141643035121171No Hit
TTACGT1656530.508923089703528No Hit
AACATA1655640.5086496617850259No Hit
GCGTAT1650830.5071719221355937No Hit
CAGTCC1646070.5057095436051784No Hit
TAAGTC1644210.5051381099777472No Hit
GAGATA1637300.5030152033296025No Hit
GCCATA1629660.5006680243438099No Hit
GGCAGG1625830.4994913626271102No Hit
GTTGGC1613210.49561421618722773No Hit
GTACGC1612100.49527319934505104No Hit
GACTAA1600500.49170941973311466No Hit
AGAACG1591060.48880924046271135No Hit
ATACCT1589310.4882716012971175No Hit
CGAGCC1578150.48484299953253046No Hit
CCAGCG1575920.4841578936243737No Hit
GAACCG1570800.4825849150370363No Hit
CACATT1566800.48135602551567896No Hit
GACACG1565470.4809474197498276No Hit
TATTCT1541360.47354028815984606No Hit
GACCAT1531860.4706216755466224No Hit
TATTGG1530780.47028987537585587No Hit
GGATTC1512270.4646031891157747No Hit
TATAAG1510130.4639457332218485No Hit
AATCGG1502980.4617490932024222No Hit
CGAATT1493040.45869530274184916No Hit
TCCACG1469630.4515032268181052No Hit
GTCTGC1463120.44950320912209607No Hit
TCCTCC1450280.44555847375853896No Hit
CATGAG1432860.44020665989302765No Hit
AACTCT1421840.43682106926168807No Hit
TTCTGT1405450.4317856944479263No Hit
TTGAAT1401690.43063053829785036No Hit
CTGTTC1399480.42995157683730045No Hit
TTAGGC1393430.4280928814362474No Hit
ATGTGA1391370.4274600033327484No Hit
TGGCAT1374290.42221264507655243No Hit
AAGGCG1355120.4163231920454473No Hit
CTGTAT1344500.41306049036624354No Hit
TCGGAC1326680.4075857875485965No Hit
GCTCAA1326280.40746289859646073No Hit
ACGCTA1318270.4050020473299426No Hit
CCTCGG1317620.404802352782722No Hit
AAGCAT1314310.4037854467037988No Hit
GATCCT1304440.4007531618098495No Hit
CCGTCG1292170.39698354320308576No Hit
GACTTG1284960.3947684698408392No Hit
TGCATA1280210.39330916353422724No Hit
TTCCAT1271460.39062096770625804No Hit
ACGAAC1257980.3864796100192837No Hit
TGACAC1244340.3822890967514551No Hit
CTGGAG1238410.38046726803604286No Hit
TAATGA1235110.37945343418092303No Hit
CTCGTC1225430.3764795215392382No Hit
GAGTCG1217670.3740954758678049No Hit
AGTTAG1216910.373861986858747No Hit
GAGCCA1206320.3706085018509534No Hit
TACGAA1187810.3649218155908722No Hit
GGTACC1187200.3647344099388652No Hit
CTATGC1185720.364279720815963No Hit
TTAGCG1171510.35991409079134096No Hit
CGTCAG1161490.35683572254034074No Hit
CGACAA1159420.35619977221303833No Hit
ATCCGG1139630.3501198413061228No Hit
CTACCG1137310.3494070853837355No Hit
GCGGCT1118440.34360979906673217No Hit
GCTGTC1117650.3433670933862641No Hit
AACTAG1113230.34200917046516416No Hit
ACACGA1090460.3350137168648374No Hit
TACGGC1069910.32870029694886393No Hit
CCTGAC1047630.32185538231490346No Hit
GGCGTT1044100.3207708873123056No Hit
GTTACA1043420.3205619760936748No Hit
GGATCG1036580.3184605750121537No Hit
TCTCTG1031530.31690910199144007No Hit
AGCCTA985260.30269392245313875No Hit
GGCCAG968350.2974987920016005No Hit
ACGTAA967540.29724994187352566No Hit
TATGGT958900.2945955405073937No Hit
AGTAGG958570.29449415712188176No Hit
CCTGCT952660.2926784728540762No Hit
ATGGCC887870.2727735348318904No Hit
ACAGCC880440.2704908725459691No Hit
AACAAT874380.2686291049211127No Hit
CCTTAT862120.2648625585381524No Hit
CAACGG802100.24642307127018512No Hit
CAGTAG788390.2422110524357328No Hit
GAATAC768060.23596522144343396No Hit
CCGACT766450.23547059341108761No Hit
GAGTGA697260.21421387691540864No Hit
NNNNNN658840.20241039306277117No Hit
GCATAA595480.18294478304447057No Hit
ATGCCG590590.1814424656046112No Hit
CACCTA506370.1555681967324319No Hit
CCATGA502870.1544929184012442No Hit
GGTGGT477120.14658194210750622No Hit
CAAGCT456970.1403914111436685No Hit
AGGCTT403820.12406254162863253No Hit
TTAGTA392380.12054791759755047No Hit
CATAAC373320.11469225902828264No Hit
GCACAT360700.11081511258840017No Hit
ACCTGC343310.10547251539429904No Hit
AGACGG341900.10503933183802056No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)