FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27203578
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC5555172.0420732890357294No Hit
AAGCAT4585141.6854915187994757No Hit
TCAAGC4290621.5772263486810447No Hit
GAAGAT4279931.5732967185419506No Hit
TAAGTC4264291.5675474748211429No Hit
TACGGC3858151.4182509374318335No Hit
CCTCGG3816051.402775032019685No Hit
ATCCGG3230831.1876489188297215No Hit
ACGAAC3180851.169276335634967No Hit
AACCTT3140051.154278308537208No Hit
CGAGCC3118821.146474188064526No Hit
CTGTTC3085011.1340456759033684No Hit
TGCATA2845841.046127094016824No Hit
CTTGTA2774871.0200386140381974No Hit
CAGGAC2739191.0069226923017258No Hit
AACTAG2737291.0062242547653106No Hit
CTATGC2716180.998464246137034No Hit
ACAGCC2669160.9811797551042735No Hit
CCGACT2638620.9699532907031568No Hit
TCCACG2631290.9672587922074075No Hit
ACACGA2490650.9155597105645442No Hit
ACTTGA2478160.9109684027593722No Hit
GCATAA2470860.9082849322247243No Hit
CATACG2451590.9012013052106601No Hit
AGTAGG2445630.8990104169385366No Hit
TGGCAT2434110.8947756798756399No Hit
AGGACT2430770.8935479002063625No Hit
CGTTGC2422180.8903902273443589No Hit
ACGTAA2415500.8879346680058042No Hit
GTAAGA2380710.8751459091153376No Hit
CCTTAT2348900.863452594360933No Hit
ATGGCC2330760.8567843538816842No Hit
AGTGCA2277350.8371509071343483No Hit
GAATAC2246190.8256965315371382No Hit
TAGCTT2183750.802743668498313No Hit
TAATGA2135140.7848746955271839No Hit
GAGAAG2102430.7728505419397405No Hit
AGCCTA2035540.74826186467089No Hit
GTTACA2025130.7444351621687412No Hit
GCCATA1962160.7212874718171264No Hit
CATTAA1960830.7207985655416358No Hit
CAGTCC1960560.720699313891724No Hit
TCGGAC1957010.7193943384947378No Hit
TGCGAC1942530.7140715092698467No Hit
AGGTCA1879340.6908429472034892No Hit
GACCGA1865040.685586285745206No Hit
ACTAGC1861580.6843143942315235No Hit
CAGTAG1856840.6825719763775192No Hit
AACAAT1853500.6813441967082419No Hit
GTGGTG1816660.6678018604758535No Hit
GAGTGA1799850.6616225262720955No Hit
GGCTAC1796760.6604866462786624No Hit
AGACGG1769840.6505908891837684No Hit
CGAATT1747450.6423603542151698No Hit
CCAGCG1728850.6355230183323679No Hit
CAACGG1717830.6314720806211594No Hit
CAGCCT1700880.6252412825989287No Hit
GGCCAG1697840.6241237825406644No Hit
CGTACT1684300.6191464960969473No Hit
GAGTCG1662150.6110041848171589No Hit
ATGCCG1653170.6077031484608385No Hit
CAGATC1645540.6048983703540762No Hit
GGCGTT1626230.5978000393918771No Hit
AATGTA1618530.5949695293758784No Hit
GGTGGT1606770.5906465686241714No Hit
ATGTGA1525800.5608821016117806No Hit
TCAATG1494440.5493542062738953No Hit
TATTCT1481860.5447298145854197No Hit
TCATGT1457310.5357052664175278No Hit
TTCACA1417930.5212292294785634No Hit
GGACTA1408470.5177517457446222No Hit
CGAAGC1403240.5158292045259635No Hit
GAGATA1383690.508642649874954No Hit
TTCCAT1382130.5080691958976866No Hit
ATTCGA1362950.5010186527669265No Hit
GGAGGC1357690.49908508358716636No Hit
TGTGTG1347150.49521059325357863No Hit
GAACCG1343980.4940453053638753No Hit
AAGTTG1342370.4934534714514392No Hit
GCTCAA1333970.49036564234307706No Hit
CTGAGG1315090.4834253788233298No Hit
TTCGTT1313610.4828813327423327No Hit
GAGCAC1300190.4779481581430207No Hit
CAAGCT1287670.4733458223767476No Hit
TATGGT1279450.47032416103499325No Hit
GATCAG1274130.46836853593303057No Hit
CCATGA1268370.46625116740158223No Hit
CACCTA1260630.4634059534374486No Hit
ATCAGC1259340.462931751110093No Hit
AATTGC1254110.4610092098914342No Hit
AATCGG1220580.4486836253672219No Hit
TTGAAT1220360.4486027536524791No Hit
AGAACG1214710.44652582097840215No Hit
TCTCTG1201790.44177644573077846No Hit
CTACCG1197590.44023253117659744No Hit
GCGCAG1185520.4357956148268437No Hit
GCCAAT1184490.435416988162366No Hit
AGGCTT1177310.4327776294721231No Hit
AAGGCG1168140.4294067493621611No Hit
TTAGTA1167310.42910164243835863No Hit
ACAGTG1161840.4270908775308895No Hit
GACTTG1134820.4171583605656579No Hit
GCTTCT1131450.4159195529352793No Hit
CCTGCT1126910.4142506548219503No Hit
CCACTT1125260.4136441169613791No Hit
TGATAA1106650.4068031050915435No Hit
CGACAA1099810.40428872996044857No Hit
TGACCA1098270.4037226279572489No Hit
TTCTAC1096040.40290288284871945No Hit
TCTTCA1088980.4003076360028817No Hit
TTACGT1072440.39422755344903526No Hit
TACGAA1070610.39355484782185635No Hit
GTACGC1044920.3841112371321155No Hit
CACATT1017970.3742044520761203No Hit
CCGCTC1017570.3740574125947697No Hit
GGCCGT1017140.3738993451523178No Hit
CTTAGC1009580.3711202989547919No Hit
TGTGAT982110.36102236257304093No Hit
CGTGAA956840.35173314333871813No Hit
ATCCAC950420.3493731596630414No Hit
TCCTCC922730.3391943515665476No Hit
CATAAC919390.33796657189727025No Hit
CCGTCG905610.3329010617647429No Hit
GTCGAG898620.33033154682814153No Hit
TACAGT892170.3279605351913634No Hit
CTTCAC870870.3201306828094451No Hit
ATTGAA863860.31755381589877624No Hit
TCGTGG852320.3133117268618121No Hit
GGATTC850970.3128154686122539No Hit
GATTCA845930.3109627711472366No Hit
CTGGAG828460.30454082179925007No Hit
TATAAG825060.3032909862077702No Hit
ACGCTA824320.30301896316727156No Hit
TACTCG815570.2998024745127277No Hit
CCGGAA811290.2982291520622765No Hit
CTCCGC783800.28812386370645804No Hit
ATTCAT772070.28381193091585233No Hit
CGTCAG750480.27587547490995484No Hit
CTAGTT749150.2753865686344642No Hit
GGTAAT741810.2726883941516811No Hit
ATACCT726800.2671707376140006No Hit
GCGTAT717440.26373001375039706No Hit
AAGCGC704940.25913502995819154No Hit
CTGTAT698300.25669417456777194No Hit
GTTGGC693200.25481942118055206No Hit
TCCAGA669340.24604851611799006No Hit
AACATA656270.24124400106485994No Hit
GCTGTC654680.24065951912649136No Hit
AGATGC654460.2405786474117486No Hit
CATGAG652880.23999784146041375No Hit
TCTCGC649880.23889504535028447No Hit
TTCTGT637720.2344250451172269No Hit
AGTCAA634980.2334178246699754No Hit
AGAGGT634350.23318623748684822No Hit
ACCAGG621890.2286059576427777No Hit
TGCGGA620990.22827511880973891No Hit
AGGTGT601810.22122457567897869No Hit
GTCTGC600480.22073566940348802No Hit
GTCTCA598980.2201842713484234No Hit
ACCGCT575110.21140969029882759No Hit
GCACAT556420.20453927053272186No Hit
GACACG550190.20224913061068658No Hit
CGTCTC520460.19132042115930487No Hit
AACTCT511420.18799732888078177No Hit
GGCAGG502650.18477348825217038No Hit
GACTAA499990.18379567570118902No Hit
TGACAC493160.18128497655712789No Hit
CTCGTC493120.1812702726089928No Hit
ACCTGC474170.17430427718000918No Hit
TAGAGC467180.17173476224340783No Hit
AGTTCC457680.1682425745613316No Hit
GTACTT435850.1602178948666238No Hit
CAAGGA398810.14660203889356024No Hit
GCGGCT396940.1459146293182463No Hit
TTAGCG396300.14567936614808535No Hit
AAGAGG383050.14080868332834748No Hit
ACTAAT363420.13359272078106785No Hit
CCTGAC360780.13262226020415402No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)