FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21789358
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATAA5567772.5552703296719437No Hit
AACTAG4406852.0224781290022404No Hit
CCTCGG3934491.8056934031741552No Hit
TGCGAC3892821.786569388597865No Hit
TACGGC3813621.7502213695327784No Hit
ACGTAA3662651.6809352528881298No Hit
GAGTCG3301901.5153727796844678No Hit
GCATAA3228131.481516802835586No Hit
CAGTCC3071521.4096422666514543No Hit
ACAGCC2989981.3722203288412627No Hit
AGTAGG2858281.3117779789565163No Hit
TCGGAC2856261.3108509208945027No Hit
ATTCAT2764061.2685366865788335No Hit
CGTACT2759141.2662787035763055No Hit
CGACAA2752851.2633919732742929No Hit
CGAAGC2751421.2627356895967288No Hit
TTCCAT2728661.252290223511863No Hit
TCTTCA2672761.2266354979343586No Hit
GTTACA2607591.1967264019435544No Hit
AGTGCA2537061.1643573895109713No Hit
TTCACA2528431.160396740463854No Hit
GAGTGA2444271.1217723808108526No Hit
GCCAAT2425531.1131718520573208No Hit
CTTGTA2420291.1107670083717014No Hit
ACTAAT2411651.1068017699282375No Hit
CCGGAA2310121.0602056288211887No Hit
CCAGCG2269941.0417654343005425No Hit
ACAGTG2205351.012122523297841No Hit
TTCTGT2187041.0037193385872132No Hit
TATGGT2184941.0027555653544267No Hit
ATGTGA2175260.9983130296909161No Hit
GCCATA2159080.9908873864021143No Hit
AGAACG2149310.9864035461714842No Hit
GCTCAA2143710.9838334842173873No Hit
GGCTAC2129610.9773624353686786No Hit
AGTTAG2127260.9762839272272271No Hit
GACTTG2105270.9661918446610497No Hit
ATTGAA2102240.9648012575680293No Hit
CTAGTT2100850.9641633314758516No Hit
TACGAA2089890.959133353079976No Hit
GATCAG2080870.9549937175753411No Hit
CAACGG2064530.9474946439449936No Hit
CTGTAT1953300.8964467883817412No Hit
ATCAGC1925340.8836148361966424No Hit
CGAGAG1821830.8361099946129666No Hit
CCGTCG1778730.8163296963591126No Hit
CAGATC1681890.7718859821386201No Hit
TAGCTT1677100.7696876612885978No Hit
GCGCAG1586830.7282591804678228No Hit
CATTAA1559680.7157989693867989No Hit
GAACCG1555620.7139356744700784No Hit
CTACCG1481590.679960373316185No Hit
ACTTGA1459860.669987615055019No Hit
CTATTA1453500.6670687589785802No Hit
TGACCA1414420.6491333980560603No Hit
GACCAT1384810.6355441954737721No Hit
GTCTGC1380560.6335937020264663No Hit
ATACCT1323010.6071817260517727No Hit
TATAAG1253780.575409335144248No Hit
TTAGTA1175580.5395202557138215No Hit
TGTGTG1158320.5315989576195865No Hit
CACCTA1147780.5267617338702683No Hit
ACTAGC1088270.4994502362116406No Hit
AATGTA1078100.4947828201271464No Hit
AGGTCA1074150.49297000857023876No Hit
AGATGC1073580.49270841297848245No Hit
GAGCAC1071000.4915243487210592No Hit
CCTGCT1018690.46751721643198485No Hit
CCATGA1000150.459008475605385No Hit
GCACAT952240.4370206777088155No Hit
AGGCTT917330.4209990950628284No Hit
AGGACT910050.41765801452250223No Hit
TCAATG908390.4168961747289663No Hit
GACCGA907880.41666211551528964No Hit
TACTCG907850.41664834732624983No Hit
TCGTGG876730.40236614589562486No Hit
GAAGAT867590.39817143763483076No Hit
GATCCT839600.3853257172606921No Hit
GCTTCT839130.3851100156324018No Hit
GGTACC776120.3561922292524635No Hit
GACACG770560.3536405248837529No Hit
CGTTGC767850.35239679847382377No Hit
TATTGG762610.34999195478820444No Hit
TAGAGC754320.34618734521687144No Hit
ACCTGC748240.3433969922381376No Hit
ACCAGG740920.340037554112425No Hit
TTCGTT740510.33984938886221433No Hit
GTTGGC722900.33176746189584844No Hit
AACAAT716590.32887155280114266No Hit
TTAGGC715030.32815560697107277No Hit
TCCTCC708260.3250485856444233No Hit
GCGTAT686130.3148922515293934No Hit
GAGCCA683390.3136347569304245No Hit
ACACGA676990.31069754326859933No Hit
CCGCTC667440.3063146697575945No Hit
TCCACG651330.2989211522432189No Hit
CTATGC642060.29466678182991896No Hit
CGAGCC624660.2866812321868317No Hit
CTTCAC615180.28233048445025316No Hit
GCTGTC613040.2813483536320804No Hit
CAAGGA606590.2783881929885222No Hit
AACATA604990.2776538895730659No Hit
GTACTT598770.27479928504547957No Hit
CAGTAG591810.2716050651882446No Hit
AACCTT583330.26771325708632626No Hit
GGCGTT582020.26711204616492146No Hit
AGCCTA576720.26467966610122245No Hit
TCTCGC571500.26228400120829626No Hit
TCAAGC567040.26023713043771185No Hit
CATGAG559840.25693276506815854No Hit
GAATAC558220.256189282860009No Hit
AACTCT557740.25596899183537214No Hit
AAGTTG551480.2530960297223993No Hit
TGCGGA545420.2503148555363586No Hit
AAGAGG544790.2500257235665227No Hit
GTCTCA528530.2425633651069481No Hit
CCGACT527250.24197592237458304No Hit
CTTAGC519860.23858435847444429No Hit
GGTGGT515900.23676695752118992No Hit
GACTAA510480.23427950470133171No Hit
CCTGAC506380.23239785219922499No Hit
AGGTGT505100.23181040946685993No Hit
CCTTAT497250.22820773333477748No Hit
CTGTTC493430.22645458393037557No Hit
GGTAAT491180.22542196975239015No Hit
TGGCAT488270.22408645541552896No Hit
TAAGTC484220.22222774989515523No Hit
AGAGGT477630.2192033377027446No Hit
TACAGT473600.21735381097506404No Hit
GTAAGA470270.21582554199164566No Hit
GAGAAG463120.21254412360382532No Hit
GGCAGG458040.21021271025975155No Hit
ATGCCG454920.20878081859961178No Hit
GGCCAG454670.20866608369094677No Hit
AAGCAT450180.2066054447313225No Hit
GGATCG448780.20596292924279824No Hit
TAATGA441120.20244745164130123No Hit
GGCCGT433730.19905588774116245No Hit
AGACGG430670.19765153245910227No Hit
GGAGGC425580.19531552971868194No Hit
ATGGCC422350.19383315469872953No Hit
ATCCAC417620.19166237022678687No Hit
TGCATA416800.19128603972636551No Hit
CAGCCT415410.19064811363418785No Hit
ACCGCT406270.18645340537339375No Hit
ACGAAC404930.18583842626294908No Hit
GATTCA399560.1833739204248239No Hit
GTCGAG397700.18252029270435594No Hit
ATTCGA395920.1817033801546608No Hit
GGACTA385920.17711398380805896No Hit
GCGGCT381500.17508547062286095No Hit
TGACAC380890.17480551744571823No Hit
AAGCGC372630.1710146760634251No Hit
GAGATA367570.16869244151204454No Hit
TTACGT367100.16847673988375425No Hit
GTGGTG365170.1675909863888601No Hit
ATCCGG362390.1663151342045048No Hit
CTGGAG359290.1648924213370582No Hit
TATTCT345450.15854069679336122No Hit
CTGAGG326220.14971528761884587No Hit
CATACG321890.14772807900076726No Hit
TCCAGA314110.144157528643111No Hit
GTACGC303670.13936619885725868No Hit
CGTCTC294170.1350062723279869No Hit
GGATTC292880.13441424019927525No Hit
ATTGCT290640.13338621541763646No Hit
AGTCAA286950.13169272816574037No Hit
CAGGAC272730.1251666065608725No Hit
TTCTAC267100.12258277641773567No Hit
TGTGAT263570.1209627195073852No Hit
CGTCAG259630.11915449734682408No Hit
ACGCTA259510.11909942459066485No Hit
CTCCGC257940.11837888936424837No Hit
CACATT252460.11586390016631054No Hit
TTGAAT245690.11275687883966108No Hit
CGCTTG238370.10939744071394852No Hit
CGAATT234140.10745612605933594No Hit
GCCTGG228770.10499162022121074No Hit
AATCGG223760.10269233265156322No Hit
AATTGC223640.10263725989540398No Hit
TCTCTG218940.1004802436125011No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)