FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25658395
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATAA8275223.225151066541769No Hit
ATTGAA7178072.7975522241356092No Hit
AGTGCA5810562.2645843592321344No Hit
ATCAGC5441152.1206119868370568No Hit
GACCAT5297902.064782306141908No Hit
TTCTGT4726411.8420520847075585No Hit
ACTAAT4718951.8391446542155112No Hit
ATACCT4670411.8202268692176578No Hit
CAGTCC4606771.7954240707573486No Hit
CTAGTT4084341.5918142970361162No Hit
CTGTAT3822291.489683980623106No Hit
GTCTGC3615561.409113859226191No Hit
TATAAG3575951.3936764166269948No Hit
TACGAA3460871.3488255987952482No Hit
ATTCAT3370951.3137805384943213No Hit
TCGGAC3001181.1696678611425226No Hit
CCGTCG2990741.1655990173976198No Hit
CGACAA2921341.1385513396297782No Hit
CGAAGC2777631.0825423803788194No Hit
AGTTAG2750171.07184023006895No Hit
TTCCAT2708781.055709057405968No Hit
CGTACT2425960.9454839244621498No Hit
GAACCG2359790.9196950939448864No Hit
ACTTGA2282970.8897555751246327No Hit
GGCTAC2265730.8830365266416703No Hit
TAGCTT2249460.8766955220698723No Hit
GCCATA2229660.8689787494502287No Hit
GACTTG2112990.8235082513929651No Hit
GAGATA2031080.7915849763790759No Hit
CAGATC2026230.7896947568232542No Hit
ATTCGA1877620.7317760912169292No Hit
GCCAAT1847610.7200801141302875No Hit
ATCCAC1824060.7109018315448024No Hit
TGCGAC1785040.695694333180232No Hit
GCGCAG1776680.6924361402963825No Hit
CTTGTA1729050.6738730150502399No Hit
TTCACA1614470.6292170652139387No Hit
GTAAGA1601440.6241388052526278No Hit
AGGCTT1551010.6044844192319901No Hit
ATTGCT1522550.5933925329312297No Hit
ACCGCT1511780.5891950763093327No Hit
CCTGCT1510660.5887585719995346No Hit
TATTCT1482180.5776588909789564No Hit
GGATTC1478240.5761233311748455No Hit
AGTCAA1472850.5740226541839425No Hit
CCGGAA1467710.5720194111907624No Hit
CACCTA1451980.5658888640540455No Hit
CTGAGG1446520.5637609055437801No Hit
AACCTT1436970.5600389268307703No Hit
CCAGCG1421710.5540915556097721No Hit
TCGTGG1417100.5522948726917642No Hit
GATCAG1415900.5518271895026949No Hit
AGAACG1387580.5407898662406593No Hit
AGGTCA1383000.5390048754023781No Hit
TTAGTA1381820.5385449869331266No Hit
ACAGTG1365920.5323481846779582No Hit
TCTTCA1331500.5189334718714869No Hit
CATAAC1326180.5168600763999464No Hit
TCCTCC1311030.5109555761379463No Hit
TGACCA1294490.5045093428486076No Hit
AGATGC1286430.5013680707620255No Hit
TTAGGC1281600.49948564592602146No Hit
CTGGAG1268600.49441907804443735No Hit
GCACAT1261020.49146487923348287No Hit
CCACTT1260530.49127390859794623No Hit
ACCTGC1247530.486207340716362No Hit
TCATGT1241560.48388061685074224No Hit
TGTGAT1225580.4776526357163026No Hit
GGACTA1221840.47619502311037No Hit
CAAGCT1205370.4697760713403936No Hit
AATCGG1197080.46654515997590656No Hit
CCATGA1162700.4531460366090708No Hit
TTCTAC1119240.43620811044494406No Hit
ATGTGA1109970.43259525780938357No Hit
AAGAGG1083930.4224465326065796No Hit
GTGGTG1053310.41051281656549443No Hit
AACAAT1052070.4100295439367895No Hit
ACGAAC1050660.40948001618963303No Hit
CCGCTC1029900.40138909701873404No Hit
GACTAA1009600.3934774564036449No Hit
GAGAAG1009370.39338781712574No Hit
CTGTTC1001510.39032449223733595No Hit
AATTGC997610.3888045218728607No Hit
CGAATT987390.38482142004595377No Hit
GCGTAT986680.3845447074924211No Hit
TCAAGC983710.3833871915994746No Hit
TAAGTC978160.381224156850029No Hit
GCTCAA975990.38037842974979535No Hit
TGGCAT960580.37437259813016366No Hit
GCTGTC955520.3724005340162547No Hit
CATTAA947940.36944633520530024No Hit
GGCCGT944920.36826933251280913No Hit
TACTCG931340.36297671775650814No Hit
GACACG926260.3609968589227814No Hit
ATCCGG913130.35587962536238144No Hit
GATTCA911440.3552209715377755No Hit
CTACCG910340.3547922619477953No Hit
ACCAGG907830.3538140246106586No Hit
AACATA905910.35306573150814774No Hit
GTACGC905420.3528747608726111No Hit
CACATT894750.34871627784980314No Hit
CATACG890880.3472079995650546No Hit
AGCCTA889520.34667795861744277No Hit
AGAGGT885670.34517747505251206No Hit
AGGTGT864090.3367669723690823No Hit
CTATGC858890.33474034521644863No Hit
ACTAGC833270.3247553091298189No Hit
GGTAAT831760.3241668077835734No Hit
GGCCAG828620.32294303677217534No Hit
GGAGGC828170.32276765557627435No Hit
GGCGTT828160.32276375821636544No Hit
GACCGA809230.3153860559087971No Hit
GTTGGC802570.3127904142094624No Hit
GGTGGT795230.30992975203632184No Hit
AGTTCC794930.3098128312390545No Hit
TAATGA792380.3088190044622822No Hit
CCTCGG789270.3076069255306109No Hit
ATGCCG788800.3074237496148921No Hit
CTTAGC766200.2986157162207535No Hit
TGCATA764490.29794926767632973No Hit
TCTCGC756220.2947261510316604No Hit
AGGACT748470.2917056971022544No Hit
CCTTAT748030.29153421326626233No Hit
CGTTGC747950.2915030343869911No Hit
CCGACT735870.2867950236170267No Hit
CGTCAG733670.28593760443706634No Hit
CTCCGC730550.2847216281454861No Hit
ATGGCC726650.28320165778101086No Hit
AAGCAT717610.27967844442335543No Hit
TGCGGA717220.27952644738690785No Hit
CAGCCT714370.27841569981286823No Hit
CAGTAG706150.2752120699677435No Hit
AATGTA694250.27057421167613954No Hit
GTCGAG689760.26882429707703853No Hit
ACGCTA689390.2686800947604088No Hit
CTCGTC674250.2627794918583177No Hit
AAGCGC671720.26179345980136326No Hit
TCTCTG666180.2596343224118266No Hit
TCAATG643070.25062752366233354No Hit
CATGAG640340.24956354440720083No Hit
AAGGCG635940.24784870604728004No Hit
AGACGG615100.23972660799710974No Hit
GTCTCA611300.23824561123172355No Hit
TTACGT608400.23711537685813944No Hit
GAATAC607460.23674902502670178No Hit
GTTACA597170.23273864168043248No Hit
GAGCAC594330.23163179146630178No Hit
GCATAA593240.23120697923623046No Hit
AACTAG589470.2297376745505711No Hit
CTTCAC589460.22973377719066215No Hit
TTGAAT586620.22862692697653147No Hit
GTACTT582680.22709136717242057No Hit
NNNNNN580440.22621835855282454No Hit
TACAGT578930.22562985720657897No Hit
CGTGAA565020.2202086295732839No Hit
CGTCTC559850.21819369450037698No Hit
TTCGTT554630.2161592726279255No Hit
GCTTCT554280.2160228650311136No Hit
TGTGTG538970.21005600701057098No Hit
TCCAGA521140.2031070142929829No Hit
ACGTAA519050.20229246607202048No Hit
GAGTCG495480.19310638876671748No Hit
GCGGCT488070.1902184450742145No Hit
TAGAGC487280.18991055364141052No Hit
GGCAGG477960.18627821420630558No Hit
AGTAGG470650.1834292441128917No Hit
TACGGC469940.18315253155935904No Hit
CCTGAC453850.1768816794659214No Hit
CAACGG432190.16843999790322037No Hit
AAGTTG430330.16771508896016293No Hit
ACAGCC383500.14946375250673316No Hit
TGACAC365970.14263168058641237No Hit
CAAGGA313720.12226797506235287No Hit
TATGGT313570.12220951466371922No Hit
AACTCT304630.11872527490515286No Hit
GAGTGA277690.10822578731054691No Hit
TTAGCG273390.10654992254971521No Hit
GGTACC267740.10434791420118054No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)