FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25116622
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAG6095402.4268390868803933No Hit
TCTCTG5342432.127049569006533No Hit
TTACGT5330202.1221802836384605No Hit
ACCGCT4976551.9813771135306333No Hit
GTACGC4618961.8390052611374255No Hit
CGTCAG4608821.8349680940374864No Hit
GGAGGC4402361.7527675497126964No Hit
TTGAAT3989901.5885496067106475No Hit
CCATGA3468691.381033643775823No Hit
ACGCTA3412331.3585943205260642No Hit
AATCGG3277561.304936627226384No Hit
AATTGC3252731.2950507436867902No Hit
CTCGTC3151271.2546551841246805No Hit
CGTGAA3148941.253727511605661No Hit
CAAGCT3058101.2175602276452622No Hit
CTATTA2992341.1913783629024635No Hit
TTAGTA2947121.1733743494646693No Hit
CACCTA2929571.1663869448686213No Hit
GCACAT2856881.1374459511314856No Hit
AGGCTT2841351.1312627948137293No Hit
CGAATT2672741.0641319521391053No Hit
CGAGAG2609841.0390887755526996No Hit
AGATGC2605271.037269263358743No Hit
AAGGCG2492010.992175619794732No Hit
ATTCGA2487010.9901849062346044No Hit
ATCCAC2211760.8805961247495782No Hit
CATAAC2186270.8704474670200475No Hit
ACCTGC2139500.8518263323786137No Hit
CCTGCT2062370.8211175850000849No Hit
GGACTA2062040.8209861979051164No Hit
GTAAGA1965030.7823623734115202No Hit
GGATTC1927230.7673125788969551No Hit
AGTCAA1773890.7062613754349609No Hit
GACCGA1773160.7059707312551823No Hit
TATTCT1765650.7029806794878706No Hit
CACATT1736060.6911996366390353No Hit
CTGAGG1703600.6782759242066867No Hit
ATTGCT1694930.6748240268934255No Hit
CGCTTG1690020.67286914617738No Hit
CTATGC1665370.6630549283259508No Hit
TGGCAT1661680.6615857817185766No Hit
GAGCCA1649600.6567762177573082No Hit
GCCTGG1639430.6527271063760087No Hit
GAGATA1615220.6430880713178707No Hit
TCATGT1568670.6245545280730824No Hit
GGTACC1565040.6231092700284299No Hit
CGAGCC1561550.6217197519634607No Hit
GATCCT1534680.6110216572913347No Hit
TCGTGG1502320.5981377591301889No Hit
GGATCG1473500.5866632861696132No Hit
TGATAA1453590.5787362647731848No Hit
CTGGAG1430770.5696506480847624No Hit
ACGAAC1422720.5664455992529569No Hit
CATTAA1401820.5581244165716234No Hit
GCATAA1393670.5548795534686154No Hit
GTGGTG1366920.5442292359219325No Hit
AACCTT1356380.5400328117371834No Hit
GATTCA1352040.5383048723669926No Hit
AAGAGG1340640.5337660454499017No Hit
TTCTAC1332850.5306645137232228No Hit
AGTAGG1324890.5274952977354996No Hit
TATTGG1315520.5237647005238205No Hit
GGCCGT1281610.5102636811590349No Hit
ACACGA1273970.5072218708391598No Hit
AGGACT1259270.5013691729723846No Hit
TCCACG1245730.495978320651559No Hit
CCACTT1227630.4887719375638969No Hit
AAGCAT1225750.48802342926528897No Hit
GAAGAT1207740.48085287902170915No Hit
GGTAAT1202050.47858744699028394No Hit
TGTGTG1200930.4781415271528154No Hit
CATACG1158490.46124435045445206No Hit
TCAATG1157770.46095768770179363No Hit
TGTGAT1140870.4542290758685622No Hit
TCAAGC1125690.44818526950001475No Hit
TCCAGA1107030.44075592649361844No Hit
ACAGCC1104620.4397964025576369No Hit
ATCAGC1102850.4390916899573517No Hit
GTTACA1082160.4308541172455436No Hit
CTCCGC1047150.4169151408975299No Hit
CAGGAC1042460.41504785157813023No Hit
TAAGTC1039490.41386536772341437No Hit
TACTCG1034640.41193437557009055No Hit
ATCCGG1016990.40490715670284005No Hit
GCTTCT1014810.40403920559062445No Hit
AGAGGT997250.3970478195674562No Hit
ACTAGC989530.3939741578306191No Hit
TCTCGC973790.38770739154333733No Hit
TAATGA972880.3873450816753941No Hit
CGTCTC969490.3859953778816276No Hit
CAACGG968430.38557334660688053No Hit
AGGTGT936580.3728925012288675No Hit
TAGCTT926890.3690344983493401No Hit
TTCACA922070.3671154504773771No Hit
TTCTGT917230.36518843975117354No Hit
GTCGAG917090.36513269977149No Hit
TGCGAC913070.36353216606914734No Hit
CGTACT905570.3605460957289559No Hit
TATGGT899870.3582766822704104No Hit
ACTTGA893520.3557484760490483No Hit
CTTGTA888840.35388516815676885No Hit
AATGTA882620.35140872048797006No Hit
AAGCGC878240.34966485540929826No Hit
CTAGTT869050.34600592388578366No Hit
TTAGGC867130.3452414898786947No Hit
CTTAGC830690.3307331694524845No Hit
CTGTTC817090.32531842856893733No Hit
GAGCAC814550.3243071460803925No Hit
TACGGC796090.31695743161640133No Hit
ACGTAA790500.3147318138561786No Hit
AACTAG785620.312788877421494No Hit
TGCATA778360.3098983613321887No Hit
GATCAG777430.3095280886100049No Hit
CAGCCT768130.30582536138816757No Hit
CCAGCG748150.29787047000189754No Hit
CGTTGC742820.2957483693468015No Hit
ATTGAA736480.29322414455255963No Hit
GAATAC734210.2923203605962617No Hit
GCCAAT731660.2913050966805966No Hit
AAGTTG729800.29056455123622915No Hit
ACAGTG726230.28914318175429804No Hit
CCTCGG721400.28722015245521476No Hit
GAGTCG719370.2864119227498029No Hit
CTGTAT712320.2836050166300229No Hit
GCTCAA707870.28183328156150933No Hit
TATAAG704920.280658760561034No Hit
AGTTCC700980.27909007827565346No Hit
CCGCTC699100.27834156997704546No Hit
TACAGT697530.2777164859191654No Hit
TCTTCA673090.26798587803726154No Hit
ATACCT672180.2676235681693183No Hit
GACCAT671730.2674444039489068No Hit
AGAACG665260.2648684206021017No Hit
GTACTT657530.2617907774381444No Hit
GGCAGG655810.26110597197346047No Hit
CAGTCC654870.26073171782415644No Hit
GGCTAC653940.2603614451019727No Hit
GCGCAG638000.2540150502722858No Hit
ACCAGG636170.2532864491092791No Hit
GTCTCA630790.25114444131858177No Hit
AGCCTA624660.2487038264938653No Hit
CAGATC621130.24729838272041518No Hit
CTACCG620470.24703560853047835No Hit
ATGTGA618630.24630302594035136No Hit
ATTCAT611630.24351602695617267No Hit
GGCGTT607070.2417004961893363No Hit
GCGTAT600990.23927978850022108No Hit
CTTCAC594400.2366560280279729No Hit
AACTCT594380.23664806517373235No Hit
GAGTGA579080.2305564816797418No Hit
CCTTAT578010.2301304689778745No Hit
CCGGAA576670.22959695774376032No Hit
AGGTCA576070.22935807211654496No Hit
CAAGGA548110.21822600188831126No Hit
TAGAGC542550.21601232840944934No Hit
TGACAC538970.21458697750039793No Hit
TTCGTT529800.21093600883112387No Hit
TGACCA527940.2101954633867564No Hit
CCTGAC527250.20992074491545878No Hit
GCTGTC525310.20914834805412924No Hit
TGCGGA523670.2084953940064074No Hit
GGTGGT521410.2075955914772297No Hit
AGTTAG506520.20166724649516962No Hit
GACTAA494940.197056753889914No Hit
AGTGCA451680.17983310016768975No Hit
GCCATA450310.17928764465221478No Hit
AACAAT450090.17920005325556915No Hit
CAGTAG449940.17914033184876532No Hit
GTCTGC441840.17591537588135855No Hit
CGACAA430790.17151589891347652No Hit
TCGGAC429120.17085100058439387No Hit
CGAAGC425210.16929426258037406No Hit
CCGACT423770.1687209370750573No Hit
ACTAAT414440.16500626557185916No Hit
TTAGCG404130.16090141421087598No Hit
TACGAA395710.15754905257562105No Hit
GAACCG395700.15754507114850078No Hit
GACTTG392810.156394438710747No Hit
AACATA385620.15353179261128347No Hit
AGACGG373750.1488058386195405No Hit
TTCCAT367760.14642096377450756No Hit
TCCTCC365350.14546143983852608No Hit
GACACG361290.1438449804277024No Hit
GGCCAG351730.1400387361007384No Hit
ATGCCG340340.13550389061076765No Hit
CCGTCG335860.13372021126089328No Hit
GTTGGC313990.12501283014889503No Hit
GCGGCT293880.11700618021006168No Hit
CATGAG293370.11680312742692867No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)