FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001729991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001729991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24526486
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGA7104712.8967500684769925No Hit
GAGATA6444502.627567601816257No Hit
GAGAAG5965522.4322766824403628No Hit
AACCTT5838102.3803246824677613No Hit
GCACAT5396022.200078723058819No Hit
CGTGAA5375802.19183457426392No Hit
CACATT5258782.1441228882115437No Hit
CCTGCT5023122.048039005669218No Hit
GGACTA4808601.9605743766147339No Hit
TTGAAT4772461.9458392857419526No Hit
CCATGA4723161.9257385668701177No Hit
TCGTGG4665991.9024290719836505No Hit
CAGCCT4622771.8848073058651778No Hit
CTGGAG4525531.8451603707110753No Hit
GTACGC4456441.8169908237160433No Hit
TTACGT4350701.7738782473771415No Hit
AACTAG4317961.76052941297828No Hit
ACGCTA3926531.600934597805817No Hit
AAGGCG3863681.5753092391629198No Hit
TACGGC3786891.5440002289769517No Hit
CGAATT3695991.5069382544242171No Hit
TATTCT3664561.494123536490307No Hit
ATCCAC3440121.4026143003119158No Hit
CGTCAG3413291.3916751058427204No Hit
AGATGC3240441.3212002730435985No Hit
CATAAC3193001.301857918007496No Hit
AGTCAA3176311.2950530296105198No Hit
GGAGGC3156791.2870942865602517No Hit
GAAGAT3014661.2291446887254864No Hit
CTCGTC2992761.2202155661434744No Hit
AGGCTT2877651.1732826300514472No Hit
ACCGCT2820931.1501566102865286No Hit
ACCTGC2802271.1425485085796636No Hit
ACGTAA2801451.1422141761359537No Hit
TCTCTG2730811.1134126592778109No Hit
AATCGG2571061.048278991128203No Hit
CCTCGG2545551.0378779903488824No Hit
GCATAA2490051.0152493920246055No Hit
GGTACC2418730.9861706238716789No Hit
CGAGCC2391190.9749419464329298No Hit
GGATTC2359360.9619641395020877No Hit
CACCTA2336080.9524723598806613No Hit
TAAGTC2312650.9429194218853856No Hit
TCCACG2253560.9188270998136464No Hit
TCAAGC2236130.9117204967723465No Hit
GTTACA2222250.9060613085788156No Hit
ACACGA2163700.8821891566529343No Hit
CAAGCT2138950.8720980249677839No Hit
ACGAAC2085990.8505050417740233No Hit
AAGAGG2071710.8446827645835607No Hit
ACTAGC1978480.8066707966236989No Hit
TTAGTA1966190.8016598871929718No Hit
AGGACT1952670.7961474790966794No Hit
GACCGA1851660.7549634301464955No Hit
TGCATA1775700.7239928296291609No Hit
CATTAA1765230.719723975134473No Hit
GATCCT1765110.7196750484353934No Hit
GGATCG1733140.7066401603556254No Hit
AAGCAT1726550.7039532691311752No Hit
CAGGAC1696180.691570737039134No Hit
GAGCCA1686720.6877136822616986No Hit
GAGTCG1662070.6776633228257811No Hit
CAACGG1588530.6476794107398833No Hit
CATACG1561690.6367361390457646No Hit
TATTGG1535040.6258703346251885No Hit
TCAATG1532920.6250059629414503No Hit
TAATGA1509410.6154204071467881No Hit
ACAGCC1376530.561242242366069No Hit
CTGTTC1371740.5592892516278117No Hit
CCTTAT1353450.5518320072431085No Hit
TGTGTG1328640.5417164122084183No Hit
AGTAGG1328330.5415900182357961No Hit
TATGGT1325250.540334232959422No Hit
CTATGC1318940.537761504032824No Hit
GAGCAC1277580.5208981017500836No Hit
TGGCAT1256740.5124011650099407No Hit
GCTTCT1209210.49302211494952847No Hit
GAGTGA1196920.48801120551880117No Hit
ATGCCG1191520.4858095040602229No Hit
AGCCTA1103630.4499747742093996No Hit
TTCGTT1042100.424887609256377No Hit
GAATAC1037890.42317109756367055No Hit
CCGACT1024110.4175526816193726No Hit
AATGTA932950.38038469921863244No Hit
ATGGCC872100.3555747855603938No Hit
AGACGG717590.29257758327059163No Hit
GGTGGT700780.2857237681745359No Hit
AACAAT692380.28229889923896967No Hit
CAGTAG583620.23795500097323358No Hit
AAGTTG580940.23686230469379105No Hit
CGTTGC561940.22911557733953408No Hit
GGCGTT524190.21372405325410251No Hit
GGCCAG443860.18097170544528882No Hit
ATCCGG372860.1520234084899076No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)