FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001745296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087016
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC137830.44648294663843663No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA120780.3912516164477282No Hit
CCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGG112580.3646887479689124No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC89280.28921132899861873No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC84040.27223700816581453No Hit
CACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGA79940.2589555739264066No Hit
CTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACA69170.22406751374142536No Hit
CCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCAC68850.2230309139959106No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC68400.22157319560378047No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC65510.21221140415210027No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT64090.20761149278137855No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA63310.20508478090168628No Hit
GATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACC60770.19685677042166286No Hit
CTTCTTCAATCAGCACCACTGAGACCACATCCCACAGTACTCCCAGCTTC56960.18451475470162773No Hit
CTTCTTCGATCACCACCACCGAGACCACATCCCACAGTACTCCCAGCTTC56140.18185846785374615No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG56110.18176128662760413No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC52890.17133050168836184No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC52680.17065023310536775No Hit
CCACATCCCACAGTACTCCCAGCTTCACTTCTTTGATCACCACCACGGAG51810.16783197754724952No Hit
CCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCA49160.1592476359047054No Hit
CATCCCACAGTACTCCCAGCTTCACTTCTTTGATCACCACCACGGAGACC49060.15892369848423202No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA48970.158632154805806No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC48600.15743358635005456No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT46280.14991823819507252No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA46140.14946472580640982No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT45740.14816897612451638No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT43490.1408803841638657No Hit
CCCAGCTTCACTTCTTTGATCACCACCACGGAGACCACCTCACACAGTAC42230.13679877266590132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42140.13650722898747528No Hit
AGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC41490.13440163575439842No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC40830.1322636487792742No Hit
GATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTC39540.12808485605516784No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC38820.12575250662775964No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC38730.1254609629493336No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG38260.12393845707310878No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC37640.12193004506617394No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA37290.12079626409451716No Hit
CTCACACAATACTCCCAGCTTCACTTCTTCGATCACCACCACCGAGACCA36420.11797800853639889No Hit
CCTCACACAATACTCCCAGCTTCACTTCTTCGATCACCACCACCGAGACC36100.11694140879088413No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC36060.11681183382269479No Hit
CCACCTCACACAATACTCCCAGCTTCACTTCTTCGATCACCACCACCGAG35830.11606677775560606No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT35660.11551608414080135No Hit
CTTCTTCAATCAGGACCACTGAGACCACCTCTCATAGTACTCCCAGCTTC35170.11392879078048186No Hit
GATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAG35120.11376682207024519No Hit
CCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCC35090.11366964084410316No Hit
TAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAG35070.11360485336000851No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC34060.11033308541322753No Hit
CCCCCTCACACAGTACTCCCAGCTCCACTTCTTTAATCACCACCACCAAG33650.10900494198928674No Hit
GATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACC33630.10894015450519208No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC33580.10877818579495539No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG33160.10741764862896726No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT33110.10725567991873058No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT33060.10709371120849391No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA32880.10651062385164185No Hit
CCACCACCGACTCGATCGTCACCACCGAGACCACATCCCACAGTACTCCC32810.10628386765731049No Hit
CCCACAGTACTCCCAGCTTCACTTCTTTGATCACCACCACGGAGACCACC32720.10599232397888446No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT32240.10443742436061232No Hit
CACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACT31900.10333603713100288No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT31710.1027205560321035No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT31150.10090650647745267No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)