FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001745310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986266
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA65160.32805273815289593No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT63130.3178325561631725No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT42840.21568108199002553No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC41550.20918648358276282No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT35010.1762603800296637No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC30900.1555682874297803No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA30690.15451102722394686No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG29090.14645571136997765No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC27840.1401624958590642No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC27640.13915558137731804No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC27020.13603414648390497No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC26750.13467481193354766No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC26430.13306374876275384No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC26360.13271132869414268No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT26270.1322582171773569No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC25360.12767675628541192No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC25030.12601534739053077No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA24950.1256125815978323No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC24770.12470635856426078No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC24230.12198768946354618No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA23300.11730553712342656No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT23080.11619793119350581No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT23010.11584551112489465No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC22100.11126405023294966No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC21980.11065990154390198No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA21380.10763915809866352No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA21240.10693431796144123No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC21190.10668258934100469No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC21100.1062294778242189No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA20820.1048197975497743No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC20150.1014466340359247No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC19950.10043971955417855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)