FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001745313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8424074
Sequences flagged as poor quality0
Sequence length126
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG242700.28810288228712144No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG183710.218077381561463No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC173010.20537568877006543No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA168070.19951154275235475No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT165970.1970186871577814No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG158700.18838865850418693No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT156050.18524291215865388No Hit
GTGTGACCTGAGGATGCTGAGGAAGGGCTGGTGACAGGAAGAGGGGTGGT155100.1841151917706326No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT147140.1746660819931069No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT143290.17009584673638906No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT139770.16591734593024704No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG136990.16261727995266897No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT125170.14858606417749892No Hit
CTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGTC118280.14040712367911298No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG116320.13808045845751118No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA113350.13455484840232884No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGTAGATGCTGAGGAAGGGC112930.13405627728341418No Hit
CTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCT109970.1305425379691584No Hit
CAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACA107510.1276223357012296No Hit
GGGTGGTGTCACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGG106390.12629281271745715No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA101360.12032183003140762No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGAGGATGCTGAGGAAGGGC101230.12016751039936259No Hit
GGTGGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGG97050.11520554069206894No Hit
GTCGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGCGT93810.11135942063187004No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC93610.11112200581333924No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA91610.10874785762803127No Hit
CTGGTGACAGGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGCT91160.10821367428633698No Hit
GTCGGTGACAGGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT88270.10478303015856699No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC88260.10477115941764045No Hit
GTCGGTGACAAGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGC85360.10132864454894389No Hit
GTCGGTGACAAGAAGAGAGGTGGCGTGACCTGTGGATACTGAGGAAGCGT84270.10003473378795105No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)