FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001745328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5242516
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1068292.037742946325772No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA153130.29209257539700406No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT137250.2618017760937687No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT102660.19582200607494568No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC88880.16953691700702486No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT84260.16072435448933298No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC69070.13174971712055814No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC64320.12268918206448963No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT62020.11830197561628805No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG61430.11717656178827113No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC60460.11532630515576872No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT59470.11343789890197759No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG56470.10771545570867117No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT53440.10193578808343169No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)